Anti-CYP2A6 antibody (ab3570)
Key features and details
- Rabbit polyclonal to CYP2A6
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-CYP2A6 antibody
See all CYP2A6 primary antibodies -
Description
Rabbit polyclonal to CYP2A6 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Rat
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Immunogen
Other Immunogen Type (His-tag) corresponding to Human CYP2A6. Purified, His-tagged, full length human P450 2A6 fusion protein.
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General notes
This product was previously labelled as Cytochrome P450 2A6
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
Purity
IgG fraction -
Primary antibody notes
The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab3570 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Rat
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| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/2000. Detects a band of approximately 49 kDa (predicted molecular weight: 57 kDa).
This antibody detects an ~49 kDa protein representing P450 2A6 from rat liver extract. |
| Notes |
|---|
|
WB
1/2000. Detects a band of approximately 49 kDa (predicted molecular weight: 57 kDa). This antibody detects an ~49 kDa protein representing P450 2A6 from rat liver extract. |
Target
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Function
Exhibits a high coumarin 7-hydroxylase activity. Can act in the hydroxylation of the anti-cancer drugs cyclophosphamide and ifosphamide. Competent in the metabolic activation of aflatoxin B1. Constitutes the major nicotine C-oxidase. Possesses low phenacetin O-deethylation activity. -
Tissue specificity
Liver. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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Alternative names
- Coumarin 7 hydroxylase antibody
- Coumarin 7-hydroxylase antibody
- CP2A6_HUMAN antibody
see all
Images
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Western blot - Anti-CYP2A6 antibody (ab3570)
ab3570 at a dilution of 1/2000 staining cytochrome P450 2A6 in rat liver lysate by Western blot.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab3570 has been referenced in 2 publications.
- Ranjit S et al. Extracellular Vesicles from Human Papilloma Virus-Infected Cervical Cancer Cells Enhance HIV-1 Replication in Differentiated U1 Cell Line. Viruses 12:N/A (2020). PubMed: 32098055
- Deng J et al. Circadian Regulation of Hepatic Cytochrome P450 2a5 by Peroxisome Proliferator-Activated Receptor ?. Drug Metab Dispos 46:1538-1545 (2018). PubMed: 30154104