Overview

  • Product name
  • Description
    Rabbit polyclonal to CYLD
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Xenopus tropicalis
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 896-906 of Human CYLD (Q9NQC7).

  • Positive control
    • 293T, H1299, HeLa, HepG2, A431 and Raji whole cell lysates; A431 cells; Human gastric carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab137524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 107 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

  • Function
    Protease that specifically cleaves 'Lys-63'-linked polyubiquitin chains. Has endodeubiquitinase activity. Plays an important role in the regulation of pathways leading to NF-kappa-B activation. Contributes to the regulation of cell survival, proliferation and differentiation via its effects on NF-kappa-B activation. Negative regulator of Wnt signaling. Inhibits HDAC6 and thereby promotes acetylation of alpha-tubulin and stabilization of microtubules. Plays a role in the regulation of microtubule dynamics, and thereby contributes to the regulation of cell proliferation, cell polarization, cell migration, and angiogenesis. Required for normal cell cycle progress and normal cytokinesis. Inhibits nuclear translocation of NF-kappa-B. Plays a role in the regulation of inflammation and the innate immune response, via its effects on NF-kappa-B activation. Dispensable for the maturation of intrathymic natural killer cells, but required for the continued survival of immature natural killer cells. Negatively regulates TNFRSF11A signaling and osteoclastogenesis.
  • Tissue specificity
    Detected in fetal brain, testis, and skeletal muscle, and at a lower level in adult brain, leukocytes, liver, heart, kidney, spleen, ovary and lung. Isoform 2 is found in all tissues except kidney.
  • Involvement in disease
    Defects in CYLD are the cause of familial cylindromatosis (FCYL) [MIM:132700]; also known as Ancell-Spiegler cylindromas or turban tumor syndrome or dermal eccrine cylindromatosis. CYLD is an autosomal dominant and highly tumor type-specific disorder. The tumors (known as cylindromas because of their characteristic microscopic architecture) are believed to arise from or recapitulate the appearance of the eccrine or apocrine cells of the skin that secrete sweat and scent respectively. Cylindromas arise predominantly in hairy parts of the body with approximately 90% on the head and neck. The development of a confluent mass which may ulcerate or become infected has led to the designation 'turban tumor syndrome'. The skin tumors show differentiation in the direction of hair structures, hence the synonym trichoepithelioma.
    Defects in CYLD are the cause of multiple familial trichoepithelioma type 1 (MFT1) [MIM:601606]; also known as epithelioma adenoides cysticum of Brooke (EAC) or hereditary multiple benign cystic epithelioma or Brooke-Fordyce trichoepitheliomas. MFT1 is an autosomal dominant dermatosis characterized by the presence of many skin tumors predominantly on the face. Since histologic examination shows dermal aggregates of basaloid cells with connection to or differentiation toward hair follicles, this disorder has been thought to represent a benign hamartoma of the pilosebaceous apparatus. Trichoepitheliomas can degenerate into basal cell carcinoma.
    Defects in CYLD are the cause of Brooke-Spiegler syndrome (BRSS) [MIM:605041]. BRSS is an autosomal dominant disorder characterized by the appearance of multiple skin appendage tumors such as cylindroma, trichoepithelioma, and spiradenoma. These tumors are typically located in the head and neck region, appear in early adulthood, and gradually increase in size and number throughout life.
  • Sequence similarities
    Belongs to the peptidase C67 family.
    Contains 3 CAP-Gly domains.
  • Post-translational
    modifications
    Phosphorylated on several serine residues by IKKA and/or IKKB in response to immune stimuli. Phosphorylation requires IKBKG. Phosphorylation abolishes TRAF2 deubiquitination, interferes with the activation of Jun kinases, and strongly reduces CD40-dependent gene activation by NF-kappa-B.
  • Cellular localization
    Cytoplasm. Cytoplasm > perinuclear region. Cytoplasm > cytoskeleton. Cell membrane. Detected at the microtubule cytoskeleton during interphase. Detected at the midbody during telophase.
  • Information by UniProt
  • Database links
  • Alternative names
    • BRSS antibody
    • CDMT antibody
    • Cyld antibody
    • CYLD gene antibody
    • CYLD_HUMAN antibody
    • CYLD1 antibody
    • CYLDI antibody
    • cylindromatosis (turban tumor syndrome) antibody
    • cylindromatosis 1 antibody
    • Deubiquitinating enzyme CYLD antibody
    • EAC antibody
    • HSPC057 antibody
    • KIAA0849 antibody
    • MFT antibody
    • MFT1 antibody
    • Probable ubiquitin carboxyl terminal hydrolase CYLD antibody
    • SBS antibody
    • TEM antibody
    • turban tumor syndrome antibody
    • Ubiquitin carboxyl-terminal hydrolase CYLD antibody
    • ubiquitin specific peptidase like 2 antibody
    • ubiquitin thioesterase CYLD antibody
    • Ubiquitin thiolesterase CYLD antibody
    • Ubiquitin-specific processing protease CYLD antibody
    • Ubiquitin-specific-processing protease CYLD antibody
    • USPL2 antibody
    see all

Images

  • Anti-CYLD antibody (ab137524) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 107 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin embedded Human gastric carcinoma tissue labelling CYLD with ab137524 at 1/100 dilution.
  • Immunofluorescent analysis of paraformaldehyde-fixed A431 cells labelling CYLD with ab137524 at 1/200 dilution. The lower image is merged with DNA probe.

References

This product has been referenced in:
  • Chen Y & Yang C miR-197-3p-induced downregulation of lysine 63 deubiquitinase promotes cell proliferation and inhibits cell apoptosis in lung adenocarcinoma cell lines. Mol Med Rep 17:3921-3927 (2018). Read more (PubMed: 29286108) »
  • Liu H  et al. Alterations of 63 hub genes during lingual carcinogenesis in C57BL/6J mice. Sci Rep 8:12626 (2018). Read more (PubMed: 30135512) »
See all 5 Publications for this product

Customer reviews and Q&As

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1-2 of 2 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 22°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Tris-EDTA buffer (pH8.0)
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Permeabilization
No
Fixative
Paraformaldehyde

Dr. Konstantinos Xanthopoulos

Verified customer

Submitted Jan 30 2015

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Mouse Cell lysate - whole cell (N2a)
Specification
N2a
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Jan 30 2015

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