Key features and details
- Rabbit polyclonal to CYFIP1 - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CYFIP1 antibody - N-terminal
See all CYFIP1 primary antibodies
DescriptionRabbit polyclonal to CYFIP1 - N-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Zebrafish
Recombinant fragment, corresponding to a region within amino acids 1-309 of Human CYFIP1 (Uniprot ID: Q7L576).
- HeLa and BCL1 whole cell lysates; HeLa cells; Human Hepatoma tissue.
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We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab154045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 145 kDa.|
|IHC-P||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionComponent of the CYFIP1-EIF4E-FMR1 complex which binds to the mRNA cap and mediates translational repression. In the CYFIP1-EIF4E-FMR1 complex this subunit is an adapter between EIF4E and FMR1. Promotes the translation repression activity of FMR1 in brain probably by mediating its association with EIF4E and mRNA (By similarity). Involved in formation of membrane ruffles and lamellipodia protrusions and in axon outgrowth. Binds to F-actin but not to RNA. Regulator of epithelial morphogenesis. May act as an invasion suppressor in cancers.
Sequence similaritiesBelongs to the CYFIP family.
Cellular localizationCytoplasm > perinuclear region. Cell projection > lamellipodium. Cell projection > ruffle. Cell junction > synapse > synaptosome. Highly expressed in the perinuclear region. Enriched in synaptosomes, membrane ruffles and at the tips of lamellipodia.
- Information by UniProt
- CY antibody
- CYFIP 1 antibody
- CYFIP1 antibody
Anti-CYFIP1 antibody - N-terminal (ab154045) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 145 kDa
5% SDS PAGE
Anti-CYFIP1 antibody - N-terminal (ab154045) at 1/1000 dilution + BCL1 whole cell lysate at 30 µg
Predicted band size: 145 kDa
5% SDS PAGE
Immunofluorescent analysis of methanol-fixed HeLa cells, labeling CYFIP1 with ab154045 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
Immunohistochemical analysis of paraffin-embedded Human Hepatoma tissue, labeling CYFIP1 with ab154045 at 1/500 dilution.
ab154045 has not yet been referenced specifically in any publications.