Recombinant Anti-CYP27A1 antibody [EPR7529] (ab126785)
![Western blot - Anti-CYP27A1 antibody [EPR7529] (ab126785)](https://www.abcam.com/ps/products/126/ab126785/Images/ab126785-140852-anti-cyp27a1-antibody-epr7529-western-blot.jpg "Western blot - Anti-CYP27A1 antibody [EPR7529] (ab126785)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7529] to CYP27A1
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-CYP27A1 antibody [EPR7529]
See all CYP27A1 primary antibodies -
Description
Rabbit monoclonal [EPR7529] to CYP27A1 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HepG2, Caco 2, human fetal liver and mouse liver lysates. IHC-P: Human kidney and liver tissues. ICC/IF: HepG2 cells. Flow Cyt (intra): HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 6.00 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7529 -
Isotype
IgG -
Research areas
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Cholesterol Metabolism
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126785 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt (Intra) |
1/120.
|
|
| WB | (3) |
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
|
| IHC-P | (2) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|
| ICC/IF |
1/50 - 1/100.
|
| Notes |
|---|
|
Flow Cyt (Intra)
1/120. |
|
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa. |
|
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
|
ICC/IF
1/50 - 1/100. |
Target
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Function
Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity. -
Pathway
Hormone biosynthesis; cholecalciferol biosynthesis. -
Involvement in disease
Cerebrotendinous xanthomatosis -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Mitochondrion membrane. - Information by UniProt
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Database links
- Entrez Gene: 1593 Human
- Entrez Gene: 104086 Mouse
- Entrez Gene: 301517 Rat
- Omim: 606530 Human
- SwissProt: Q02318 Human
- SwissProt: Q9DBG1 Mouse
- SwissProt: P17178 Rat
- Unigene: 516700 Human
see all -
Alternative names
- 12-alpha-triol 27-hydroxylase antibody
- 5-beta-cholestane-3-alpha antibody
- 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase antibody
see all
Images
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Western blot - Anti-CYP27A1 antibody [EPR7529] (ab126785)All lanes : Anti-CYP27A1 antibody [EPR7529] (ab126785) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : Caco 2 lysate
Lane 3 : Human fetal liver lysate
Lane 4 : Mouse liver lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 60 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (ab126785)](https://www.abcam.com/ps/products/126/ab126785/Images/ab126785-140851-anti-cyp27a1-antibody-epr7529-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (ab126785)ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![Immunocytochemistry/ Immunofluorescence - Anti-CYP27A1 antibody [EPR7529] (ab126785)](https://www.abcam.com/ps/products/126/ab126785/Images/ab126785-140849-anti-cyp27a1-antibody-epr7529-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-CYP27A1 antibody [EPR7529] (ab126785)ab126785, at a 1/50 dilution, staining CYP27A1 in HepG2 cells by Immunofluorescence. -
![Flow Cytometry (Intracellular) - Anti-CYP27A1 antibody [EPR7529] (ab126785)](https://www.abcam.com/ps/products/126/ab126785/Images/ab126785-280424-anti-cyp27a1-antibody-epr7529-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-CYP27A1 antibody [EPR7529] (ab126785)Intracellular Flow Cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling CYP27A1 (red) with ab126785 at a 1/120 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (ab126785)](https://www.abcam.com/ps/products/126/ab126785/Images/ab126785-140850-anti-cyp27a1-antibody-epr7529-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (ab126785)ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human liver tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-CYP27A1 antibody [EPR7529] (ab126785)](https://www.abcam.com/ps/products/126/ab126785/Images/ab126785-140848-anti-cyp27a1-antibody-epr7529.jpg)
OI-RD Scanning - Anti-CYP27A1 antibody [EPR7529] (ab126785) -
![Anti-CYP27A1 antibody [EPR7529] (ab126785)](https://www.abcam.com/ps/products/126/ab126785/Images/ab126785-7-benefits-of-recombinant-antibodies.png)
Anti-CYP27A1 antibody [EPR7529] (ab126785)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (20)
ab126785 has been referenced in 20 publications.
- Hutchinson SA et al. Liver x receptor alpha drives chemoresistance in response to side-chain hydroxycholesterols in triple negative breast cancer. Oncogene 40:2872-2883 (2021). PubMed: 33742124
- Sun C et al. Hepatic miR-378 modulates serum cholesterol levels by regulating hepatic bile acid synthesis. Theranostics 11:4363-4380 (2021). PubMed: 33754066
- Kiourtzidis M et al. Inhibition of Niemann-Pick C1-like protein 1 by ezetimibe reduces uptake of deuterium-labeled vitamin D in mice. J Steroid Biochem Mol Biol 197:105504 (2020). PubMed: 31682937
- Kimbung S et al. CYP27A1 expression is associated with risk of late lethal estrogen receptor-positive breast cancer in postmenopausal patients. Breast Cancer Res 22:123 (2020). PubMed: 33176848
- Huang F et al. Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism. Nat Commun 10:4971 (2019). PubMed: 31672964