Key features and details
- Rabbit polyclonal to CYP1A1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CYP1A1 antibody
See all CYP1A1 primary antibodies
DescriptionRabbit polyclonal to CYP1A1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Dog, Pig, Chimpanzee, Macaque monkey, Chinese hamster, Orangutan
Synthetic peptide within Human CYP1A1 aa 50-150 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
(Peptide available as
- This antibody gave a positive signal in both Human Lung and Placenta tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
Our Abpromise guarantee covers the use of ab124295 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 58 kDa).|
FunctionCytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Tissue specificityLung, lymphocytes and placenta.
Sequence similaritiesBelongs to the cytochrome P450 family.
Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
- Information by UniProt
- AHH antibody
- AHRR antibody
- Aryl hydrocarbon hydroxylase antibody
All lanes : Anti-CYP1A1 antibody (ab124295) at 1 µg/ml
Lane 1 : Lung (Human) Tissue Lysate
Lane 2 : Human placenta tissue lysate - total protein (ab29745)
Lane 3 : Lung (Human) Tissue Lysate with Immunising peptide at 1 µg/ml
Lane 4 : Human placenta tissue lysate - total protein (ab29745) with Immunising peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
Additional bands at: 48 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 90 seconds
This blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab124295 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab124295 has not yet been referenced specifically in any publications.