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    cyld-antibody-ab137524.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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Anti-CYLD antibody (ab137524)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (6)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYLD antibody (ab137524)
  • Immunocytochemistry/ Immunofluorescence - Anti-CYLD antibody (ab137524)
  • Western blot - Anti-CYLD antibody (ab137524)
  • Western blot - Anti-CYLD antibody (ab137524)
  • Western blot - Anti-CYLD antibody (ab137524)

Key features and details

  • Rabbit polyclonal to CYLD
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CYLD antibody
    See all CYLD primary antibodies
  • Description

    Rabbit polyclonal to CYLD
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow, Xenopus tropicalis
  • Immunogen

    Synthetic peptide corresponding to Human CYLD aa 896-906.
    Sequence:

    GGQNGFNIPQV


    Database link: Q9NQC7
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • ICC/IF: A431 cells. IHC-P: Human gastric carcinoma tissue. WB: HepG2 whole cell lysate. Rat brain lysate. C8D30, NIH-3T3 , BCL-1 , Raw264.7 , C2C12 whole cell lysate.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Cell Biology
    • Proteolysis / Ubiquitin
    • Proteasome / Ubiquitin
    • Deubiquitination
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Epigenetics and Nuclear Signaling
    • Ubiquitin & Ubiquitin Like Modifiers
    • Deubiquitination

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Hep G2 cytoplasmic extract lysate (ab14659)
    • HeLa whole cell lysate (ab29545)
    • HeLa membrane extract lysate (ab29547)
    • Raji whole cell lysate (ab30124)
    • Raji membrane extract lysate (ab30126)
    • A-431 whole cell lysate (ab30132)
    • A-431 membrane extract lysate (ab30134)
    • A-431 whole cell lysate (ab7909)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab137524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (1)
1/500 - 1/3000. Predicted molecular weight: 107 kDa.
IHC-P (1)
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF
1/100 - 1/1000.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 107 kDa.
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF
1/100 - 1/1000.

Target

  • Function

    Protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Has endodeubiquitinase activity. Plays an important role in the regulation of pathways leading to NF-kappa-B activation. Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. Negative regulator of Wnt signaling. Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. Plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. Required for normal cell cycle progress and normal cytokinesis. Inhibits nuclear translocation of NF-kappa-B. Plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. Dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. Negatively regulates TNFRSF11A signaling and osteoclastogenesis.
  • Tissue specificity

    Detected in fetal brain, testis, and skeletal muscle, and at a lower level in adult brain, leukocytes, liver, heart, kidney, spleen, ovary and lung. Isoform 2 is found in all tissues except kidney.
  • Involvement in disease

    Defects in CYLD are the cause of familial cylindromatosis (FCYL) [MIM:132700]; also known as Ancell-Spiegler cylindromas or turban tumor syndrome or dermal eccrine cylindromatosis. CYLD is an autosomal dominant and highly tumor type-specific disorder. The tumors (known as cylindromas because of their characteristic microscopic architecture) are believed to arise from or recapitulate the appearance of the eccrine or apocrine cells of the skin that secrete sweat and scent respectively. Cylindromas arise predominantly in hairy parts of the body with approximately 90% on the head and neck. The development of a confluent mass which may ulcerate or become infected has led to the designation 'turban tumor syndrome'. The skin tumors show differentiation in the direction of hair structures, hence the synonym trichoepithelioma.
    Defects in CYLD are the cause of multiple familial trichoepithelioma type 1 (MFT1) [MIM:601606]; also known as epithelioma adenoides cysticum of Brooke (EAC) or hereditary multiple benign cystic epithelioma or Brooke-Fordyce trichoepitheliomas. MFT1 is an autosomal dominant dermatosis characterized by the presence of many skin tumors predominantly on the face. Since histologic examination shows dermal aggregates of basaloid cells with connection to or differentiation toward hair follicles, this disorder has been thought to represent a benign hamartoma of the pilosebaceous apparatus. Trichoepitheliomas can degenerate into basal cell carcinoma.
    Defects in CYLD are the cause of Brooke-Spiegler syndrome (BRSS) [MIM:605041]. BRSS is an autosomal dominant disorder characterized by the appearance of multiple skin appendage tumors such as cylindroma, trichoepithelioma, and spiradenoma. These tumors are typically located in the head and neck region, appear in early adulthood, and gradually increase in size and number throughout life.
  • Sequence similarities

    Belongs to the peptidase C67 family.
    Contains 3 CAP-Gly domains.
  • Post-translational
    modifications

    Phosphorylated on several serine residues by IKKA and/or IKKB in response to immune stimuli. Phosphorylation requires IKBKG. Phosphorylation abolishes TRAF2 deubiquitination, interferes with the activation of Jun kinases, and strongly reduces CD40-dependent gene activation by NF-kappa-B.
  • Cellular localization

