Anti-Cyclophilin B antibody [C2D4] (ab238511)
Key features and details
- Rabbit monoclonal [C2D4] to Cyclophilin B
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Cyclophilin B antibody [C2D4]
See all Cyclophilin B primary antibodies -
Description
Rabbit monoclonal [C2D4] to Cyclophilin B -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to Human Cyclophilin B aa 150 to the C-terminus.
Database link: P23284 -
Positive control
- WB: HeLa, HepG2 and NIH/3T3 cell extracts.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
C2D4 -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab238511 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 24 kDa.
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Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 24 kDa. |
Target
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Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
Involvement in disease
Defects in PPIB are the cause of osteogenesis imperfecta type 9 (OI9) [MIM:259440]. OI9 is a connective tissue disorder characterized by bone fragility, low bone mass and bowing of limbs due to multiple fractures. Short limb dwarfism and blue sclerae are observed in some but not all patients. -
Sequence similarities
Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.
Contains 1 PPIase cyclophilin-type domain. -
Cellular localization
Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 5479 Human
- Entrez Gene: 19035 Mouse
- Omim: 123841 Human
- SwissProt: P23284 Human
- SwissProt: P24369 Mouse
- Unigene: 434937 Human
- Unigene: 335249 Mouse
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Alternative names
- AA408962 antibody
- AA553318 antibody
- AI844835 antibody
see all
Images
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All lanes : Anti-Cyclophilin B antibody [C2D4] (ab238511) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : HepG2 (human liver hepatocellular carcinoma cell line) cell extract
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) cell extract
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : HRP Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Predicted band size: 24 kDaBlocking buffer: 3% non-fat dry milk in TBST.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab238511 has not yet been referenced specifically in any publications.