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    cyp1b1-antibody-epr14972-c-terminal-ab185954.pdf

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RecombinantRabMAb

Recombinant Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)

  • Datasheet
  • SDS
  • Certificate of Compliance
Submit a review Submit a question References (12)

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Western blot - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
  • Western blot - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
  • Western blot - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
  • Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14972] to CYP1B1 - C-terminal
  • Suitable for: WB, IHC-P
  • Reacts with: Rat, Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Recombinant Human CYP1B1 protein (ab114353)
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Overview

  • Product name

    Anti-CYP1B1 antibody [EPR14972] - C-terminal
    See all CYP1B1 primary antibodies
  • Description

    Rabbit monoclonal [EPR14972] to CYP1B1 - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human renal adenocarcinoma tissue. WB: Human breast cancer, fetal kidney and fetal brain lysate; Rat brain and spleen lysate.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR14972
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Neuroscience
    • Sensory System
    • Visual system
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Signal Transduction
    • Metabolism
    • Alcohol metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Alcohol metabolism

Associated products

  • Alternative Versions

    • Anti-CYP1B1 antibody [EPR14972] - BSA and Azide free (ab232645)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CYP1B1 protein (ab114353)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab185954 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Application notes
Is unsuitable for Flow Cyt.

Target

  • Function

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
    Participates in the metabolism of an as-yet-unknown biologically active molecule that is a participant in eye development.
  • Tissue specificity

    Expressed in many tissues.
  • Involvement in disease

    Defects in CYP1B1 are the cause of primary congenital glaucoma type 3A (GLC3A) [MIM:231300]. GLC3A is an autosomal recessive form of primary congenital glaucoma (PCG). PCG is characterized by marked increase of intraocular pressure at birth or early childhood, large ocular globes (buphthalmos) and corneal edema. It results from developmental defects of the trabecular meshwork and anterior chamber angle of the eye that prevent adequate drainage of aqueous humor.
    Defects in CYP1B1 are a cause of primary open angle glaucoma (POAG) [MIM:137760]. POAG is a complex and genetically heterogeneous ocular disorder characterized by a specific pattern of optic nerve and visual field defects. The angle of the anterior chamber of the eye is open, and usually the intraocular pressure is increased. The disease is asymptomatic until the late stages, by which time significant and irreversible optic nerve damage has already taken place. In some cases, POAG shows digenic inheritance involving mutations in CYP1B1 and MYOC genes.
    Defects in CYP1B1 are a cause of Peters anomaly (PAN) [MIM:604229]. Peters anomaly is a congenital defect of the anterior chamber of the eye.
  • Sequence similarities

    Belongs to the cytochrome P450 family.
  • Cellular localization

    Endoplasmic reticulum membrane. Microsome membrane.
  • Target information above from: UniProt accession Q16678 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1545 Human
    • Entrez Gene: 13078 Mouse
    • Entrez Gene: 25426 Rat
    • Omim: 601771 Human
    • SwissProt: Q16678 Human
    • SwissProt: Q64429 Mouse
    • SwissProt: Q64678 Rat
    • Unigene: 154654 Human
    • Unigene: 214016 Mouse
    • Unigene: 10125 Rat
    see all
  • Alternative names

    • Aryl hydrocarbon hydroxylase antibody
    • CP1B antibody
    • CP1B1_HUMAN antibody
    • Cyp1b1 antibody
    • CYPIB1 antibody
    • Cytochrome P450 1B1 antibody
    • Cytochrome P450 family 1 subfamily B polypeptide 1 antibody
    • Cytochrome P450 subfamily I (dioxin inducible) polypeptide 1 (glaucoma 3 primary infantile) antibody
    • Flavoprotein linked monooxygenase antibody
    • GLC3A antibody
    • Microsomal monooxygenase antibody
    • P4501B1 antibody
    • Xenobiotic monooxygenase antibody
    see all

Images

  • Western blot - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
    Western blot - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
    All lanes : Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954) at 1/20000 dilution

    Lane 1 : Human breast cancer lysate
    Lane 2 : Human fetal kidney lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 61 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)

    Immunohistochemical analysis of paraffin-embedded Human renal adenocarcinoma tissue labeling CYP1B1 with ab185954 at 1/500 dilution followed by pre-diluted HRP-conjugated secondary antibody and counter-stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
    Western blot - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
    All lanes : Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954) at 1/5000 dilution

    Lane 1 : Rat brain lysate
    Lane 2 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 61 kDa

  • Western blot - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
    Western blot - Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
    Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954) at 1/5000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 61 kDa

  • Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)
    Anti-CYP1B1 antibody [EPR14972] - C-terminal (ab185954)

Protocols

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

References (12)

Publishing research using ab185954? Please let us know so that we can cite the reference in this datasheet.

ab185954 has been referenced in 12 publications.

  • Yang L  et al. Immutol regulates CD4+Tregs, CD8+Tregs and pDCs via IDO signaling pathway to induce immune tolerance in rat heart allograft transplant. Transpl Immunol 68:101393 (2021). PubMed: 33838298
  • Bakewell S  et al. Inhibition of DNA Repair Pathways and Induction of ROS Are Potential Mechanisms of Action of the Small Molecule Inhibitor BOLD-100 in Breast Cancer. Cancers (Basel) 12:N/A (2020). PubMed: 32947941
  • Zhang Y  et al. Inhibition of CYP1B1 ameliorates cardiac hypertrophy induced by uremic toxin. Mol Med Rep 21:393-404 (2020). PubMed: 31746392
  • Shoieb SM & El-Kadi AOS Resveratrol attenuates angiotensin II-induced cellular hypertrophy through the inhibition of CYP1B1 and the cardiotoxic mid-chain HETE metabolites. Mol Cell Biochem 471:165-176 (2020). PubMed: 32533462
  • Borovok N  et al. Gene and Protein Expression in Subjects With a Nystagmus-Associated AHR Mutation. Front Genet 11:582796 (2020). PubMed: 33193710
View all Publications for this product

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