Key features and details
- Rabbit polyclonal to CYP1A1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CYP1A1 antibody
See all CYP1A1 primary antibodies
DescriptionRabbit polyclonal to CYP1A1
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Human
Synthetic peptide. This information is considered to be commercially sensitive.
- ICC/IF: HepG2 cells. IHC-P: Human kidney tissue. WB: CYP1A1 overexpressing lysate.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Concentration information loading...
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
Our Abpromise guarantee covers the use of ab235185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 15 µg/ml.
Incubate with primary antibody overnight at 4°C.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Incubate with primary antibody for 30 minutes at RT.
|WB||1/1000. Predicted molecular weight: 58 kDa.|
FunctionCytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Tissue specificityLung, lymphocytes and placenta.
Sequence similaritiesBelongs to the cytochrome P450 family.
Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
- Information by UniProt
- AHH antibody
- AHRR antibody
- Aryl hydrocarbon hydroxylase antibody
All lanes : Anti-CYP1A1 antibody (ab235185) at 1/1000 dilution
Lane 1 : CYP1A1 overexpressing lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow) lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma cell line) lysate
All lanes : Rabbit IgG (H&L) secondary antibody peroxidase conjugated Pre-adsorbed at 1/70000 dilution
Predicted band size: 58 kDa
Exposure time: 5 seconds
Blocking/Dilution buffer: Proprietary protein formulation in TRIS buffered saline at pH 7.6 with thimerisol added as an anti-microbial agent.
Formalin/PFA-fixed paraffin-embedded human kidney tissue stained for CYP1A1 with ab235185 at a 1/100 dilution in immunohistochemical analysis. Secondary antibody is an anti-Rabbit poly-HRP-IgG.
Incubated with primary antibody for 30 minutes at room temperature, followed by DAB staining.
Counterstained with hematoxylin. HIER using citrate buffer for 20 minutes.
A. Human kidney tissue counterstained with Hematoxilyn-Eosin.
B. Human kidney tissue stained for Cytochrome P450 1A1 (20X).
C. Human kidney tissue stained for Cytochrome P450 1A1 (40X).
HepG2 (human liver hepatocellular carcinoma cell line) cells stained for CYP1A1 using ab235185 at 15 µg/ml in ICC/IF.
Cells were fixed in 4% PFA and permeabilized using 0.3% Triton X-100, then incubated with the primary antibody overnight at 4°C. Secondary antibody is a Donkey anti-Rabbit IgG DyLight™ 488 (Preadsorbed), at 5 µg/ml for 1 hour at room temperature.
Panel A: DAPI (blue)
Panel B: ab235185 + secondary antibody (green)
Panel C: Actin-Phalloidin 555 (red)
Panel D: Secondary antibody only
Panel E: Merge A+B
Panel F: Merge A+B+C
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab235185 has not yet been referenced specifically in any publications.