Overview

  • Product name

  • Description

    Mouse polyclonal to CYP11A1
  • Host species

    Mouse
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-521 of Human CYP11A1

  • Positive control

    • CYP11A1 transfected 293T cell lysate.

Applications

Our Abpromise guarantee covers the use of ab67355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: 1/500 - 1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 60 kDa).
    Detection Pair.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.
    • Pathway

      Lipid metabolism; C21-steroid hormone metabolism.
    • Involvement in disease

      Defects in CYP11A1 are a cause of congenital adrenal insufficiency (CAI).
      Defects in CYP11A1 are a cause of congenital lipoid adrenal hyperplasia (CLAH) [MIM:201710]; also known as lipoid CAH. CLAH is the most severe form of adrenal hyperplasia. This autosomal recessive and potentially lethal condition includes the onset of profound adrenocortical insufficiency shortly after birth, hyperpigmentation reflecting increased production of pro-opiomelanocortin, elevated plasma renin activity as a consequence of reduced aldosterone synthesis, and male pseudohermaphroditism resulting from deficient fetal testicular testosterone synthesis. CLAH is a rare disease, except in Japan and Korea where it accounts for a significant percentage of cases of congenital adrenal hyperplasia.
    • Sequence similarities

      Belongs to the cytochrome P450 family.
    • Cellular localization

      Mitochondrion membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cholesterol 20 22 desmolase antibody
      • Cholesterol desmolase antibody
      • Cholesterol monooxygenase (side chain cleaving) antibody
      • Cholesterol side chain cleavage enzyme antibody
      • Cholesterol side chain cleavage enzyme mitochondrial antibody
      • Cholesterol side-chain cleavage enzyme antibody
      • CP11A_HUMAN antibody
      • CYP11A antibody
      • CYP11A1 antibody
      • CYPXIA1 antibody
      • Cytochrome P450 11A1 antibody
      • Cytochrome P450 11A1 mitochondrial antibody
      • Cytochrome P450 family 11 subfamily A polypeptide 1 antibody
      • Cytochrome P450 subfamily XIA antibody
      • Cytochrome P450(scc) antibody
      • Cytochrome P450C11A1 antibody
      • mitochondrial antibody
      • P450SCC antibody
      • Steroid 20 22 lyase antibody
      see all

    Images

    • All lanes : Anti-CYP11A1 antibody (ab67355) at 1/500 dilution

      Lane 1 : CYP11A1 transfected 293T cell lysate
      Lane 2 : Non transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Secondary
      All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

      Predicted band size: 60 kDa
      Observed band size: 60 kDa
      Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.

    References

    This product has been referenced in:

    • Aumo L  et al. Functional roles of protein kinase A (PKA) and exchange protein directly activated by 3',5'-cyclic adenosine 5'-monophosphate (cAMP) 2 (EPAC2) in cAMP-mediated actions in adrenocortical cells. Endocrinology 151:2151-61 (2010). WB ; Human, Mouse . Read more (PubMed: 20233795) »
    See 1 Publication for this product

    Customer reviews and Q&As

    Answer

    Thank you for your enquiry and your interest in our products. I have conducted a search for you and found some antibodies against CYP11A1. I am not sure if the host and the target species is important for your customer. These are all raised against human CYP11A1 and not rat. -ab75497: immunogen is commercially sensitive and not available, -ab67355: full length protein, corresponding to amino acids 1-521, -ab86357: synthetic peptide from within residues 400 - 500, -ab79820: immunogen is synthetic peptide selected from the N terminal region If you need any further assistance in the future, please do not hesitate to contact me.

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