Anti-CYLD antibody (ab137524)
Key features and details
- Rabbit polyclonal to CYLD
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CYLD antibody
See all CYLD primary antibodies -
Description
Rabbit polyclonal to CYLD -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Xenopus tropicalis -
Immunogen
Synthetic peptide corresponding to Human CYLD aa 896-906.
Sequence:GGQNGFNIPQV
Database link: Q9NQC7 -
Positive control
- ICC/IF: A431 cells. IHC-P: Human gastric carcinoma tissue. WB: HepG2 whole cell lysate. Rat brain lysate. C8D30, NIH-3T3 , BCL-1 , Raw264.7 , C2C12 whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab137524 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/500 - 1/3000. Predicted molecular weight: 107 kDa.
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IHC-P | (1) |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using
10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 107 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Has endodeubiquitinase activity. Plays an important role in the regulation of pathways leading to NF-kappa-B activation. Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. Negative regulator of Wnt signaling. Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. Plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. Required for normal cell cycle progress and normal cytokinesis. Inhibits nuclear translocation of NF-kappa-B. Plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. Dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. Negatively regulates TNFRSF11A signaling and osteoclastogenesis. -
Tissue specificity
Detected in fetal brain, testis, and skeletal muscle, and at a lower level in adult brain, leukocytes, liver, heart, kidney, spleen, ovary and lung. Isoform 2 is found in all tissues except kidney. -
Involvement in disease
Defects in CYLD are the cause of familial cylindromatosis (FCYL) [MIM:132700]; also known as Ancell-Spiegler cylindromas or turban tumor syndrome or dermal eccrine cylindromatosis. CYLD is an autosomal dominant and highly tumor type-specific disorder. The tumors (known as cylindromas because of their characteristic microscopic architecture) are believed to arise from or recapitulate the appearance of the eccrine or apocrine cells of the skin that secrete sweat and scent respectively. Cylindromas arise predominantly in hairy parts of the body with approximately 90% on the head and neck. The development of a confluent mass which may ulcerate or become infected has led to the designation 'turban tumor syndrome'. The skin tumors show differentiation in the direction of hair structures, hence the synonym trichoepithelioma.
Defects in CYLD are the cause of multiple familial trichoepithelioma type 1 (MFT1) [MIM:601606]; also known as epithelioma adenoides cysticum of Brooke (EAC) or hereditary multiple benign cystic epithelioma or Brooke-Fordyce trichoepitheliomas. MFT1 is an autosomal dominant dermatosis characterized by the presence of many skin tumors predominantly on the face. Since histologic examination shows dermal aggregates of basaloid cells with connection to or differentiation toward hair follicles, this disorder has been thought to represent a benign hamartoma of the pilosebaceous apparatus. Trichoepitheliomas can degenerate into basal cell carcinoma.
Defects in CYLD are the cause of Brooke-Spiegler syndrome (BRSS) [MIM:605041]. BRSS is an autosomal dominant disorder characterized by the appearance of multiple skin appendage tumors such as cylindroma, trichoepithelioma, and spiradenoma. These tumors are typically located in the head and neck region, appear in early adulthood, and gradually increase in size and number throughout life. -
Sequence similarities
Belongs to the peptidase C67 family.
Contains 3 CAP-Gly domains. -
Post-translational
modificationsPhosphorylated on several serine residues by IKKA and/or IKKB in response to immune stimuli. Phosphorylation requires IKBKG. Phosphorylation abolishes TRAF2 deubiquitination, interferes with the activation of Jun kinases, and strongly reduces CD40-dependent gene activation by NF-kappa-B. -
Cellular localization
Cytoplasm. Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton. Cell membrane. Detected at the microtubule cytoskeleton during interphase. Detected at the midbody during telophase. - Information by UniProt
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Database links
- Entrez Gene: 536421 Cow
- Entrez Gene: 1540 Human
- Entrez Gene: 74256 Mouse
- Entrez Gene: 312937 Rat
- Omim: 605018 Human
- SwissProt: Q1RMU2 Cow
- SwissProt: Q9NQC7 Human
- SwissProt: Q80TQ2 Mouse
see all -
Alternative names
- BRSS antibody
- CDMT antibody
- Cyld antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYLD antibody (ab137524)Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labelling CYLD with ab137524 at 1/100 dilution.
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Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labelling CYLD with ab137524 at 1/200 dilution. The lower image is merged with DNA probe.
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Anti-CYLD antibody (ab137524) at 1/500 dilution + Rat brain lysate at 50 µg
Predicted band size: 107 kDa7.5% SDS-PAGE
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Anti-CYLD antibody (ab137524) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 107 kDa
7.5% SDS PAGE -
All lanes : Anti-CYLD antibody (ab137524) at 1/1000 dilution
Lane 1 : C8D30 whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate
Lane 3 : BCL-1 whole cell lysate
Lane 4 : Raw264.7 whole cell lysate
Lane 5 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 107 kDa7.5% SDS-PAGE
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab137524 has been referenced in 6 publications.
- Chen Y & Yang C miR-197-3p-induced downregulation of lysine 63 deubiquitinase promotes cell proliferation and inhibits cell apoptosis in lung adenocarcinoma cell lines. Mol Med Rep 17:3921-3927 (2018). PubMed: 29286108
- Liu H et al. Alterations of 63 hub genes during lingual carcinogenesis in C57BL/6J mice. Sci Rep 8:12626 (2018). PubMed: 30135512
- Zhang L et al. The deubiquitinase CYLD is a specific checkpoint of the STING antiviral signaling pathway. PLoS Pathog 14:e1007435 (2018). PubMed: 30388174
- Rito M et al. Frequent and differential mutations of the CYLD gene in basal cell salivary neoplasms: linkage to tumor development and progression. Mod Pathol 31:1064-1072 (2018). PubMed: 29463883
- Hong L et al. Expression of the deubiquitinase cylindromatosis in articular cartilage and subchondral bone is associated with the severity of knee osteoarthritis. Exp Ther Med 14:5990-5996 (2017). PubMed: 29285147
- Welte S et al. Nuclear expression of the deubiquitinase CYLD is associated with improved survival in human hepatocellular carcinoma. PLoS One 9:e110591 (2014). IHC ; Human . PubMed: 25329885