Overview

  • Product name

    Anti-CYP27A1 antibody [EPR7529]
    See all CYP27A1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7529] to CYP27A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human CYP27A1 (UniProt Q02318).

  • Positive control

    • WB: HepG2, Caco 2, human fetal liver and mouse liver lysates. IHC-P: Human kidney and liver tissues. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab126785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/120.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.
    • Pathway

      Hormone biosynthesis; cholecalciferol biosynthesis.
    • Involvement in disease

      Cerebrotendinous xanthomatosis
    • Sequence similarities

      Belongs to the cytochrome P450 family.
    • Cellular localization

      Mitochondrion membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 12-alpha-triol 27-hydroxylase antibody
      • 5-beta-cholestane-3-alpha antibody
      • 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase antibody
      • 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase antibody
      • 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase antibody
      • 7-alpha antibody
      • Cholestanetriol 26 monooxygenase antibody
      • CP27 antibody
      • CP27A_HUMAN antibody
      • CTX antibody
      • CYP antibody
      • CYP27 antibody
      • CYP27A1 antibody
      • Cytochrome P 450C27/25 antibody
      • Cytochrome P-450C27/25 antibody
      • Cytochrome P450 27 antibody
      • Cytochrome P450 27 mitochondrial antibody
      • Cytochrome P450 family 27 subfamily A member 1 antibody
      • Cytochrome P450 family 27 subfamily A polypeptide 1 antibody
      • Cytochrome P450 subfamily XXVIIA (steroid 27-hydroxylase cerebrotendinous xanthomatosis) polypeptide 1 antibody
      • mitochondrial antibody
      • Sterol 26 hydroxylase antibody
      • Sterol 26 hydroxylase mitochondrial antibody
      • Sterol 26-hydroxylase antibody
      • Sterol 27 hydroxylase antibody
      • Sterol 27-hydroxylase antibody
      • Vitamin D(3) 25 hydroxylase antibody
      • Vitamin D(3) 25-hydroxylase antibody
      see all

    Images

    • All lanes : Anti-CYP27A1 antibody [EPR7529] (ab126785) at 1/1000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : Caco 2 lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : Mouse liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 60 kDa

    • ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human kidney tissue by Immunohistochemistry.
    • ab126785, at a 1/50 dilution, staining CYP27A1 in HepG2 cells by Immunofluorescence.
    • Flow cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling CYP27A1 (red) with ab126785 at a 1/120 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

    • ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human liver tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Donepudi AC  et al. Deficiency of cholesterol 7a-hydroxylase in bile acid synthesis exacerbates alcohol-induced liver injury in mice. Hepatol Commun 2:99-112 (2018). Read more (PubMed: 29404516) »
    • Wang C  et al. Morin protects glucocorticoid-induced osteoporosis through regulating the mitogen-activated protein kinase signaling pathway. J Nat Med 72:929-936 (2018). Read more (PubMed: 30043216) »
    See all 10 Publications for this product

    Customer reviews and Q&As

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    1-3 of 3 Abreviews

    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Gel Running Conditions
    Non-reduced Denaturing (12%)
    Loading amount
    30 µg
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C

    Abcam user community

    Verified customer

    Submitted Jul 04 2017

    Application
    Western blot
    Loading amount
    100000 cells
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Rat Cell lysate - whole cell (rat vascular smooth muscle cells)
    Specification
    rat vascular smooth muscle cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted Mar 23 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer (pH 6.0)
    Sample
    Human Tissue sections (aortic atherosclerotic lesion)
    Specification
    aortic atherosclerotic lesion
    Permeabilization
    No
    Fixative
    Formaldehyde

    Abcam user community

    Verified customer

    Submitted Feb 07 2014

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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