• Product name
  • Description
    Rabbit polyclonal to CYP27B1
  • Host species
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide corresponding to Human CYP27B1 aa 482-508 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: 1594

  • Positive control
    • WB: Mouse kidney tissue lysate. IHC-P: Human kidney tissue. Flow Cyt: 293 cells.


  • Form
  • Storage instructions
    Shipped at 4°C. Store at 4°C (up to 6 months). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    This antibody is prepared by Saturated Ammonium Sulfate precipitation followed by dialysis against PBS.
  • Clonality
  • Isotype


Our Abpromise guarantee covers the use of ab95047 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 57 kDa.
IHC-P 1/10 - 1/50.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.



  • Function
    Catalyzes the conversion of 25-hydroxyvitamin D3 (25(OH)D) to 1-alpha,25-dihydroxyvitamin D3 (1,25(OH)2D) plays an important role in normal bone growth, calcium metabolism, and tissue differentiation.
  • Tissue specificity
  • Pathway
    Hormone biosynthesis; cholecalciferol biosynthesis.
  • Involvement in disease
    Defects in CYP27B1 are the cause of rickets vitamin D-dependent type 1A (VDDR1A) [MIM:264700]; also known as pseudovitamin D deficiency rickets (PDDR). A disorder caused by a selective deficiency of the active form of vitamin D (1,25-dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Mitochondrion membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 1alpha(OH)ase antibody
    • 25-hydroxyvitamin D(3) 1-alpha-hydroxylase antibody
    • 25-hydroxyvitamin D-1 alpha hydroxylase antibody
    • 25-OHD-1 alpha-hydroxylase antibody
    • Calcidiol 1-monooxygenase antibody
    • CP27B_HUMAN antibody
    • CP2B antibody
    • CYP1 antibody
    • CYP1ALPHA antibody
    • CYP27B antibody
    • Cyp27b1 antibody
    • Cytochrome p450 27B1 antibody
    • Cytochrome p450 27B13 antibody
    • Cytochrome P450 family 27 subfamily B polypeptide 1 antibody
    • Cytochrome P450 subfamily XXVIIB (25-hydroxyvitamin D-1-alpha-hydroxylase) polypeptide 1 antibody
    • Cytochrome P450 subfamily XXVIIB polypeptide 1 antibody
    • Cytochrome P450C1 alpha antibody
    • Cytochrome P450VD1-alpha antibody
    • mitochondrial antibody
    • P450C1 alpha antibody
    • P450c1 antibody
    • P450C1-alpha antibody
    • P450VD1-alpha antibody
    • PDDR antibody
    • VD3 1A hydroxylase antibody
    • VDD1 antibody
    • VDDR antibody
    • VDDR I antibody
    • VDDRI antibody
    • VDR antibody
    see all


  • Anti-CYP27B1 antibody (ab95047) at 1/1000 dilution + T47D cell line lysates at 20 µg

    Goat anti-rabbit IgG H&L(HRP) at 1/10000 dilution

    Predicted band size: 57 kDa

  • Anti-CYP27B1 antibody (ab95047) at 1/1000 dilution + Mouse kidney tissue lysate at 35 µg

    Peroxidase-conjugated goat anti-rabbit IgG (H+L) at 1/5000 dilution

    Predicted band size: 57 kDa

    Incubation time was overnight at 4°C. Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling CYP27B1 with ab95047. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hour at 38°C; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hour at 37°C. A peroxidase-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

  • Flow Cytometry analysis of 293 cells labelling CYP27B1 with ab95047 (green) compared to a negative control (blue). The cells were fixed with paraformaldehyde and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 3% BSA to block non-specific protein-protein interactions followed by the primary antibody (1µg/1x106 cells) for 60 min at 37ºC. The secondary antibody, a FITC conjugated goat anti-rabbit IgG, was used at 1/200 dilution for 40 min at room temperature. Acquisition of >10,000 events was performed.


ab95047 has not yet been referenced specifically in any publications.

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