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RecombinantRabMAb

Recombinant Anti-CYP27A1 antibody [EPR7529] (ab126785)

  • Datasheet
  • SDS
Reviews (6)Q&A (4)References (16)

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Western blot - Anti-CYP27A1 antibody [EPR7529] (ab126785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (ab126785)
  • Immunocytochemistry/ Immunofluorescence - Anti-CYP27A1 antibody [EPR7529] (ab126785)
  • Flow Cytometry - Anti-CYP27A1 antibody [EPR7529] (ab126785)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (ab126785)
  • OI-RD Scanning - Anti-CYP27A1 antibody [EPR7529] (ab126785)
  • Anti-CYP27A1 antibody [EPR7529] (ab126785)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7529] to CYP27A1
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-CYP27A1 antibody [EPR7529]
    See all CYP27A1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7529] to CYP27A1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    A synthetic peptide corresponding to residues in Human CYP27A1 (UniProt Q02318).

  • Positive control

    • WB: HepG2, Caco 2, human fetal liver and mouse liver lysates. IHC-P: Human kidney and liver tissues. ICC/IF: HepG2 cells. Flow Cyt: HepG2 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Dissociation constant (KD)

    KD = 6.00 x 10 -11 M
    Learn more about KD
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7529
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cholesterol Metabolism
    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cytochromes
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Signal Transduction
    • Metabolism
    • Vitamins / Minerals
    • Signal Transduction
    • Metabolism
    • Lipid metabolism
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Cholesterol Metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases
    • Metabolism
    • Pathways and Processes
    • Cofactors, Vitamins / minerals
    • Vitamins / minerals
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Cytochromes
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free (ab214800)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human CYP27A1 protein (ab158258)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126785 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB (3)
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P (2)
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/120.
ICC/IF
1/50 - 1/100.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 60 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt
1/120.
ICC/IF
1/50 - 1/100.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.
    • Pathway

      Hormone biosynthesis; cholecalciferol biosynthesis.
    • Involvement in disease

      Cerebrotendinous xanthomatosis
    • Sequence similarities

      Belongs to the cytochrome P450 family.
    • Cellular localization

      Mitochondrion membrane.
    • Target information above from: UniProt accession Q02318 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1593 Human
      • Entrez Gene: 104086 Mouse
      • Entrez Gene: 301517 Rat
      • Omim: 606530 Human
      • SwissProt: Q02318 Human
      • SwissProt: Q9DBG1 Mouse
      • SwissProt: P17178 Rat
      • Unigene: 516700 Human
      • Unigene: 85083 Mouse
      • Unigene: 94956 Rat
      see all
    • Alternative names

      • 12-alpha-triol 27-hydroxylase antibody
      • 5-beta-cholestane-3-alpha antibody
      • 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase antibody
      • 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase antibody
      • 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase antibody
      • 7-alpha antibody
      • Cholestanetriol 26 monooxygenase antibody
      • CP27 antibody
      • CP27A_HUMAN antibody
      • CTX antibody
      • CYP antibody
      • CYP27 antibody
      • CYP27A1 antibody
      • Cytochrome P 450C27/25 antibody
      • Cytochrome P-450C27/25 antibody
      • Cytochrome P450 27 antibody
      • Cytochrome P450 27 mitochondrial antibody
      • Cytochrome P450 family 27 subfamily A member 1 antibody
      • Cytochrome P450 family 27 subfamily A polypeptide 1 antibody
      • Cytochrome P450 subfamily XXVIIA (steroid 27-hydroxylase cerebrotendinous xanthomatosis) polypeptide 1 antibody
      • mitochondrial antibody
      • Sterol 26 hydroxylase antibody
      • Sterol 26 hydroxylase mitochondrial antibody
      • Sterol 26-hydroxylase antibody
      • Sterol 27 hydroxylase antibody
      • Sterol 27-hydroxylase antibody
      • Vitamin D(3) 25 hydroxylase antibody
      • Vitamin D(3) 25-hydroxylase antibody
      see all

