Recombinant
RabMAb

Recombinant Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free (ab238991)

Overview

  • Product name

    Anti-Cyclophilin B antibody [EPR12703(B)] - BSA and Azide free
    See all Cyclophilin B primary antibodies
  • Description

    Rabbit monoclonal [EPR12703(B)] to Cyclophilin B - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human Cyclophilin B aa 150 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P23284

  • Positive control

    • WB: HepG2, HeLa and A431 cell lysate.
  • General notes

    Ab238991 is the carrier-free version of ab178397. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab238991 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab238991 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 24 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
    • Involvement in disease

      Defects in PPIB are the cause of osteogenesis imperfecta type 9 (OI9) [MIM:259440]. OI9 is a connective tissue disorder characterized by bone fragility, low bone mass and bowing of limbs due to multiple fractures. Short limb dwarfism and blue sclerae are observed in some but not all patients.
    • Sequence similarities

      Belongs to the cyclophilin-type PPIase family. PPIase B subfamily.
      Contains 1 PPIase cyclophilin-type domain.
    • Cellular localization

      Endoplasmic reticulum lumen. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • Database links

    • Alternative names

      • AA408962 antibody
      • AA553318 antibody
      • AI844835 antibody
      • Cphn 2 antibody
      • Cphn2 antibody
      • Cyclophilin B antibody
      • Cyclophilin like protein antibody
      • CyP 20b antibody
      • CYP S1 antibody
      • CYP-S1 antibody
      • CYPB antibody
      • EC 5.2.1.8 antibody
      • MGC14109 antibody
      • MGC2224 antibody
      • OI9 antibody
      • peptidyl prolyl cis trans isomerase B antibody
      • Peptidyl prolyl cis trans isomerase B precursor antibody
      • Peptidyl-prolyl cis-trans isomerase B antibody
      • peptidylprolyl isomerase B (cyclophilin B) antibody
      • Peptidylprolyl isomerase B antibody
      • PPIase antibody
      • PPIase B antibody
      • Ppib antibody
      • PPIB_HUMAN antibody
      • Rotamase antibody
      • Rotamase B antibody
      • S cyclophilin antibody
      • S-cyclophilin antibody
      • SCYLP antibody
      see all

    Images

    • All lanes : Anti-Cyclophilin B antibody [EPR12703(B)] - Loading Control (ab178397) at 1/1000 dilution

      Lane 1 : HepG2 (Human liver hepatocellular carcinoma cell line) cell lysate
      Lane 2 : HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
      Lane 3 : A431 (Human epidermoid carcinoma cell line) cell lysate

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 24 kDa



      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab178397).

    References

    ab238991 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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