Overview

  • Product name
    Anti-CYP1B1 antibody [EPR14972] - BSA and Azide free
    See all CYP1B1 primary antibodies
  • Description
    Rabbit monoclonal [EPR14972] to CYP1B1 - BSA and Azide free
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human CYP1B1 aa 400 to the C-terminus (C terminal). The exact sequence is proprietary.
    Database link: Q16678

  • Positive control
    • IHC-P: Human renal adenocarcinoma tissue.
  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®). 

    ab232645 is a PBS-only buffer format of ab185954. Please refer to ab185954 for recommended dilutions, protocols, and image data.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab232645 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function
      Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
      Participates in the metabolism of an as-yet-unknown biologically active molecule that is a participant in eye development.
    • Tissue specificity
      Expressed in many tissues.
    • Involvement in disease
      Defects in CYP1B1 are the cause of primary congenital glaucoma type 3A (GLC3A) [MIM:231300]. GLC3A is an autosomal recessive form of primary congenital glaucoma (PCG). PCG is characterized by marked increase of intraocular pressure at birth or early childhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor.
      Defects in CYP1B1 are a cause of primary open angle glaucoma (POAG) [MIM:137760]. POAG is a complex and genetically heterogeneous ocular disorder characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. In some cases, POAG shows digenic inheritance involving mutations in CYP1B1 and MYOC genes.
      Defects in CYP1B1 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly is a congenital defect of the anterior chamber of the eye.
    • Sequence similarities
      Belongs to the cytochrome P450 family.
    • Cellular localization
      Endoplasmic reticulum membrane. Microsome membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • Aryl hydrocarbon hydroxylase antibody
      • CP1B antibody
      • CP1B1_HUMAN antibody
      • Cyp1b1 antibody
      • CYPIB1 antibody
      • Cytochrome P450 1B1 antibody
      • Cytochrome P450 family 1 subfamily B polypeptide 1 antibody
      • Cytochrome P450 subfamily I (dioxin inducible) polypeptide 1 (glaucoma 3 primary infantile) antibody
      • Flavoprotein linked monooxygenase antibody
      • GLC3A antibody
      • Microsomal monooxygenase antibody
      • P4501B1 antibody
      • Xenobiotic monooxygenase antibody
      see all

    Images

    • Immunohistochemical analysis of paraffin-embedded human renal adenocarcinoma tissue labeling CYP1B1 with ab185954 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab185954).

    References

    ab232645 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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