Key features and details
- Rabbit polyclonal to CYP26A1
- Suitable for: IHC-P, WB
- Reacts with: Human, Pig
- Isotype: IgG
Product nameAnti-CYP26A1 antibody
See all CYP26A1 primary antibodies
DescriptionRabbit polyclonal to CYP26A1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human, Pig
Predicted to work with: Mouse
Recombinant fragment (His-T7-tag) corresponding to Human CYP26A1 aa 73-255. N-terminal tags. Expressed in E. coli.
RKYGFIYKTHLFGRPTVRVMGADNVRRILLGEHRLVSVHWPASVRTILGS GCLSNLHDSSHKQRKKVIMRAFSREALECYVPVITEEVGSSLEQWLSCGE RGLLVYPEVKRLMFRIAMRILLGCEPQLAGDGDSEQQLVEAFEEMTRNLF SLPIDVPFSGLYRGMKARNLIHARIEQNIRAKI
Database link: O43174
- WB: Recombinant human CYP26A1 protein; pig liver and heart tissue lysate; Jurkat whole cell lysate. IHC-P: Human breast cancer, lung and liver tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
Our Abpromise guarantee covers the use of ab232769 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 5 µg/ml. Predicted molecular weight: 56 kDa.|
FunctionPlays a key role in retinoic acid metabolism. Acts on retinoids, including all-trans-retinoic acid (RA) and its stereoisomer 9-cis-RA. Capable of both 4-hydroxylation and 18-hydroxylation. Responsible for generation of several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA and 18-OH-RA.
Tissue specificityHighest levels in adult liver, heart, pituitary gland, adrenal gland, placenta and regions of the brain.
Sequence similaritiesBelongs to the cytochrome P450 family.
Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
- Information by UniProt
- CP26 antibody
- CP26A_HUMAN antibody
- CYP26 antibody
All lanes : Anti-CYP26A1 antibody (ab232769) at 3 µg/ml
Lane 1 : Pig liver tissue lysate
Lane 2 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 3 : Pig heart tissue lysate
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 56 kDa
Formalin-fixed, paraffin-embedded human lung tissue stained for CYP26A1 using ab232769 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human liver tissue stained for CYP26A1 using ab232769 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human breast cancer tissue stained for CYP26A1 using ab232769 at 20 μg/ml in immunohistochemical analysis. DAB staining.
Anti-CYP26A1 antibody (ab232769) at 3 µg/ml + Recombinant human CYP26A1 protein
HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Predicted band size: 56 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab232769 has not yet been referenced specifically in any publications.