Key features and details
- Rabbit polyclonal to CYP24A1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CYP24A1 antibody
See all CYP24A1 primary antibodies
DescriptionRabbit polyclonal to CYP24A1
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Pig
Recombinant fragment, corresponding to a sequence within amino acids 241-498 of Human CYP24A1 (NP_000773).
- 293T, A431, H1299, HeLaS3, HepG2, Molt4 and Raji whole cell lysates.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
Our Abpromise guarantee covers the use of ab96691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 59 kDa.|
FunctionHas a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25-hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23-hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product.
Sequence similaritiesBelongs to the cytochrome P450 family.
- Information by UniProt
- 1 25 @dihydroxyvitamin D3 24 hydroxylase antibody
- 1 25 dihydroxyvitamin D(3) 24 hydroxylase antibody
- 1 antibody
All lanes : Anti-CYP24A1 antibody (ab96691) at 1/3000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : H1299 whole cell lysate
Lane 4 : HeLa S3 whole cell lysate
Lane 5 : HepG2 whole cell lysate
Lane 6 : MOLT4 whole cell lysate
Lane 7 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 59 kDa
7.5% SDS PAGE
ab96691 has been referenced in 2 publications.
- Porter RL et al. Epithelial to mesenchymal plasticity and differential response to therapies in pancreatic ductal adenocarcinoma. Proc Natl Acad Sci U S A N/A:N/A (2019). PubMed: 31843922
- Ge N et al. Associations between abnormal vitamin D metabolism pathway function and non-small cell lung cancer. Oncol Lett 14:7538-7544 (2017). PubMed: 29250167