Overview

  • Product name

    Anti-Cyclophilin F antibody
    See all Cyclophilin F primary antibodies
  • Description

    Rabbit polyclonal to Cyclophilin F
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 21-157 of Human Cyclophilin F (P30405).

  • Positive control

    • 293T and A431 whole cell lysates; Human colon carcinoma tissue; A431 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab127184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 22 kDa.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/500.

Target

  • Function

    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • Sequence similarities

    Belongs to the cyclophilin-type PPIase family.
    Contains 1 PPIase cyclophilin-type domain.
  • Cellular localization

    Mitochondrion matrix.
  • Information by UniProt
  • Database links

  • Form

    This gene encodes a 178 aa mature protein that is found in the mitochondrion and may participate in the permeability transition pore. While technically this protein is Cyclophilin F, literature references commonly refer to this protein as 'cyclophilin D’ or ‘CypD’. A different cytoplasmic protein of 370 aa, represented by Entrez GeneID 5481, is identified as Cyclophilin D. This antibody does not react with this 370 aa cytoplasmic protein.
  • Alternative names

    • Cyclophilin 3 antibody
    • cyclophilin D antibody
    • Cyclophilin F antibody
    • Cyp D antibody
    • CyP M antibody
    • CYP3 antibody
    • CypD antibody
    • hCyP3 antibody
    • mitochondrial antibody
    • Mitochondrial cyclophilin antibody
    • Peptidyl prolyl cis trans isomerase, mitochondral antibody
    • Peptidyl-prolyl cis-trans isomerase F antibody
    • Peptidyl-prolyl cis-trans isomerase F, mitochondrial antibody
    • Peptidylprolyl isomerase F (cyclophilin F) antibody
    • Peptidylprolyl isomerase F antibody
    • PPIase antibody
    • PPIase F antibody
    • Ppif antibody
    • PPIF_HUMAN antibody
    • Rotamase antibody
    • Rotamase F antibody
    see all

Images

  • Anti-Cyclophilin F antibody (ab127184) at 1/1000 dilution + A431 whole cell lysate at 30 µg

    Predicted band size: 22 kDa



    12% SDS PAGE
  • ab127184, at 1/250 dilution, staining Cyclophilin F in paraffin-embedded Human colon carcinoma tissue by Immunohistochemistry.
  • ab127184, at 1/500 dilution staining Cyclophilin F in methanol-fixed A431 cells by Immunofluorescence. The lower image is stained with Hoechst 33342.

References

ab127184 has not yet been referenced specifically in any publications.

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