Key features and details
- Rabbit monoclonal [C2D4] to Cyclophilin B
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-Cyclophilin B antibody [C2D4]
See all Cyclophilin B primary antibodies
DescriptionRabbit monoclonal [C2D4] to Cyclophilin B
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Human
- WB: HeLa, HepG2 and NIH/3T3 cell extracts.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
Our Abpromise guarantee covers the use of ab238511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 24 kDa.|
FunctionPPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Involvement in diseaseDefects in PPIB are the cause of osteogenesis imperfecta type 9 (OI9) [MIM:259440]. OI9 is a connective tissue disorder characterized by bone fragility, low bone mass and bowing of limbs due to multiple fractures. Short limb dwarfism and blue sclerae are observed in some but not all patients.
Sequence similaritiesBelongs to the cyclophilin-type PPIase family. PPIase B subfamily.
Contains 1 PPIase cyclophilin-type domain.
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- AA408962 antibody
- AA553318 antibody
- AI844835 antibody
All lanes : Anti-Cyclophilin B antibody [C2D4] (ab238511) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) cell extract
Lysates/proteins at 25 µg per lane.
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 24 kDa
Blocking buffer: 3% non-fat dry milk in TBST.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab238511 has not yet been referenced specifically in any publications.