Recombinant Anti-CYP26A1 antibody [EPR11841] (ab172474)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR11841] to CYP26A1
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-CYP26A1 antibody [EPR11841]
See all CYP26A1 primary antibodies -
Description
Rabbit monoclonal [EPR11841] to CYP26A1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Human
Predicted to work with: RatDoes not react with: Mouse -
Immunogen
Recombinant fragment corresponding to Human CYP26A1 aa 250-450.
Database link: O43174 -
Positive control
- Human placenta, fetal liver and fetal kidney tissue lysates; 293T and HepG2 cell lysates; Human liver and pancreas tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR11841 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab172474 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 56 kDa.
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IHC-P |
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 56 kDa. |
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Plays a key role in retinoic acid metabolism. Acts on retinoids, including all-trans-retinoic acid (RA) and its stereoisomer 9-cis-RA. Capable of both 4-hydroxylation and 18-hydroxylation. Responsible for generation of several hydroxylated forms of RA, including 4-OH-RA, 4-oxo-RA and 18-OH-RA. -
Tissue specificity
Highest levels in adult liver, heart, pituitary gland, adrenal gland, placenta and regions of the brain. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1592 Human
- Entrez Gene: 154985 Rat
- Omim: 602239 Human
- SwissProt: O43174 Human
- Unigene: 150595 Human
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Alternative names
- CP26 antibody
- CP26A_HUMAN antibody
- CYP26 antibody
see all
Images
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Immunohistochemical analysis of paraffin embedded Human liver tissue labeling CYP26A1 with ab172474 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human pancreas tissue labeling CYP26A1 with ab172474 at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab172474 showing +ve staining in Human normal colon tissue. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab172474 showing +ve staining in Human normal uterus tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab172474 showing -ve staining in Human skeletal muscle tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab172474 showing -ve staining in Human heart muscle tissue.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-CYP26A1 antibody [EPR11841] (ab172474) at 1/1000 dilution
Lane 1 : Human placenta tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : Human fetal kidney tissue lysate
Lane 4 : 293T (Human embryonic kidney epithelial cell) cell lysate
Lane 5 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 56 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab172474 has been referenced in 3 publications.
- Yang N et al. Cardiac retinoic acid levels decline in heart failure. JCI Insight 6:N/A (2021). PubMed: 33724958
- Chen PH et al. CYP26A1 Is a Novel Biomarker for Betel Quid-Related Oral and Pharyngeal Cancers. Diagnostics (Basel) 10:N/A (2020). PubMed: 33233443
- Huang YJ et al. Protection against acetaminophen-induced acute liver failure by omentum adipose tissue derived stem cells through the mediation of Nrf2 and cytochrome P450 expression. J Biomed Sci 23:5 (2016). WB ; Mouse . PubMed: 26787241