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    cyp2c11-antibody-ab3571.pdf

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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cytochromes
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Anti-CYP2C11 antibody (ab3571)

  • Datasheet
  • SDS
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Immunocytochemistry/ Immunofluorescence - Anti-CYP2C11 antibody (ab3571)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C11 antibody (ab3571)
  • Immunocytochemistry/ Immunofluorescence - Anti-CYP2C11 antibody (ab3571)
  • Immunocytochemistry/ Immunofluorescence - Anti-CYP2C11 antibody (ab3571)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C11 antibody (ab3571)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C11 antibody (ab3571)

Key features and details

  • Rabbit polyclonal to CYP2C11
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-CYP2C11 antibody
  • Description

    Rabbit polyclonal to CYP2C11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide corresponding to Rat CYP2C11 aa 1-100.

    Synthetic peptide corresponding to Rat CYP2C11 aa 450-550.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Primary antibody notes

    The Cytochrome P450 (P450) family of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as NADPH and P450 reductase. There are over 200 cDNA’s which encode P450 protein. Epoxygenases are those P450 proteins which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450 proteins which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). EET’s, which exhibit vasodilation activity, are formed when an epoxide group is inserted between the unsaturated carbons of AA in positions 5,6; 8,9; 11,12; 14,15. EET’s are produced in cerebral cortical tissue, coronary arteries and vascular endothelium. EET’s are converted from AA by the 2C11 family of P450’s whose expression is induced by testosterone and is therefore not generally found in females.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cardiovascular
    • Lipids / Lipoproteins
    • Lipid Metabolism
    • Cytochromes
    • Signal Transduction
    • Metabolism
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipases
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Drug metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Cytochromes
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab3571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/20 - 1/200.
Notes
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/20 - 1/200.

Target

  • Function

    Metabolizes testosterone mainly in positions 2 alpha and 16 alpha.
  • Tissue specificity

    Liver; male-specific.
  • Sequence similarities

    Belongs to the cytochrome P450 family.
  • Cellular localization

    Endoplasmic reticulum membrane. Microsome membrane.
  • Target information above from: UniProt accession P08683 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 29277 Rat
    • SwissProt: P08683 Rat
    • Unigene: 10870 Rat
    • Alternative names

      • CP2CB_RAT antibody
      • Cyp2c antibody
      • Cyp2c11 antibody
      • CYP2CII antibody
      • CYPIIC11 antibody
      • Cytochrome P-450(M-1) antibody
      • Cytochrome P450 2C11 antibody
      • Cytochrome P450-UT-2 antibody
      • Cytochrome P450-UT-A antibody
      • Cytochrome P450H antibody
      • P450 UT A antibody
      • P450H antibody
      • UT2 antibody
      see all

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-CYP2C11 antibody (ab3571)
      Immunocytochemistry/ Immunofluorescence - Anti-CYP2C11 antibody (ab3571)

      ab3571 staining CYP2C11 (green) in HeLa (Human epithelial adenocarcinoma cell line) cells (right), compared to control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C11 antibody (ab3571)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C11 antibody (ab3571)

      ab3571 staining CYP2C11 in the cytoplasm of rat liver tissue (right) compared with a negative control in the absence of primary antibody (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were then blocked in 3% H2O2-methanol for 15 min at room temperature. Sections were incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunocytochemistry/ Immunofluorescence - Anti-CYP2C11 antibody (ab3571)
      Immunocytochemistry/ Immunofluorescence - Anti-CYP2C11 antibody (ab3571)

      ab3571 staining CYP2C11 (green) in PC-12 (Rat adrenal gland pheochromocytoma cell line) cells (right), compared to control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

    • Immunocytochemistry/ Immunofluorescence - Anti-CYP2C11 antibody (ab3571)
      Immunocytochemistry/ Immunofluorescence - Anti-CYP2C11 antibody (ab3571)

      ab3571 staining CYP2C11 (green) in H-4-II-E (Rat hepatoma cell line) cells (right), compared to control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C11 antibody (ab3571)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C11 antibody (ab3571)

      ab3571 staining CYP2C11 in the cytoplasm of rat kidney tissue (right) compared with a negative control in the absence of primary antibody (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were then blocked in 3% H2O2-methanol for 15 min at room temperature. Sections were incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C11 antibody (ab3571)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C11 antibody (ab3571)
      IHC image of ab3571 staining in human renal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3571, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    Protocols

    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (12)

    Publishing research using ab3571? Please let us know so that we can cite the reference in this datasheet.

    ab3571 has been referenced in 12 publications.

    • Li Z  et al. Disease Status-Dependent Drug-Herb Interactions: NASH Lowered the Risk of Hepatotoxicity in Rats Coadministered With Simvastatin and Gardenia jasminoides J. Ellis. Front Pharmacol 12:622040 (2021). PubMed: 33967756
    • Doan TNK  et al. Differential Effects of 1a,25-Dihydroxyvitamin D3 on the Expressions and Functions of Hepatic CYP and UGT Enzymes and Its Pharmacokinetic Consequences In Vivo. Pharmaceutics 12:N/A (2020). PubMed: 33238436
    • Li H  et al. Vascular Protection of TPE-CA on Hyperhomocysteinemia-induced Vascular Endothelial Dysfunction through AA Metabolism Modulated CYPs Pathway. Int J Biol Sci 15:2037-2050 (2019). PubMed: 31592228
    • Xiao M  et al. Impact of the Chinese herbal medicines on dual antiplatelet therapy with clopidogrel and aspirin: Pharmacokinetics and pharmacodynamics outcomes and related mechanisms in rats. J Ethnopharmacol 235:100-110 (2019). PubMed: 30710735
    • Kot M  et al. The Effect of Chronic Treatment with Lurasidone on Rat Liver Cytochrome P450 Expression and Activity in the Chronic Mild Stress Model of Depression. Drug Metab Dispos 45:1336-1344 (2017). PubMed: 28935656
    View all Publications for this product

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