Overview

  • Product name

    Anti-CYP27A1 antibody [EPR7529] - BSA and Azide free
    See all CYP27A1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7529] to CYP27A1 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human CYP27A1 (UniProt Q02318).

  • Positive control

    • HepG2, Caco 2, Human fetal liver and Mouse liver lysates; Human kidney and liver tissues; HepG2 cells.
  • General notes

    Ab214800 is the carrier-free version of ab126785. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab214800 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Applications

Our Abpromise guarantee covers the use of ab214800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 60 kDa.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Catalyzes the first step in the oxidation of the side chain of sterol intermediates; the 27-hydroxylation of 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol. Has also a vitamin D3-25-hydroxylase activity.
  • Pathway

    Hormone biosynthesis; cholecalciferol biosynthesis.
  • Involvement in disease

    Cerebrotendinous xanthomatosis
  • Sequence similarities

    Belongs to the cytochrome P450 family.
  • Cellular localization

    Mitochondrion membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 12-alpha-triol 27-hydroxylase antibody
    • 5-beta-cholestane-3-alpha antibody
    • 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 26-hydroxylase antibody
    • 5-beta-cholestane-3-alpha, 7-alpha, 12-alpha-triol 27-hydroxylase antibody
    • 5-beta-cholestane-3-alpha,7-alpha,12-alpha-triol 27-hydroxylase antibody
    • 7-alpha antibody
    • Cholestanetriol 26 monooxygenase antibody
    • CP27 antibody
    • CP27A_HUMAN antibody
    • CTX antibody
    • CYP antibody
    • CYP27 antibody
    • CYP27A1 antibody
    • Cytochrome P 450C27/25 antibody
    • Cytochrome P-450C27/25 antibody
    • Cytochrome P450 27 antibody
    • Cytochrome P450 27 mitochondrial antibody
    • Cytochrome P450 family 27 subfamily A member 1 antibody
    • Cytochrome P450 family 27 subfamily A polypeptide 1 antibody
    • Cytochrome P450 subfamily XXVIIA (steroid 27-hydroxylase cerebrotendinous xanthomatosis) polypeptide 1 antibody
    • mitochondrial antibody
    • Sterol 26 hydroxylase antibody
    • Sterol 26 hydroxylase mitochondrial antibody
    • Sterol 26-hydroxylase antibody
    • Sterol 27 hydroxylase antibody
    • Sterol 27-hydroxylase antibody
    • Vitamin D(3) 25 hydroxylase antibody
    • Vitamin D(3) 25-hydroxylase antibody
    see all

Images

  • ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human kidney tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126785).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Flow cytometry analysis of HepG2 (human hepatocellular carcinoma) cells labeling CYP27A1 (red) with ab126785 at a 1/120 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126785).

  • ab126785, at a 1/100 dilution, staining CYP27A1 in paraffin embedded Human liver tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126785).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • ab126785, at a 1/50 dilution, staining CYP27A1 in HepG2 cells by Immunofluorescence.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126785).

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab126785).

References

This product has been referenced in:

  • Soffientini U  et al. Intracellular cholesterol transporters and modulation of hepatic lipid metabolism: Implications for diabetic dyslipidaemia and steatosis. Biochim Biophys Acta 1842:1372-82 (2014). Read more (PubMed: 25014273) »
  • Aleksunes LM  et al. Repression of hepatobiliary transporters and differential regulation of classic and alternative bile acid pathways in mice during pregnancy. Toxicol Sci 130:257-68 (2012). WB ; Mouse . Read more (PubMed: 22903823) »
See all 2 Publications for this product

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