Overview

  • Product name

  • Description

    Rabbit polyclonal to CYP21A2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CYP21A2 aa 216-246 conjugated to keyhole limpet haemocyanin. The exact sequence is proprietary.
    Database link: P08686

  • Positive control

    • IHC-P: Human adrenal tissue. ICC/IF: HEK-293 cells. WB: MDA-MB435 cell lysate. Flow Cyt: MDA-MB231 cells.

Applications

Our Abpromise guarantee covers the use of ab230327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
ICC/IF 1/10 - 1/50.
WB 1/1000. Predicted molecular weight: 56 kDa.
Flow Cyt 1/10 - 1/50.

Target

  • Function

    Specifically catalyzes the 21-hydroxylation of steroids. Required for the adrenal synthesis of mineralocorticoids and glucocorticoids.
  • Involvement in disease

    Adrenal hyperplasia 3
  • Sequence similarities

    Belongs to the cytochrome P450 family.
  • Domain

    The leucine-rich hydrophobic amino acid N-terminal region probably helps to anchor the protein to the microsomal membrane.
  • Cellular localization

    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CYP21 antibody
    • 21 OHase antibody
    • 21-OHase antibody
    • CA21H antibody
    • CAH1 antibody
    • CP21A_HUMAN antibody
    • CPS1 antibody
    • CYP21A 2 antibody
    • CYP21A2 antibody
    • CYP21B antibody
    • Cytochrome P-450c21 antibody
    • Cytochrome P450 21 antibody
    • Cytochrome P450 C21B antibody
    • Cytochrome P450 XXI antibody
    • Cytochrome P450, family 21, subfamily A, polypeptide 2 antibody
    • Cytochrome P450, subfamily XXIA (steroid 21 hydroxylase, congenital adrenal hyperplasia), polypeptide 2 antibody
    • Cytochrome P450-C21 antibody
    • Cytochrome P450-C21B antibody
    • P450 C21 antibody
    • P450 C21B antibody
    • P450c21B antibody
    • Steroid 21 hydroxylase antibody
    • Steroid 21 monooxygenase antibody
    • Steroid 21-hydroxylase antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human adrenal tissue stained for CYP21A2 using ab230327 at 1/50 dilution in imunohistochemical analysis.

  • HEK-293 (human epithelial cell line from embryonic kidney) cells stained for CYP21A2 using ab230327 at 1/10 dilution, followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) in confocal immunofluorescence.

    Nucelar counterstain: DAPI (blue).

  • Anti-CYP21A2 antibody (ab230327) at 1/1000 dilution + MDA-MB-435S (Human ductal carcinoma cell line) cell lysate at 35 µg

    Predicted band size: 56 kDa

  • Flow cytometric analysis of MDA-MB231 (human breast adenocarcinoma cell line) cells labeling CYP21A2 with ab230327 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab230327 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab230327.
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