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    cyclophilin-f-antibody-ab64935.pdf

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Cell Biology Apoptosis Mitochondrial
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Anti-Cyclophilin F antibody (ab64935)

  • Datasheet
  • SDS
Reviews (3)Q&A (1)References (3)

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Western blot - Anti-Cyclophilin F antibody (ab64935)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin F antibody (ab64935)
  • Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin F antibody (ab64935)

Key features and details

  • Rabbit polyclonal to Cyclophilin F
  • Suitable for: IHC-P, ICC/IF, WB
  • Reacts with: Human
  • Isotype: IgG

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Anti-Cyclophilin F antibody [EPR11311-121] (ab231155)

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Overview

  • Product name

    Anti-Cyclophilin F antibody
    See all Cyclophilin F primary antibodies
  • Description

    Rabbit polyclonal to Cyclophilin F
  • Host species

    Rabbit
  • Specificity

    ab64935 detects endogenous levels of total Cyclophilin F protein.
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide derived from an internal sequence within Human Cyclophilin F.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

    Without Mg2+ and Ca2+
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Purification notes

    ab64935 was affinity-purified from rabbit antiserum by affinity-chromatography using an epitope-specific immunogen.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Apoptosis
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Apoptosis
    • Metabolism
    • Types of disease
    • Cancer
    • Cancer
    • Cell Death
    • Apoptosis
    • Mitochondrial
    • Cancer
    • Cell Death
    • Apoptosis
    • Metabolism

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human Cyclophilin F protein (ab79186)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab64935 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
Application Abreviews Notes
IHC-P
1/50 - 1/100.
ICC/IF
Use a concentration of 1 - 5 µg/ml.
WB (2)
1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
Notes
IHC-P
1/50 - 1/100.
ICC/IF
Use a concentration of 1 - 5 µg/ml.
WB
1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

Target

  • Function

    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • Sequence similarities

    Belongs to the cyclophilin-type PPIase family.
    Contains 1 PPIase cyclophilin-type domain.
  • Cellular localization

    Mitochondrion matrix.
  • Target information above from: UniProt accession P30405 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10105 Human
    • Omim: 604486 Human
    • SwissProt: P30405 Human
    • Unigene: 381072 Human
    • Form

      This gene encodes a 178 aa mature protein that is found in the mitochondrion and may participate in the permeability transition pore. While technically this protein is Cyclophilin F, literature references commonly refer to this protein as 'cyclophilin D’ or ‘CypD’. A different cytoplasmic protein of 370 aa, represented by Entrez GeneID 5481, is identified as Cyclophilin D. This antibody does not react with this 370 aa cytoplasmic protein.
    • Alternative names

      • Cyclophilin 3 antibody
      • cyclophilin D antibody
      • Cyclophilin F antibody
      • Cyp D antibody
      • CyP M antibody
      • CyP-D antibody
      • CyP-M antibody
      • CYP3 antibody
      • CypD antibody
      • hCyP3 antibody
      • mitochondrial antibody
      • Mitochondrial cyclophilin antibody
      • Peptidyl prolyl cis trans isomerase, mitochondral antibody
      • Peptidyl-prolyl cis-trans isomerase F antibody
      • Peptidyl-prolyl cis-trans isomerase F, mitochondrial antibody
      • Peptidylprolyl isomerase F (cyclophilin F) antibody
      • Peptidylprolyl isomerase F antibody
      • PPIase antibody
      • PPIase F antibody
      • Ppif antibody
      • PPIF_HUMAN antibody
      • Rotamase antibody
      • Rotamase F antibody
      see all

    Images

    • Western blot - Anti-Cyclophilin F antibody (ab64935)
      Western blot - Anti-Cyclophilin F antibody (ab64935)
      All lanes : Anti-Cyclophilin F antibody (ab64935) at 1/500 dilution

      Lane 1 : Extracts from HeLa cells
      Lane 2 : Extracts from 293 cells
      Lane 3 : Extracts from HepG2 cells
      Lane 4 : Extracts from HepG2 cells with immunising peptide at 10 µg

      Lysates/proteins at 30 µg per lane.

      Predicted band size: 22 kDa
      Observed band size: 22 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin F antibody (ab64935)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cyclophilin F antibody (ab64935)
      ab64935, at a 1/50 dilution, staining Human Cyclophilin F in paraffin-embedded brain tissue, by Immunohistochemistry, with (+) or without (-) immunising peptide.
    • Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin F antibody (ab64935)
      Immunocytochemistry/ Immunofluorescence - Anti-Cyclophilin F antibody (ab64935)
      ICC/IF image of ab64935 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64935, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab64935? Please let us know so that we can cite the reference in this datasheet.

    ab64935 has been referenced in 3 publications.

    • Belosludtsev KN  et al. Transport of Ca2+ and Ca2+-Dependent Permeability Transition in the Liver and Heart Mitochondria of Rats with Different Tolerance to Acute Hypoxia. Biomolecules 10:N/A (2020). PubMed: 31936494
    • Tadic V  et al. Investigation of mitochondrial calcium uniporter role in embryonic and adult motor neurons from G93AhSOD1 mice. Neurobiol Aging 75:209-222 (2019). PubMed: 30597405
    • Belosludtsev KN  et al. Transport of Ca2+ and Ca2+-Dependent Permeability Transition in Rat Liver Mitochondria under the Streptozotocin-Induced Type I Diabetes. Cells 8:N/A (2019). PubMed: 31480399

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-4 of 4 Abreviews or Q&A

    Western blot abreview for Anti-Cyclophilin F antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Spermophilus tridecemlineatus Tissue lysate - other (Liver whole, cytosol, mitochondria)
    Gel Running Conditions
    Reduced Denaturing (12.5%)
    Loading amount
    20 µg
    Specification
    Liver whole, cytosol, mitochondria
    Blocking step
    BSA as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
    Read More

    Mr. James Bjork

    Verified customer

    Submitted Sep 25 2015

    Question

    Je souhaiterais savoir si vous aviez des informations de cross-réactivité avec les Cyclophilines A, B et C pour les anticorps anti-Cyclophiline F suivants :

    - ab86425

    - ab64935

    - ab3567

    - ab37664

    - ab110324

    Read More

    Abcam community

    Verified customer

    Asked on May 29 2013

    Answer


    Sachez que ces anticorps sont contre la cyclophiline F ', PPIF, CYP3, SwissProt: P30405. Toutefois, dans le domaine mitochondrial, cette protéine est généralement appelé CYPD ou cyclophiline D - une protéine de 25 kDa dans les mitochondries.
    Je peux vous confirmer qu’ab110324, ab64935 et ab3567 ne croisent pas avec les Cyclophilines A, B et C. Cependant, pour ab86425 et ab37664 nous n’avons pas déterminé si ces anticorps croisent avec les Cyclophilines A, B et C.

    Read More

    Abcam Scientific Support

    Answered on May 29 2013

    Western blot abreview for Anti-Cyclophilin F antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Heart)
    Loading amount
    20 µg
    Specification
    Heart
    Gel Running Conditions
    Reduced Denaturing (15)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 07 2011

    Immunoprecipitation abreview for Anti-Cyclophilin F antibody

    Poor
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - other (mitochondria from HCT116 cells)
    Total protein in input
    500 µg
    Specification
    mitochondria from HCT116 cells
    Immuno-precipitation step
    Protein A
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 11 2009

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