Overview

  • Product name

    Anti-CYP2C11 antibody
  • Description

    Rabbit polyclonal to CYP2C11
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, ELISA, ICC, IHC-Fr, WBmore details
  • Species reactivity

    Reacts with: Rat, Cat, Human
  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide corresponding to Rat CYP2C11 aa 49-60.
    Sequence: DIGQSIKKFSKV

    Synthetic peptide corresponding to Rat CYP2C11 aa 491-500.
    Sequence:

    QRADSLSSHL

  • General notes

     This product was previously labelled as Cytochrome P450 2C11

     

Properties

Applications

Our Abpromise guarantee covers the use of ab3571 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/20 - 1/200.
ELISA Use at an assay dependent concentration.
ICC Use at an assay dependent concentration.
IHC-Fr 1/100.

Immunohistochemical staining of P450 2C11 in rat brain results in perivascular staining.

WB 1/1500.

By Western blot, this antibody detects an ~50 kDa protein representing P450 2C11 from rat liver extract.

Target

  • Function

    Metabolizes testosterone mainly in positions 2 alpha and 16 alpha.
  • Tissue specificity

    Liver; male-specific.
  • Sequence similarities

    Belongs to the cytochrome P450 family.
  • Cellular localization

    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CP2CB_RAT antibody
    • Cyp2c antibody
    • Cyp2c11 antibody
    • CYP2CII antibody
    • CYPIIC11 antibody
    • Cytochrome P-450(M-1) antibody
    • Cytochrome P450 2C11 antibody
    • Cytochrome P450-UT-2 antibody
    • Cytochrome P450-UT-A antibody
    • Cytochrome P450H antibody
    • P450 UT A antibody
    • P450H antibody
    • UT2 antibody
    see all

Images

  • ab3571 staining CYP2C11 (green) in HeLa cells (right), compared to control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • ab3571 staining CYP2C11 (green) in PC12 cells (right), compared to control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • ab3571 staining CYP2C11 (green) in H-4-II-E cells (right), compared to control (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were incubated with primary antibody (1:100 in 3% BSA-PBS) overnight at 4 ºC. A DyLight-conjugated anti-rabbit was used as the secondary antibody. Red (phalloidin) - F-actin, Blue - nuclei. Images were taken at a magnification of 60x.

  • ab3571 staining CYP2C11 in the cytoplasm of Rat liver tissue (right) compared with a negative control in the absence of primary antibody (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were then blocked in 3% H2O2-methanol for 15 min at room temperature. Sections were incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • ab3571 staining CYP2C11 in the cytoplasm of Rat kidney tissue (right) compared with a negative control in the absence of primary antibody (left) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). To expose target proteins, antigen retrieval method was performed using 10mM sodium citrate (pH 6.0) microwaved for 8-15 min. Tissues were then blocked in 3% H2O2-methanol for 15 min at room temperature. Sections were incubated with primary antibody (1:200 in 3% BSA-PBS) overnight at 4°C. A HRP-conjugated anti-rabbit was used as the secondary antibody, followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.

  • IHC image of ab3571 staining in human renal carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3571, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References

This product has been referenced in:

  • Xiao M  et al. Impact of the Chinese herbal medicines on dual antiplatelet therapy with clopidogrel and aspirin: Pharmacokinetics and pharmacodynamics outcomes and related mechanisms in rats. J Ethnopharmacol 235:100-110 (2019). Read more (PubMed: 30710735) »
  • Xu M  et al. Cardiotonic Pill Reduces Myocardial Ischemia-Reperfusion Injury via Increasing EET Concentrations in Rats. Drug Metab Dispos 44:878-87 (2016). Read more (PubMed: 27149899) »
See all 7 Publications for this product

Customer reviews and Q&As

Filter by Application

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1-2 of 2 Abreviews

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Lung)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric buffer
Permeabilization
Yes - Triton X-100
Specification
Lung
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 08 2019

Application
Western blot
Sample
Rat Cell lysate - whole cell (Liver)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
30 µg
Specification
Liver
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

Submitted Jul 02 2019

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