Product nameAnti-CYP1A1 antibody
See all CYP1A1 primary antibodies
DescriptionRabbit polyclonal to CYP1A1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide. This information is considered to be commercially sensitive.
- ICC/IF: HepG2 cells. IHC-P: Human kidney tissue. WB: CYP1A1 overexpressing lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
Our Abpromise guarantee covers the use of ab235185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 15 µg/ml.
Incubate with primary antibody overnight at 4°C.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate with primary antibody for 30 minutes at RT.
|WB||1/1000. Predicted molecular weight: 58 kDa.|
FunctionCytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Tissue specificityLung, lymphocytes and placenta.
Sequence similaritiesBelongs to the cytochrome P450 family.
Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
- Information by UniProt
- AHH antibody
- AHRR antibody
- Aryl hydrocarbon hydroxylase antibody
All lanes : Anti-CYP1A1 antibody (ab235185) at 1/1000 dilution
Lane 1 : CYP1A1 overexpressing lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell line) lysate
All lanes : Rabbit IgG (H&L) secondary antibody peroxidase conjugated Pre-adsorbed at 1/70000 dilution
Predicted band size: 58 kDa
Exposure time: 5 seconds
Blocking/Dilution buffer: Proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerisol added as an anti-microbial agent.
Formalin/PFA-fixed paraffin-embedded human kidney tissue stained for CYP1A1 with ab235185 at a 1/100 dilution in immunohistochemical analysis. Secondary antibody is an anti-Rabbit poly-HRP-IgG.
Incubated with primary antibody for 30 minutes at room temperature, followed by DAB staining.
Counterstained with hematoxylin. HIER using citrate buffer for 20 minutes.
A. Human kidney tissue counterstained with Hematoxilyn-Eosin.
B. Human kidney tissue stained for Cytochrome P450 1A1 (20X).
C. Human kidney tissue stained for Cytochrome P450 1A1 (40X).
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CYP1A1 using ab235185 at 15 µg/ml in ICC/IF.
Cells were fixed in 4% PFA and permeabilized using 0.3% Triton X-100, then incubated with the primary antibody overnight at 4°C. Secondary antibody is a Donkey anti-Rabbit IgG DyLight™ 488 (Preadsorbed), at 5 µg/ml for 1 hour at room temperature.
Panel A: DAPI (blue)
Panel B: ab235185 + secondary antibody (green)
Panel C: Actin-Phalloidin 555 (red)
Panel D: Secondary antibody only
Panel E: Merge A+B
Panel F: Merge A+B+C
ab235185 has not yet been referenced specifically in any publications.