Anti-CYP2A6 antibody (ab3570)

Thank you for your response. While I cannot retroactively apply a discount to the order you have placed, the code will entitle you to xx off of one primary antibody for a future purchase. The code must be used on or before 10 October 2012. I would like to encourage you to take advantage of our Abreview program. It takes just a few minutes to leave a review and you can collect Abpoints which you may redeem for Abcam products or Amazon gift cards while at the same time sharing information about the product with your colleagues worldwide. To leave and Abreview, go to the webpage for the product, click on the Abreview Tab right below the shipping information and clickSubmit an Abreview. You can gain more Abpoints by submitting images as well. I hope that this information is helpful. Please let me know if you have any questions or there are other ways that Abcam may help you meet your research goals.

Thank you for contacting us. The recommended dilution of 1:150 is a recommendation which we make to our customers based on our experiments or those of supplier, collaborator or customer input. Although we cannot know exactly why certain products require higher dilutions in different protocols issues of affinity or reaction conditions will play a role. I would recommend using this product at the higher concentration recommended on the datasheet. I would also recommend running a positive control at the same time using the human liver Microsomes and strongly recommend using a loading control such as Beta Actin or GAPDH.They are used to normalize the levels of protein detected by confirming that protein loading is the same across the gel. The expression levels of the loading control should not vary between the different sample lanes. I have included links to these loading controls below. As you have not yet seen expression in your sample cell line I would also recommend a longer incubation, 4C overnight. We currently have a catalog of over 70,000 products. While we do make many of our products in house, we do receive others from outside suppliers. This product has been discontinued by our supplier; we do not know the exact cause for this discontinuation. We do offer a number of other CytochromeP450 2A6 antibodies which we do guarantee to work in western blot on cell lines. Our Rabbit polyclonal antibodies to Cytochrome P450 2A6, AB64890 or AB3570 may best suit your needs. As this product was discontinued shortly after your purchase I can offer you a discount off of anotherCytochrome P450 2A6 antibody should you decide to try one. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information. Beta Actin Loading Control:https://www.abcam.com/beta-Actin-antibody-mAbcam-8226-Loading-Control-ab8226.html GAPDH Loading Control:https://www.abcam.com/GAPDH-antibody-mAbcam-9484-Loading-Control-ab9484.html Further information about use of loading controls may be found at the following link:https://www.abcam.com/index.html?pageconfig=resource&rid=275 Two of our otherCytochrome P450 2A6 antibodies: AB3570:https://www.abcam.com/Cytochrome-P450-2A6-antibody-ab3570.html AB64890:https://www.abcam.com/Cytochrome-P450-2A6-antibody-ab64890.html