    Images

    • Western blot - Anti-CYP27A1 antibody [EPR7529] (ab126785)
      Western blot - Anti-CYP27A1 antibody [EPR7529] (ab126785)
      All lanes : Anti-CYP27A1 antibody [EPR7529] (ab126785) at 1/1000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : Caco 2 lysate
      Lane 3 : Human fetal liver lysate
      Lane 4 : Mouse liver lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 60 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (ab126785)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (ab126785)

      ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-CYP27A1 antibody [EPR7529] (ab126785)
      Immunocytochemistry/ Immunofluorescence - Anti-CYP27A1 antibody [EPR7529] (ab126785)
      ab126785, at a 1/50 dilution, staining CYP27A1 in HepG2 cells by Immunofluorescence.
    • Flow Cytometry - Anti-CYP27A1 antibody [EPR7529] (ab126785)
      Flow Cytometry - Anti-CYP27A1 antibody [EPR7529] (ab126785)

      Flow cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling CYP27A1 (red) with ab126785 at a 1/120 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (ab126785)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP27A1 antibody [EPR7529] (ab126785)

      ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human liver tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • OI-RD Scanning - Anti-CYP27A1 antibody [EPR7529] (ab126785)
      OI-RD Scanning - Anti-CYP27A1 antibody [EPR7529] (ab126785)
      Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD
    • Anti-CYP27A1 antibody [EPR7529] (ab126785)
      Anti-CYP27A1 antibody [EPR7529] (ab126785)

    Protocols

    • Flow cytometry protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (16)

    Publishing research using ab126785? Please let us know so that we can cite the reference in this datasheet.

    ab126785 has been referenced in 16 publications.

    • Kiourtzidis M  et al. Inhibition of Niemann-Pick C1-like protein 1 by ezetimibe reduces uptake of deuterium-labeled vitamin D in mice. J Steroid Biochem Mol Biol 197:105504 (2020). PubMed: 31682937
    • Huang F  et al. Theabrownin from Pu-erh tea attenuates hypercholesterolemia via modulation of gut microbiota and bile acid metabolism. Nat Commun 10:4971 (2019). PubMed: 31672964
    • Shi SZ  et al. Recruitment of monocytes and epigenetic silencing of intratumoral CYP7B1 primarily contribute to the accumulation of 27-hydroxycholesterol in breast cancer. Am J Cancer Res 9:2194-2208 (2019). PubMed: 31720082
    • Chen Y  et al. Association between the perturbation of bile acid homeostasis and valproic acid-induced hepatotoxicity. Biochem Pharmacol 170:113669 (2019). PubMed: 31628911
    • Yang F  et al. MicroRNA-559 plays an inhibitory role in the malignant progression of glioblastoma cells by directly targeting metadherin. Onco Targets Ther 12:4415-4426 (2019). PubMed: 31239710
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-6 of 6 Abreviews

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CYP27A1 antibody [EPR7529]

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Liver)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate buffer pH 6
    Permeabilization
    No
    Specification
    Liver
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 31 2020

    Western blot abreview for Anti-CYP27A1 antibody [EPR7529]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Liver)
    Gel Running Conditions
    Reduced Denaturing (10% acrylamide)
    Loading amount
    25 µg
    Specification
    Liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 31 2020

    IHC - Wholemount abreview for Anti-CYP27A1 antibody [EPR7529]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    IHC - Wholemount
    Sample
    Human Tissue (Liver)
    Specification
    Liver
    Read More

    Abcam user community

    Verified customer

    Submitted Jan 13 2020

    Western blot abreview for Anti-CYP27A1 antibody [EPR7529]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (liver)
    Gel Running Conditions
    Non-reduced Denaturing (12%)
    Loading amount
    30 µg
    Specification
    liver
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 04 2017

    Western blot abreview for Anti-CYP27A1 antibody [EPR7529]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    100000 cells
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Rat Cell lysate - whole cell (rat vascular smooth muscle cells)
    Specification
    rat vascular smooth muscle cells
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 23 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-CYP27A1 antibody [EPR7529]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 24°C
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer (pH 6.0)
    Sample
    Human Tissue sections (aortic atherosclerotic lesion)
    Specification
    aortic atherosclerotic lesion
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Feb 07 2014

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