Anti-Cyclophilin F antibody (ab64935)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-Cyclophilin F antibody
See all Cyclophilin F primary antibodies -
Description
Rabbit polyclonal to Cyclophilin F -
Host species
Rabbit -
Specificity
ab64935 detects endogenous levels of total Cyclophilin F protein. -
Tested applications
Suitable for: IHC-P, ICC/IF, WB, ELISAmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide derived from an internal sequence within Human Cyclophilin F.
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Positive control
- Human brain tissue and extracts from HeLa cells, 293 cells and HepG2 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab64935 was affinity-purified from rabbit antiserum by affinity-chromatography using an epitope-specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab64935 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/100. | |
| ICC/IF | Use a concentration of 1 - 5 µg/ml. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). | |
| ELISA | 1/20000. |
Target
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Function
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides. -
Sequence similarities
Belongs to the cyclophilin-type PPIase family.
Contains 1 PPIase cyclophilin-type domain. -
Cellular localization
Mitochondrion matrix. - Information by UniProt
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Database links
- Entrez Gene: 10105 Human
- Entrez Gene: 105675 Mouse
- Entrez Gene: 282819 Rat
- Omim: 604486 Human
- SwissProt: P30405 Human
- SwissProt: Q99KR7 Mouse
- SwissProt: P29117 Rat
- Unigene: 381072 Human
see all -
Form
This gene encodes a 178 aa mature protein that is found in the mitochondrion and may participate in the permeability transition pore. While technically this protein is Cyclophilin F, literature references commonly refer to this protein as 'cyclophilin D’ or ‘CypD’. A different cytoplasmic protein of 370 aa, represented by Entrez GeneID 5481, is identified as Cyclophilin D. This antibody does not react with this 370 aa cytoplasmic protein. -
Alternative names
- Cyclophilin 3 antibody
- cyclophilin D antibody
- Cyclophilin F antibody
see all
Images
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Western blot - Anti-Cyclophilin F antibody (ab64935)All lanes : Anti-Cyclophilin F antibody (ab64935) at 1/500 dilution
Lane 1 : Extracts from HeLa cells
Lane 2 : Extracts from 293 cells
Lane 3 : Extracts from HepG2 cells
Lane 4 : Extracts from HepG2 cells with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 22 kDa
Observed band size: 22 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin F antibody (ab64935)ab64935, at a 1/50 dilution, staining Human Cyclophilin F in paraffin-embedded brain tissue, by Immunohistochemistry, with (+) or without (-) immunising peptide. -
Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin F antibody (ab64935)ICC/IF image of ab64935 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64935, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Tadic V et al. Investigation of mitochondrial calcium uniporter role in embryonic and adult motor neurons from G93AhSOD1 mice. Neurobiol Aging 75:209-222 (2019). Read more (PubMed: 30597405) »
