Overview

  • Product name
    CyGEL™
  • Tested applications
    Suitable for: FM, ICC/IFmore details
  • General notes

    CyGEL™ is a novel thermoreversible hydrogel which is liquid when cold and a gel when warmed at room temperature (22°C +/- 1°C). CyGEL™ is compatible with live cells and can be used to immobilize non-adherent cells by simple warming, and conversely, allowing their recovery by simple cooling.
    CyGEL™ has many applications in imaging and cell-based screening.
    Key features of CyGEL™ include:

    1. Convenient immobilization of non-adherent cells.
    2. Controllable and rapid transition from liquid to gel, which allows quick preparation of live cells for gel mounting.
    3. Optically clear with low autofluorescence.
    4. Compatible with GFP and other fluorescent probes such as DRAQ5™ (ab108410).


    CyGEL™ is recommended for mounting live de-adherent/ non-adherent cells for microscopy, or mounting live C. elegans and parasites (e.g. leishmania, trypanosomes), which can be recovered afterwards. CyGEL™ is provided as concentrated non-buffered form. Add the concentrated PBS supplement (provided) to prime CyGEL™ prior use.
    CyGEL™ is supplied as 8 vials, each containing 500µl, and 1 vial of 40xPBS.
    For imaging experiments longer than 2 hours, we recommend using CyGEL Sustain™ (ab109205).

Properties

Applications

Our Abpromise guarantee covers the use of ab109204 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
FM Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
Please refer to the Protocol Booklet for detailed information about the use of CyGEL.

Images

  • This ilustration demonstrates the general procedure for using CyGEL™ with live cells prior analysis by fluorescence microscopy.

Protocols

References

ab109204 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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excellent method

Excellent Excellent 5/5 (Ease of Use)
Abreviews
This is a good method to perform single cell analysis and afterwards to cultivate the cells again or to make additional testings.
It works well and reliably.
The instructions are easy to understand and easy to implement.
We have used it for immunofluorescence analysis.

Abcam user community

Verified customer

Submitted Oct 07 2015

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