Anti-Cyclophilin F antibody [E11AE12BD4] (Alexa Fluor® 488) (ab156032)

Overview

  • Product name
    Anti-Cyclophilin F antibody [E11AE12BD4] (Alexa Fluor® 488)
    See all Cyclophilin F primary antibodies
  • Description
    Mouse monoclonal [E11AE12BD4] to Cyclophilin F (Alexa Fluor® 488)
  • Host species
    Mouse
  • Conjugation
    Alexa Fluor® 488. Ex: 495nm, Em: 519nm
  • Tested applications
    Suitable for: ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Recombinant Rat Cyclophilin F protein (UniProt: P29117).

  • Positive control
    • HDFn and HeLa cells.
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab156032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
Flow Cyt Use a concentration of 0.2 µg/ml.

ab171463 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
  • Sequence similarities
    Belongs to the cyclophilin-type PPIase family.
    Contains 1 PPIase cyclophilin-type domain.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Form
    This gene encodes a 178 aa mature protein that is found in the mitochondrion and may participate in the permeability transition pore. While technically this protein is Cyclophilin F, literature references commonly refer to this protein as 'cyclophilin D’ or ‘CypD’. A different cytoplasmic protein of 370 aa, represented by Entrez GeneID 5481, is identified as Cyclophilin D. This antibody does not react with this 370 aa cytoplasmic protein.
  • Alternative names
    • Cyclophilin 3 antibody
    • cyclophilin D antibody
    • Cyclophilin F antibody
    • Cyp D antibody
    • CyP M antibody
    • CYP3 antibody
    • CypD antibody
    • hCyP3 antibody
    • mitochondrial antibody
    • Mitochondrial cyclophilin antibody
    • Peptidyl prolyl cis trans isomerase, mitochondral antibody
    • Peptidyl-prolyl cis-trans isomerase F antibody
    • Peptidyl-prolyl cis-trans isomerase F, mitochondrial antibody
    • Peptidylprolyl isomerase F (cyclophilin F) antibody
    • Peptidylprolyl isomerase F antibody
    • PPIase antibody
    • PPIase F antibody
    • Ppif antibody
    • PPIF_HUMAN antibody
    • Rotamase antibody
    • Rotamase F antibody
    see all

Images

  • Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling Cyclophilin F with ab156032 at 5.0 µg/ml, (green); Anti-HSP60 (1/2500, ab46798) as red, and DAPI in blue, as a nuclear stain.
    Secondary antibody used was goat anti-rabbit Alexa594 (1/1000).
    All blocking and antibodies were diluted in 10% normal goat serum.
  • Immunofluorescent analysis of paraformaldehyde-fixed HDFn cells labeling Cyclophilin F with ab156032 at 5.0 µg/ml (green); Anti-HSP60 (1/2500, ab46798) as red, and DAPI in blue, as a nuclear stain.
    Secondary antibody used was goat anti-rabbit Alexa594 (1/1000).
    All blocking and antibodies were diluted in 10% normal goat serum
  • Flow cytometric analysis of HeLa cells labeling Cyclophilin F with ab156032 at 0.2 µg/ml (red) or an equal amount of IgG1 Alexa-488 Isotype control antibody (blue).

References

ab156032 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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