    Cytoplasm. Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton. Cell membrane. Detected at the microtubule cytoskeleton during interphase. Detected at the midbody during telophase.
  • Target information above from: UniProt accession Q9NQC7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 536421 Cow
    • Entrez Gene: 1540 Human
    • Entrez Gene: 74256 Mouse
    • Entrez Gene: 312937 Rat
    • Omim: 605018 Human
    • SwissProt: Q1RMU2 Cow
    • SwissProt: Q9NQC7 Human
    • SwissProt: Q80TQ2 Mouse
    • SwissProt: Q66H62 Rat
    • Unigene: 578973 Human
    • Unigene: 482446 Mouse
    • Unigene: 128760 Rat
    • Unigene: 168938 Rat
    see all
  • Alternative names

    • BRSS antibody
    • CDMT antibody
    • Cyld antibody
    • CYLD gene antibody
    • CYLD_HUMAN antibody
    • CYLD1 antibody
    • CYLDI antibody
    • cylindromatosis (turban tumor syndrome) antibody
    • cylindromatosis 1 antibody
    • Deubiquitinating enzyme CYLD antibody
    • EAC antibody
    • HSPC057 antibody
    • KIAA0849 antibody
    • MFT antibody
    • MFT1 antibody
    • Probable ubiquitin carboxyl terminal hydrolase CYLD antibody
    • SBS antibody
    • TEM antibody
    • turban tumor syndrome antibody
    • Ubiquitin carboxyl-terminal hydrolase CYLD antibody
    • ubiquitin specific peptidase like 2 antibody
    • ubiquitin thioesterase CYLD antibody
    • Ubiquitin thiolesterase CYLD antibody
    • Ubiquitin-specific processing protease CYLD antibody
    • Ubiquitin-specific-processing protease CYLD antibody
    • USPL2 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYLD antibody (ab137524)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYLD antibody (ab137524)
    Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labelling CYLD with ab137524 at 1/100 dilution.
  • Immunocytochemistry/ Immunofluorescence - Anti-CYLD antibody (ab137524)
    Immunocytochemistry/ Immunofluorescence - Anti-CYLD antibody (ab137524)
    Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labelling CYLD with ab137524 at 1/200 dilution. The lower image is merged with DNA probe.
  • Western blot - Anti-CYLD antibody (ab137524)
    Western blot - Anti-CYLD antibody (ab137524)
    Anti-CYLD antibody (ab137524) at 1/500 dilution + Rat brain lysate at 50 µg

    Predicted band size: 107 kDa



    7.5% SDS-PAGE

  • Western blot - Anti-CYLD antibody (ab137524)
    Western blot - Anti-CYLD antibody (ab137524)
    Anti-CYLD antibody (ab137524) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 107 kDa



    7.5% SDS PAGE
  • Western blot - Anti-CYLD antibody (ab137524)
    Western blot - Anti-CYLD antibody (ab137524)
    All lanes : Anti-CYLD antibody (ab137524) at 1/1000 dilution

    Lane 1 : C8D30 whole cell lysate
    Lane 2 : NIH/3T3 whole cell lysate
    Lane 3 : BCL-1 whole cell lysate
    Lane 4 : Raw264.7 whole cell lysate
    Lane 5 : C2C12 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 107 kDa



    7.5% SDS-PAGE

Protocols

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (6)

Publishing research using ab137524? Please let us know so that we can cite the reference in this datasheet.

ab137524 has been referenced in 6 publications.

  • Chen Y & Yang C miR-197-3p-induced downregulation of lysine 63 deubiquitinase promotes cell proliferation and inhibits cell apoptosis in lung adenocarcinoma cell lines. Mol Med Rep 17:3921-3927 (2018). PubMed: 29286108
  • Liu H  et al. Alterations of 63 hub genes during lingual carcinogenesis in C57BL/6J mice. Sci Rep 8:12626 (2018). PubMed: 30135512
  • Zhang L  et al. The deubiquitinase CYLD is a specific checkpoint of the STING antiviral signaling pathway. PLoS Pathog 14:e1007435 (2018). PubMed: 30388174
  • Rito M  et al. Frequent and differential mutations of the CYLD gene in basal cell salivary neoplasms: linkage to tumor development and progression. Mod Pathol 31:1064-1072 (2018). PubMed: 29463883
  • Hong L  et al. Expression of the deubiquitinase cylindromatosis in articular cartilage and subchondral bone is associated with the severity of knee osteoarthritis. Exp Ther Med 14:5990-5996 (2017). PubMed: 29285147
  • Welte S  et al. Nuclear expression of the deubiquitinase CYLD is associated with improved survival in human hepatocellular carcinoma. PLoS One 9:e110591 (2014). IHC ; Human . PubMed: 25329885

Customer reviews and Q&As

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1-2 of 2 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CYLD antibody

Average
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer (pH8.0)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde
Read More

Dr. Konstantinos Xanthopoulos

Verified customer

Submitted Jan 30 2015

Western blot abreview for Anti-CYLD antibody

Average
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Mouse Cell lysate - whole cell (N2a)
Specification
N2a
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Read More

Abcam user community

Verified customer

Submitted Jan 30 2015

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