Overview

  • Product name
    CyGEL Sustain™
  • Tested applications
    Suitable for: ICC/IF, FMmore details
  • General notes

    CyGEL Sustain™ is a novel thermoreversible hydrogel which is liquid when cold and a gel when warmed at room temperature (22°C +/- 1°C). CyGEL Sustain™ is compatible with live cells and can be used to immobilize non-adherent cells by simple warming, and conversely, allowing their recovery by simple cooling. CyGEL Sustain™ has many applications in imaging and cell-based screening.
    Key features of CyGEL Sustain™ include:

    • Convenient immobilization of non-adherent cells.
    • Controllable and rapid transition from liquid to gel, which allows quick perparation of live cells for gel mounting.
    • Optically clear with low autofluorescence.
    • Compatible with GFP and other fluorescent probes such as DRAQ5™ (ab108410).


    CyGEL Sustain™ is recommended for mounting live de-adherent/ non-adherent cells and Zebrafish embryos for microscopy. CyGEL Sustain™ is specially formulated to allow addition of RPMI and similar culture media, which makes it the best tool for extended imaging experiments, as cells will survive for several hours.
    CyGEL Sustain™ is supplied as 10 vials, each containing 500µl.
    For live C. elegans and parasites we recommend CyGEL™(ab109204).

Properties

Applications

Our Abpromise guarantee covers the use of ab109205 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.

Please refer to the Protocol Booklet for detailed information about the use of CyGEL Sustain.
FM Use at an assay dependent concentration.

Images

  • - CyGEL Sustain™ (ab109205)
    - CyGEL Sustain™ (ab109205)
    This ilustration demonstrates the general procedure for using CyGEL Sustain™ with live cells prior analysis by fluorescence microscopy.

Protocols

References

This product has been referenced in:
  • Fritzsche M  et al. Cytoskeletal actin dynamics shape a ramifying actin network underpinning immunological synapse formation. Sci Adv 3:e1603032 (2017). Read more (PubMed: 28691087) »

See 1 Publication for this product

Publishing research using ab109205? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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7 reviews or Q&A

10
Votes: Highest First

I am interested in using cygel (ab109204) for medium term (up to 20 hours) live imaging of insect embryos , and possibly as a way to immobilize them for injection. I saw that there have been sucessful uses of cygel for Drosophila, but only for shorter ...

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Thank you for contacting us.



As suggested, there is no particular reason to use CyGEL Sustain over CyGEL (a PBS-based formulation) other than the ease of adding specific media.

We have limited data on the long-term ima...

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Möchte ab109205 mindestens für 6 Stunden mit Insektenzellen benutzen. Länger ist besser. Was für Erfahrungswerte gibt es?

Vielen Dank für Ihren Anruf. Ich habe nun eine Antwort aus dem Labor erhalten. Leider haben wir nur Erfahrung mit humanen Zellen für bis zu 4 Stunden in CyGEL. Es wird allerding dringend empfohlen das Gel mit Medium und anderen benötigte...

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CyGEL sustain for suspension cells under the microscope

 Good Average 3/5 (Ease of Use)
Abreviews
The CyGEL sustain is a good gel to investigate suspension cells in an environment similar to the cell normal conditions. For microscopy purposes is a good approach since is optically inert. For the purposed I used, I employed a modified protocol than the provided one. This affects the time the gel last in it best conditions. Under these circumstances I could work with the gel for 3-4 hours without degradation. Noteworthy, one should carefully consider the properties before using it, as well as the environmental conditions where they plan the experiment. The osmolality needs to be considered, and for this purpose there is a special vial that comes along with the kit. But additionally, the temperature and humidity conditions, because the gel under dry conditions disintegrates faster. Therefore in physiologically relevant conditions the gel last less than what is specify in the brochure. The CyGEL sustain performs better under this conditions than the CyGEL.
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Verified customer

Submitted Mar 31 2015

Would you possibly be able to let me know whether CyGEL is synthesised artificially or generated from biological material? I do not need a detailed composition of the product, just this one point.



I can confirm that CyGEL (ab109205) is entirely synthetic and it contains no biologically-derived material.



Dear ,

I am interested to buy CyGel, I have trouble to enter in your web page. Can you send me please information about the product? I pretend to use it in Laser scanning confocal microscopy to work with cell culture at room temperature and ...

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Thank you for contacting us.

I am sorry you are not able to enter our Website, please do let us know if the problem persists. You will find attached the protocols for our CyGel products: ab109205 and ab109204.

These products should ...

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I am interested in performing a trial with CyGEL to immobilize suspension mammalian cells for time-lapse microscopy in a 96 well format. I did find the protocol PDF regarding the product but it only mentions that CuGEL can be used in a 96 well format. ...

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Thank you for contacting Abcam.

We do not have any data for this product being used in a 96-well format but that does not mean to say it will not work, just that we have not yet tested it in that format.

I am sorry that I am not abl...

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I received the wrong product (again), this time it was ab109204, instead of ab109205.

As requested, I have issued a free of charge replacement for one kit of ab109205. To check the status of the order please contact our Customer Service team and reference this number. Please note that this free of charge replacement vial is also covered...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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