Product nameAnti-CYFIP1 antibody
See all CYFIP1 primary antibodies
DescriptionRabbit polyclonal to CYFIP1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Chimpanzee, Lizard, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 1203 and 1253 of Human CYFIP1 (NP_055423.1).
- HeLa and 293T whole cell lysates
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab108220 was affinity purified using an epitope specific to CYFIP1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab108220 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 145 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
FunctionComponent of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA (By similarity). Involved in formation of membrane ruffles and lamellipodia protrusions and in axon outgrowth. Binds to F-actin but not to RNA. Regulator of epithelial morphogenesis. May act as an invasion suppressor in cancers.
Sequence similaritiesBelongs to the CYFIP family.
Cellular localizationCytoplasm > perinuclear region. Cell projection > lamellipodium. Cell projection > ruffle. Cell junction > synapse > synaptosome. Highly expressed in the perinuclear region. Enriched in synaptosomes, membrane ruffles and at the tips of lamellipodia.
- Information by UniProt
- CY antibody
- CYFIP 1 antibody
- CYFIP1 antibody
All lanes : Anti-CYFIP1 antibody (ab108220) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
at 50 µg
Lane 2 : HeLa whole cell lysate
at 15 µg
Lane 3 : HeLa whole cell lysate
at 5 µg
Lane 4 : 293T whole cell lysate
at 50 µg
Developed using the ECL technique.
Predicted band size: 145 kDa
Exposure time: 3 minutes
ab108220 at 1 µg/ml detecting CYFIP1 in HeLa whole cell lysate by WB following IP.
Lane 1: IP with an antibody which recognizes an upstream epitope of CYFIP1
Lane 2: ab108220 at 6 µg/mg of lysate
Lane 3: Control IgG.
In each case, 1 mg of lysate was used for IP and 20% of the IP was loaded.
Detection: Chemiluminescence with an exposure time of 30 seconds
ab108220 has been referenced in 2 publications.
- Cioni JM et al. Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function. Neuron 97:1078-1093.e6 (2018). PubMed: 29518358
- Han K et al. Fragile X-like behaviors and abnormal cortical dendritic spines in cytoplasmic FMR1-interacting protein 2-mutant mice. Hum Mol Genet 24:1813-23 (2015). PubMed: 25432536