Tested applicationsSuitable for: FM, ICC/IFmore details
CyGEL™ is a novel thermoreversible hydrogel which is liquid when cold and a gel when warmed at room temperature (22°C +/- 1°C). CyGEL™ is compatible with live cells and can be used to immobilize non-adherent cells by simple warming, and conversely, allowing their recovery by simple cooling.
CyGEL™ has many applications in imaging and cell-based screening.
Key features of CyGEL™ include:
- Convenient immobilization of non-adherent cells.
- Controllable and rapid transition from liquid to gel, which allows quick preparation of live cells for gel mounting.
- Optically clear with low autofluorescence.
- Compatible with GFP and other fluorescent probes such as DRAQ5™ (ab108410).
CyGEL™ is recommended for mounting live de-adherent/ non-adherent cells for microscopy, or mounting live C. elegans and parasites (e.g. leishmania, trypanosomes), which can be recovered afterwards. CyGEL™ is provided as concentrated non-buffered form. Add the concentrated PBS supplement (provided) to prime CyGEL™ prior use.
CyGEL™ is supplied as 8 vials, each containing 500µl, and 1 vial of 40xPBS.
For imaging experiments longer than 2 hours, we recommend using CyGEL Sustain™ (ab109205).
Storage instructionsStore at +4°C. Do not freeze.
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Our Abpromise guarantee covers the use of ab109204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|FM||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.
Please refer to the Protocol Booklet for detailed information about the use of CyGEL.
ab109204 has not yet been referenced specifically in any publications.