Anti-CYP27A1 antibody (ab230907)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-CYP27A1 antibody
See all CYP27A1 primary antibodies -
Description
Rabbit polyclonal to CYP27A1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human CYP27A1 aa 101-150. The exact sequence is proprietary.
Database link: Q02318 -
Positive control
- IHC-P: Human liver and kidney tissues. WB: HeLa cell extracts.
Properties
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol
(PBS without Mg2+ and Ca2+). -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab230907 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50. | |
| WB | 1/500 - 1/1000. Predicted molecular weight: 60 kDa. |
Target
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Function
Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity. -
Pathway
Hormone biosynthesis; cholecalciferol biosynthesis. -
Involvement in disease
Cerebrotendinous xanthomatosis -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Mitochondrion membrane. - Information by UniProt
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Database links
- Entrez Gene: 1593 Human
- Omim: 606530 Human
- SwissProt: Q02318 Human
- Unigene: 516700 Human
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Alternative names
- 12-alpha-triol 27-hydroxylase antibody
- 5-beta-cholestane-3-alpha antibody
- 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody (ab230907)Formalin-fixed, paraffin-embedded human kidney tissue stained for CYP27A1 using ab230907 at 1/50 dilution in immunohistochemical analysis.
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Western blot - Anti-CYP27A1 antibody (ab230907)All lanes : Anti-CYP27A1 antibody (ab230907) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : HeLa whole cell extract with immunizing peptide
Predicted band size: 60 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody (ab230907)Formalin-fixed, paraffin-embedded human liver tissue stained for CYP27A1 using ab230907 at 1/50 dilution in immunohistochemical analysis.
Datasheets and documents
References
ab230907 has not yet been referenced specifically in any publications.
