Name: Anti-CYP27A1 antibody [EPR7529]
Brand: RabMAb
SKU: ab126785
Rating: 5 (3 reviews)

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Primary - Rabbit Anti-CYP27A1 antibody [EPR7529] (ab126785)
WB, IHC-P, Flow Cyt, ICC/IF

Secondary - Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
IHC-P, WB, ELISA, IP

Protein - Recombinant Human CYP27A1 protein (ab158258)
WB, ELISA

View more associated products

Product name

Anti-CYP27A1 antibody [EPR7529]
See all CYP27A1 primary antibodies
Description

Rabbit monoclonal [EPR7529] to CYP27A1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Rat, Human
Immunogen

A synthetic peptide corresponding to residues in Human CYP27A1 (UniProt Q02318).
Positive control
- WB: HepG2, Caco 2, human fetal liver and mouse liver lysates. IHC-P: Human kidney and liver tissues. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Dissociation constant (K_D)

K_D = 6.00 x 10 ^-11 M
Learn more about K_D
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR7529
Isotype

IgG
Research areas

Alternative Versions
- Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free (ab214800)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human CYP27A1 protein (ab158258)

Our Abpromise guarantee covers the use of ab126785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB		1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P		1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt		1/120.
ICC/IF		1/50 - 1/100.

Application notes

Is unsuitable for IP.

Function

Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.
Pathway

Hormone biosynthesis; cholecalciferol biosynthesis.
Involvement in disease

Cerebrotendinous xanthomatosis
Sequence similarities

Belongs to the cytochrome P450 family.
Cellular localization

Mitochondrion membrane.
Target information above from: UniProt accession Q02318 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 1593 Human
- Entrez Gene: 104086 Mouse
- Entrez Gene: 301517 Rat
- Omim: 606530 Human
- SwissProt: Q02318 Human
- SwissProt: Q9DBG1 Mouse
- SwissProt: P17178 Rat
- Unigene: 516700 Human
- Unigene: 85083 Mouse
- Unigene: 94956 Rat
see all
Alternative names
- 12-alpha-triol 27-hydroxylase antibody
- 5-beta-cholestane-3-alpha antibody
- 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase antibody
- 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase antibody
- 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase antibody
- 7-alpha antibody
- Cholestanetriol 26 monooxygenase antibody
- CP27 antibody
- CP27A_HUMAN antibody
- CTX antibody
- CYP antibody
- CYP27 antibody
- CYP27A1 antibody
- Cytochrome P 450C27/25 antibody
- Cytochrome P-450C27/25 antibody
- Cytochrome P450 27 antibody
- Cytochrome P450 27 mitochondrial antibody
- Cytochrome P450 family 27 subfamily A member 1 antibody
- Cytochrome P450 family 27 subfamily A polypeptide 1 antibody
- Cytochrome P450 subfamily XXVIIA (steroid 27-hydroxylase cerebrotendinous xanthomatosis) polypeptide 1 antibody
- mitochondrial antibody
- Sterol 26 hydroxylase antibody
- Sterol 26 hydroxylase mitochondrial antibody
- Sterol 26-hydroxylase antibody
- Sterol 27 hydroxylase antibody
- Sterol 27-hydroxylase antibody
- Vitamin D(3) 25 hydroxylase antibody
- Vitamin D(3) 25-hydroxylase antibody
see all

Western blot - Anti-CYP27A1 antibody [EPR7529] (ab126785)

All lanes : Anti-CYP27A1 antibody [EPR7529] (ab126785) at 1/1000 dilution

Lane 1 : HepG2 lysate
Lane 2 : Caco 2 lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Mouse liver lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 60 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (ab126785)

ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
Immunocytochemistry/ Immunofluorescence - Anti-CYP27A1 antibody [EPR7529] (ab126785)

ab126785, at a 1/50 dilution, staining CYP27A1 in HepG2 cells by Immunofluorescence.
Flow Cytometry - Anti-CYP27A1 antibody [EPR7529] (ab126785)

Flow cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling CYP27A1 (red) with ab126785 at a 1/120 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (ab126785)

ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human liver tissue by Immunohistochemistry.
Other - Anti-CYP27A1 antibody [EPR7529] (ab126785)

Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D

Flow cytometry protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Datasheet

This product has been referenced in:

Donepudi AC et al. Deficiency of cholesterol 7a-hydroxylase in bile acid synthesis exacerbates alcohol-induced liver injury in mice. Hepatol Commun 2:99-112 (2018). Read more (PubMed: 29404516) »
Wang C et al. Morin protects glucocorticoid-induced osteoporosis through regulating the mitogen-activated protein kinase signaling pathway. J Nat Med 72:929-936 (2018). Read more (PubMed: 30043216) »

See all 10 Publications for this product

Publishing research using ab126785? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-3 of 3 Abreviews

Application

Western blot

Sample

Mouse Tissue lysate - whole (liver)

Gel Running Conditions

Non-reduced Denaturing (12%)

Loading amount

30 µg

Specification

liver

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

Abcam user community

Verified customer

Submitted Jul 04 2017

Application

Western blot

Loading amount

100000 cells

Gel Running Conditions

Reduced Denaturing

Sample

Rat Cell lysate - whole cell (rat vascular smooth muscle cells)

Specification

rat vascular smooth muscle cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Mar 23 2015

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Blocking step

Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: citrate buffer (pH 6.0)

Sample

Human Tissue sections (aortic atherosclerotic lesion)

Specification

aortic atherosclerotic lesion

Permeabilization

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Feb 07 2014

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Dissociation constant (KD)

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Compatible Secondaries

Isotype control

Recombinant Protein

Applications

Target

Function

Pathway

Involvement in disease

Sequence similarities

Cellular localization

Database links

Alternative names

Images

Protocols

Datasheets and documents

References

Customer reviews and Q&As

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Western blot abreview for Anti-CYP27A1 antibody [EPR7529]

Western blot abreview for Anti-CYP27A1 antibody [EPR7529]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CYP27A1 antibody [EPR7529]

Dissociation constant (K_D)