Recombinant
RabMAb

Recombinant Anti-CYFIP1 antibody [EPR10782] (ab156016)

Overview

  • Product name

    Anti-CYFIP1 antibody [EPR10782]
    See all CYFIP1 primary antibodies
  • Description

    Rabbit monoclonal [EPR10782] to CYFIP1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CYFIP1. The exact sequence is proprietary.
    Database link: Q7L576

  • Positive control

    • HeLa, 293T, A549 and Fetal brain lysates; HeLa cells; 293T cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR10782
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab156016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 145 kDa.
ICC/IF 1/250 - 1/500.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA (By similarity). Involved in formation of membrane ruffles and lamellipodia protrusions and in axon outgrowth. Binds to F-actin but not to RNA. Regulator of epithelial morphogenesis. May act as an invasion suppressor in cancers.
    • Sequence similarities

      Belongs to the CYFIP family.
    • Cellular localization

      Cytoplasm > perinuclear region. Cell projection > lamellipodium. Cell projection > ruffle. Cell junction > synapse > synaptosome. Highly expressed in the perinuclear region. Enriched in synaptosomes, membrane ruffles and at the tips of lamellipodia.
    • Information by UniProt
    • Database links

    • Alternative names

      • CY antibody
      • CYFIP 1 antibody
      • CYFIP1 antibody
      • CYFP1_HUMAN antibody
      • Cytoplasmic FMR1 interacting protein 1 antibody
      • Cytoplasmic FMR1-interacting protein 1 antibody
      • FLJ45151 antibody
      • KIAA0068 antibody
      • p140sra 1 antibody
      • p140sra-1 antibody
      • P140SRA1 antibody
      • Selective hybridizing clone antibody
      • SHYC antibody
      • Specifically Rac1 associated protein 1 antibody
      • Specifically Rac1-associated protein 1 antibody
      • SRA 1 antibody
      • Sra-1 antibody
      • SRA1 antibody
      see all

    Images

    • All lanes : Anti-CYFIP1 antibody [EPR10782] (ab156016) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : 293T cell lysate
      Lane 3 : A549 cell lysate
      Lane 4 : Fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 145 kDa

    • Immunofluorescent analysis of HeLa cells labeling CYFIP1 with ab156016 at 1/250 dilution.
    • Flow cytometric analysis of permeabilized 293T cells labeling CYFIP1 with ab156016 at 1/10 dilution (red) compared to a rabbit IgG negative control (green).

    References

    This product has been referenced in:

    • Bachmann SO  et al. Behavioral training rescues motor deficits in Cyfip1 haploinsufficiency mouse model of autism spectrum disorders. Transl Psychiatry 9:29 (2019). Read more (PubMed: 30664619) »
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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