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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cholesterol Metabolism
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Anti-CYP27A1 antibody (ab230907)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody (ab230907)
  • Western blot - Anti-CYP27A1 antibody (ab230907)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody (ab230907)

Key features and details

  • Rabbit polyclonal to CYP27A1
  • Suitable for: IHC-P, WB
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human CYP27A1 protein (ab158258)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-CYP27A1 antibody
    See all CYP27A1 primary antibodies
  • Description

    Rabbit polyclonal to CYP27A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CYP27A1 aa 101-150. The exact sequence is proprietary.
    Database link: Q02318

  • Positive control

    • IHC-P: Human liver and kidney tissues. WB: HeLa cell extracts.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol

    (PBS without Mg2+ and Ca2+).
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cytochromes
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Cytochromes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
    • HeLa whole cell lysate (ab29545)
  • Recombinant Protein

    • Recombinant Human CYP27A1 protein (ab158258)
  • Related Products

    • Recombinant Human CYP27A1 protein (ab158258)

Applications

Our Abpromise guarantee covers the use of ab230907 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50.
WB 1/500 - 1/1000. Predicted molecular weight: 60 kDa.

Target

  • Function

    Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.
  • Pathway

    Hormone biosynthesis; cholecalciferol biosynthesis.
  • Involvement in disease

    Cerebrotendinous xanthomatosis
  • Sequence similarities

    Belongs to the cytochrome P450 family.
  • Cellular localization

    Mitochondrion membrane.
  • Target information above from: UniProt accession Q02318 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1593 Human
    • Omim: 606530 Human
    • SwissProt: Q02318 Human
    • Unigene: 516700 Human
    • Alternative names

      • 12-alpha-triol 27-hydroxylase antibody
      • 5-beta-cholestane-3-alpha antibody
      • 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase antibody
      • 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase antibody
      • 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase antibody
      • 7-alpha antibody
      • Cholestanetriol 26 monooxygenase antibody
      • CP27 antibody
      • CP27A_HUMAN antibody
      • CTX antibody
      • CYP antibody
      • CYP27 antibody
      • CYP27A1 antibody
      • Cytochrome P 450C27/25 antibody
      • Cytochrome P-450C27/25 antibody
      • Cytochrome P450 27 antibody
      • Cytochrome P450 27 mitochondrial antibody
      • Cytochrome P450 family 27 subfamily A member 1 antibody
      • Cytochrome P450 family 27 subfamily A polypeptide 1 antibody
      • Cytochrome P450 subfamily XXVIIA (steroid 27-hydroxylase cerebrotendinous xanthomatosis) polypeptide 1 antibody
      • mitochondrial antibody
      • Sterol 26 hydroxylase antibody
      • Sterol 26 hydroxylase mitochondrial antibody
      • Sterol 26-hydroxylase antibody
      • Sterol 27 hydroxylase antibody
      • Sterol 27-hydroxylase antibody
      • Vitamin D(3) 25 hydroxylase antibody
      • Vitamin D(3) 25-hydroxylase antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody (ab230907)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody (ab230907)

      Formalin-fixed, paraffin-embedded human kidney tissue stained for CYP27A1 using ab230907 at 1/50 dilution in immunohistochemical analysis.

    • Western blot - Anti-CYP27A1 antibody (ab230907)
      Western blot - Anti-CYP27A1 antibody (ab230907)
      All lanes : Anti-CYP27A1 antibody (ab230907) at 1/500 dilution

      Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
      Lane 2 : HeLa whole cell extract with immunizing peptide

      Predicted band size: 60 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody (ab230907)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody (ab230907)

      Formalin-fixed, paraffin-embedded human liver tissue stained for CYP27A1 using ab230907 at 1/50 dilution in immunohistochemical analysis.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (0)

    Publishing research using ab230907? Please let us know so that we can cite the reference in this datasheet.

    ab230907 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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