Anti-CYP27A1 antibody (ab151987)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-CYP27A1 antibody
See all CYP27A1 primary antibodies -
Description
Rabbit polyclonal to CYP27A1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Monkey
-
Immunogen
Recombinant fragment corresponding to a region within amino acids 154-460 of Human CYP27A1.
-
Positive control
- A549 and Mouse liver whole cell lysates; U87 xenograft tissue.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78% PBS, 20% Glycerol, 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab151987 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 60 kDa. | |
| IHC-P | 1/100 - 1/1000. |
Target
-
Function
Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity. -
Pathway
Hormone biosynthesis; cholecalciferol biosynthesis. -
Involvement in disease
Cerebrotendinous xanthomatosis -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Mitochondrion membrane. - Information by UniProt
-
Database links
- Entrez Gene: 1593 Human
- Entrez Gene: 104086 Mouse
- Omim: 606530 Human
- SwissProt: Q02318 Human
- SwissProt: Q9DBG1 Mouse
- Unigene: 516700 Human
- Unigene: 85083 Mouse
-
Alternative names
- 12-alpha-triol 27-hydroxylase antibody
- 5-beta-cholestane-3-alpha antibody
- 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase antibody
see all
Images
-
Western blot - Anti-CYP27A1 antibody (ab151987)Anti-CYP27A1 antibody (ab151987) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 60 kDa
7.5% SDS PAGE -
Western blot - Anti-CYP27A1 antibody (ab151987)Anti-CYP27A1 antibody (ab151987) at 1/1000 dilution + Mouse liver whole cell lysate at 50 µg
Predicted band size: 60 kDa
7.5% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody (ab151987)Immunohistochemical analysis of paraffin-embedded U87 (human) xenograft tissue labeling CYP27A1 with ab151987 at 1/500 dilution.
References
ab151987 has not yet been referenced specifically in any publications.
