Product nameAnti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free
See all Cyclophilin B primary antibodies
DescriptionRabbit monoclonal [EPR12703(B)] to Cyclophilin B - BSA and Azide free
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human Cyclophilin B aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
Database link: P23284
- WB: HepG2, HeLa and A431 cell lysate.
Ab238991 is the carrier-free version of ab178397. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab238991 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: PBS
Concentration information loading...
PurityProtein A purified
- HeLa whole cell lysate (ab150035)
- HepG2 whole cell lysate (ab166833)
- HeLa whole cell lysate (ab29545)
- A431 whole cell lysate (ab30132)
- Mouse heart normal tissue lysate - total protein (ab30291)
- NIH 3T3 whole cell lysate (ab7179)
- Mouse heart tissue lysate - total protein (0 days) (ab7193)
- Mouse heart tissue lysate - total protein (14 days) (ab7194)
- Mouse heart tissue lysate - total protein (7 days) (ab7196)
- HepG2 whole cell lysate (ab7900)
- A431 whole cell lysate (ab7909)
Our Abpromise guarantee covers the use of ab238991 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.|
FunctionPPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Involvement in diseaseDefects in PPIB are the cause of osteogenesis imperfecta type 9 (OI9) [MIM:259440]. OI9 is a connective tissue disorder characterized by bone fragility, low bone mass and bowing of limbs due to multiple fractures. Short limb dwarfism and blue sclerae are observed in some but not all patients.
Sequence similaritiesBelongs to the cyclophilin-type PPIase family. PPIase B subfamily.
Contains 1 PPIase cyclophilin-type domain.
Cellular localizationEndoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
- Information by UniProt
- AA408962 antibody
- AA553318 antibody
- AI844835 antibody
All lanes : Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control (ab178397) at 1/1000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : A431 (Human epidermoid carcinoma cell line) cell lysate
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 24 kDa
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab178397).
ab238991 has not yet been referenced specifically in any publications.