Overview

  • Product name

  • Description

    Rabbit polyclonal to CYP1A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • ICC/IF: HepG2 cells. IHC-P: Human kidney tissue. WB: CYP1A1 overexpressing lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab235185 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 15 µg/ml.

Incubate with primary antibody overnight at 4°C.

IHC-P 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Incubate with primary antibody for 30 minutes at RT.

WB 1/1000. Predicted molecular weight: 58 kDa.

Target

  • Function

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
  • Tissue specificity

    Lung, lymphocytes and placenta.
  • Sequence similarities

    Belongs to the cytochrome P450 family.
  • Cellular localization

    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • AHH antibody
    • AHRR antibody
    • Aryl hydrocarbon hydroxylase antibody
    • CP11 antibody
    • CP1A1_HUMAN antibody
    • CYP 1 antibody
    • CYP1 antibody
    • cyp1a1 antibody
    • CYPIA1 antibody
    • Cytochrome P1 450 dioxin inducible antibody
    • Cytochrome P1-450 antibody
    • Cytochrome P450 1A1 antibody
    • Cytochrome P450 family 1 subfamily A polypeptide 1 antibody
    • Cytochrome P450 form 6 antibody
    • Cytochrome P450 subfamily I (aromatic compound inducible) polypeptide 1 antibody
    • Cytochrome P450-C antibody
    • Cytochrome P450-P1 antibody
    • Flavoprotein-linked monooxygenase antibody
    • Microsomal monooxygenase antibody
    • P1 450 antibody
    • P450 C antibody
    • P450 form 6 antibody
    • P450 P1 antibody
    • P450DX antibody
    • Xenobiotic monooxygenase antibody
    see all

Images

  • All lanes : Anti-CYP1A1 antibody (ab235185) at 1/1000 dilution

    Lane 1 : CYP1A1 overexpressing lysate
    Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
    Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell line) lysate

    Secondary
    All lanes : Rabbit IgG (H&L) secondary antibody peroxidase conjugated Pre-adsorbed at 1/70000 dilution

    Predicted band size: 58 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: Proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerisol added as an anti-microbial agent.

  • Formalin/PFA-fixed paraffin-embedded human kidney tissue stained for CYP1A1 with ab235185 at a 1/100 dilution in immunohistochemical analysis. Secondary antibody is an anti-Rabbit poly-HRP-IgG.

    Incubated with primary antibody for 30 minutes at room temperature, followed by DAB staining.

    Counterstained with hematoxylin. HIER using citrate buffer for 20 minutes.

    A. Human kidney tissue counterstained with Hematoxilyn-Eosin.

    B. Human kidney tissue stained for Cytochrome P450 1A1 (20X).

    C. Human kidney tissue stained for Cytochrome P450 1A1 (40X).

     

  • HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CYP1A1 using ab235185 at 15 µg/ml in ICC/IF.

    Cells were fixed in 4% PFA and permeabilized using 0.3% Triton X-100, then incubated with the primary antibody overnight at 4°C. Secondary antibody is a Donkey anti-Rabbit IgG DyLight™ 488 (Preadsorbed), at 5 µg/ml for 1 hour at room temperature.

    Panel A: DAPI (blue)         

    Panel B: ab235185 + secondary antibody (green)         

    Panel C: Actin-Phalloidin 555 (red)

    Panel D: Secondary antibody only                  

    Panel E: Merge A+B

    Panel F: Merge A+B+C

References

ab235185 has not yet been referenced specifically in any publications.

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