Anti-CYFIP1 antibody - N-terminal (ab154045)

Overview

  • Product name

    Anti-CYFIP1 antibody - N-terminal
    See all CYFIP1 primary antibodies
  • Description

    Rabbit polyclonal to CYFIP1 - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-309 of Human CYFIP1 (Uniprot ID: Q7L576).

  • Positive control

    • HeLa and BCL1 whole cell lysates; HeLa cells; Human Hepatoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab154045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 145 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Component of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA (By similarity). Involved in formation of membrane ruffles and lamellipodia protrusions and in axon outgrowth. Binds to F-actin but not to RNA. Regulator of epithelial morphogenesis. May act as an invasion suppressor in cancers.
  • Sequence similarities

    Belongs to the CYFIP family.
  • Cellular localization

    Cytoplasm > perinuclear region. Cell projection > lamellipodium. Cell projection > ruffle. Cell junction > synapse > synaptosome. Highly expressed in the perinuclear region. Enriched in synaptosomes, membrane ruffles and at the tips of lamellipodia.
  • Information by UniProt
  • Database links

  • Alternative names

    • CY antibody
    • CYFIP 1 antibody
    • CYFIP1 antibody
    • CYFP1_HUMAN antibody
    • Cytoplasmic FMR1 interacting protein 1 antibody
    • Cytoplasmic FMR1-interacting protein 1 antibody
    • FLJ45151 antibody
    • KIAA0068 antibody
    • p140sra 1 antibody
    • p140sra-1 antibody
    • P140SRA1 antibody
    • Selective hybridizing clone antibody
    • SHYC antibody
    • Specifically Rac1 associated protein 1 antibody
    • Specifically Rac1-associated protein 1 antibody
    • SRA 1 antibody
    • Sra-1 antibody
    • SRA1 antibody
    see all

Images

  • Anti-CYFIP1 antibody - N-terminal (ab154045) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 145 kDa



    5% SDS PAGE
  • Anti-CYFIP1 antibody - N-terminal (ab154045) at 1/1000 dilution + BCL1 whole cell lysate at 30 µg

    Predicted band size: 145 kDa



    5% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells, labeling CYFIP1 with ab154045 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human Hepatoma tissue, labeling CYFIP1 with ab154045 at 1/500 dilution.

References

ab154045 has not yet been referenced specifically in any publications.

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