Overview

  • Product name

  • Description

    Rabbit polyclonal to CYP1A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Dog, Pig, Chimpanzee, Macaque monkey, Chinese hamster, Orangutan
  • Immunogen

    Synthetic peptide within Human CYP1A1 aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    (Peptide available as ab155750)

  • Positive control

    • This antibody gave a positive signal in both Human Lung and Placenta tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab124295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 58 kDa).

Target

  • Function

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
  • Tissue specificity

    Lung, lymphocytes and placenta.
  • Sequence similarities

    Belongs to the cytochrome P450 family.
  • Cellular localization

    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AHH antibody
    • AHRR antibody
    • Aryl hydrocarbon hydroxylase antibody
    • CP11 antibody
    • CP1A1_HUMAN antibody
    • CYP 1 antibody
    • CYP1 antibody
    • cyp1a1 antibody
    • CYPIA1 antibody
    • Cytochrome P1 450 dioxin inducible antibody
    • Cytochrome P1-450 antibody
    • Cytochrome P450 1A1 antibody
    • Cytochrome P450 family 1 subfamily A polypeptide 1 antibody
    • Cytochrome P450 form 6 antibody
    • Cytochrome P450 subfamily I (aromatic compound inducible) polypeptide 1 antibody
    • Cytochrome P450-C antibody
    • Cytochrome P450-P1 antibody
    • Flavoprotein-linked monooxygenase antibody
    • Microsomal monooxygenase antibody
    • P1 450 antibody
    • P450 C antibody
    • P450 form 6 antibody
    • P450 P1 antibody
    • P450DX antibody
    • Xenobiotic monooxygenase antibody
    see all

Images

  • All lanes : Anti-CYP1A1 antibody (ab124295) at 1 µg/ml

    Lane 1 : Lung (Human) Tissue Lysate
    Lane 2 : Human placenta tissue lysate - total protein (ab29745)
    Lane 3 : Lung (Human) Tissue Lysate with Immunising peptide at 1 µg/ml
    Lane 4 : Human placenta tissue lysate - total protein (ab29745) with Immunising peptide at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 73 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 48 kDa, 55 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 90 seconds


    This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124295 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab124295 has not yet been referenced specifically in any publications.

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