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Cell Biology Apoptosis Mitochondrial
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Validated using a knockout cell line

Anti-Cyclophilin F antibody (ab126573)

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  • SDS
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Western blot - Anti-Cyclophilin F antibody (ab126573)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin F antibody (ab126573)
  • Western blot - Anti-Cyclophilin F antibody (ab126573)

Key features and details

  • Rabbit polyclonal to Cyclophilin F
  • Suitable for: ICC/IF, WB
  • Knockout validated
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Protein
Product image
Recombinant human Cyclophilin F protein (ab79186)

View more associated products

Overview

  • Product name

    Anti-Cyclophilin F antibody
    See all Cyclophilin F primary antibodies
  • Description

    Rabbit polyclonal to Cyclophilin F
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Chimpanzee, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Cyclophilin F aa 1-100 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab156630)

  • Positive control

    • WB: Human heart and heart mitochondrial tissue lysates and HeLa, HepG2, HEK293 and HL60 whole cell lysates. ICC/IF: A431 cells.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    • Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Cyclophilin F protein (ab79186)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126573 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Notes
ICC/IF
Use a concentration of 5 µg/ml.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

Target

  • Function

    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • Sequence similarities

    Belongs to the cyclophilin-type PPIase family.
    Contains 1 PPIase cyclophilin-type domain.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession P30405 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10105 Human
    • Omim: 604486 Human
    • SwissProt: P30405 Human
    • Unigene: 381072 Human
    • Form

      This gene encodes a 178 aa mature protein that is found in the mitochondrion and may participate in the permeability transition pore. While technically this protein is Cyclophilin F, literature references commonly refer to this protein as 'cyclophilin D’ or ‘CypD’. A different cytoplasmic protein of 370 aa, represented by Entrez GeneID 5481, is identified as Cyclophilin D. This antibody does not react with this 370 aa cytoplasmic protein.
    • Alternative names

      • Cyclophilin 3 antibody
      • cyclophilin D antibody
      • Cyclophilin F antibody
      • Cyp D antibody
      • CyP M antibody
      • CyP-D antibody
      • CyP-M antibody
      • CYP3 antibody
      • CypD antibody
      • hCyP3 antibody
      • mitochondrial antibody
      • Mitochondrial cyclophilin antibody
      • Peptidyl prolyl cis trans isomerase, mitochondral antibody
      • Peptidyl-prolyl cis-trans isomerase F antibody
      • Peptidyl-prolyl cis-trans isomerase F, mitochondrial antibody
      • Peptidylprolyl isomerase F (cyclophilin F) antibody
      • Peptidylprolyl isomerase F antibody
      • PPIase antibody
      • PPIase F antibody
      • Ppif antibody
      • PPIF_HUMAN antibody
      • Rotamase antibody
      • Rotamase F antibody
      see all

    Images

    • Western blot - Anti-Cyclophilin F antibody (ab126573)
      Western blot - Anti-Cyclophilin F antibody (ab126573)

      Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
      Lane 2: Cyclophilin F knockout HAP1 whole cell lysate (20 µg)
      Lane 3: HeLa whole cell lysate (20 µg)
      Lane 4: HEK293 whole cell lysate (20 µg)              

      Lanes 1 - 4: Merged signal (red and green). Green - ab126573 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab126573 was shown to specificaly recognize Cyclophilin F in wild-type HAP1 cells along with additional cross-reactive bands. No bands was observed when Cyclophilin F knockout samples were examined. Wild-type and Cyclophilin F knockout samples were subjected to SDS-PAGE.  Ab126573 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging. 

    • Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin F antibody (ab126573)
      Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin F antibody (ab126573)

      ICC/IF image of ab126573 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab126573, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Western blot - Anti-Cyclophilin F antibody (ab126573)
      Western blot - Anti-Cyclophilin F antibody (ab126573)
      All lanes : Anti-Cyclophilin F antibody (ab126573) at 1 µg/ml

      Lane 1 : Human Heart Mitochondrial Lysate
      Lane 2 : Human heart tissue lysate - total protein (ab29431)
      Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
      Lane 4 : HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate
      Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 6 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 22 kDa
      Observed band size: 22 kDa
      Additional bands at: 48 kDa, 61 kDa, 96 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 30 seconds


      This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab126573 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

    Protocols

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab126573? Please let us know so that we can cite the reference in this datasheet.

    ab126573 has not yet been referenced specifically in any publications.

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