Anti-CYP27A1 antibody (ab151987)
Key features and details
- Rabbit polyclonal to CYP27A1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CYP27A1 antibody
See all CYP27A1 primary antibodies -
Description
Rabbit polyclonal to CYP27A1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB MouseHuman
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Immunogen
Recombinant fragment corresponding to a region within amino acids 154-460 of Human CYP27A1.
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Positive control
- A549 and Mouse liver whole cell lysates; U87 xenograft tissue.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| IHC-P |
Human
|
| WB |
Mouse
Human
|
| All applications |
Monkey
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 60 kDa.
|
|
| IHC-P |
1/100 - 1/1000.
|
| Notes |
|---|
|
WB
1/500 - 1/3000. Predicted molecular weight: 60 kDa. |
|
IHC-P
1/100 - 1/1000. |
Target
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Function
Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity. -
Pathway
Hormone biosynthesis; cholecalciferol biosynthesis. -
Involvement in disease
Cerebrotendinous xanthomatosis -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Mitochondrion membrane. - Information by UniProt
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Database links
- Entrez Gene: 1593 Human
- Entrez Gene: 104086 Mouse
- Omim: 606530 Human
- SwissProt: Q02318 Human
- SwissProt: Q9DBG1 Mouse
- Unigene: 516700 Human
- Unigene: 85083 Mouse
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Alternative names
- 12-alpha-triol 27-hydroxylase antibody
- 5-beta-cholestane-3-alpha antibody
- 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase antibody
see all
Images
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Western blot - Anti-CYP27A1 antibody (ab151987)Anti-CYP27A1 antibody (ab151987) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 60 kDa
7.5% SDS PAGE -
Western blot - Anti-CYP27A1 antibody (ab151987)Anti-CYP27A1 antibody (ab151987) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 60 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody (ab151987)Immunohistochemical analysis of paraffin-embedded U87 (human) xenograft tissue labeling CYP27A1 with ab151987 at 1/500 dilution.
Protocols
References (0)
ab151987 has not yet been referenced specifically in any publications.
