Recombinant
RabMAb

Recombinant Anti-CYP2C19 antibody [EPR6576] (ab137015)

Overview

  • Product name

    Anti-CYP2C19 antibody [EPR6576]
    See all CYP2C19 primary antibodies
  • Description

    Rabbit monoclonal [EPR6576] to CYP2C19
  • Host species

    Rabbit
  • Specificity

    This antibody is very likely to be cross-reactive with many Cytochrome P450 family members. According to the blast result, in human species, the immunogen has 100% homology with CYP2C9, CYP2A6 and CYP2A7, 85.7% with CYP2C18, 78.6% with CYP2A13 and CYP2C8. CYP2C19 doesn`t exist in mouse and rat species. The immunogen shares 100% homology with mouse CYP2A4, CYP2A5, CYP2A12, CYP2C29, CYP2C70 and rat CYP2A3, CYP2C6, CYP2C55, 92.9% with mouse CYP2C37, CYP2C50, CYP2C55 and rat CYP2C11, CYP2C13.
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human CYP2C19 aa 1-100. The exact sequence is proprietary.

  • Positive control

    • WB: Mouse liver, rat liver, Human fetal liver lysates IHC-P: Human liver, mouse liver, and rat liver tissue
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Dissociation constant (KD)

    KD = 1.24 x 10 -10 M
    Learn more about KD
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR6576
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab137015 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/1000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

For unpurified use at 1/250 - 1/500

WB 1/1000 - 1/10000. Predicted molecular weight: 56 kDa.

Target

  • Relevance

    Responsible for the metabolism of a number of therapeutic agents such as the anticonvulsant drug S-mephenytoin, omeprazole, proguanil, certain barbiturates, diazepam, propranolol, citalopram and imipramine.
  • Cellular localization

    Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
  • Database links

  • Alternative names

    • (R)-limonene 6-monooxygenase antibody
    • (S)-limonene 6-monooxygenase antibody
    • (S)-limonene 7-monooxygenase antibody
    • CYP2C19 antibody
    • CYPIIC17 antibody
    • CYPIIC19 antibody
    • Cytochrome P450 2C19 antibody
    • Cytochrome P450-11A antibody
    • Cytochrome P450-254C antibody
    • Mephenytoin 4-hydroxylase antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat liver tissue sections labeling CYP2C19 with Purified ab137015 at 1:1000 dilution (0.49 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylinwas used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse liver tissue sections labeling CYP2C19 with Purified ab137015 at 1:1000 dilution (0.49 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylinwas used as a counterstain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling CYP2C19 with Purified ab137015 at 1:1000 dilution (0.49 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0) ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylinwas used as a counterstain.

  • All lanes : Anti-CYP2C19 antibody [EPR6576] (ab137015) at 0.5 µg/ml

    Lane 1 : Mouse liver lysates
    Lane 2 : Rat liver lysates

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 56 kDa



    Blocking and diluting buffer: 5% NFDM/TBST.

  • All lanes : Anti-CYP2C19 antibody [EPR6576] (ab137015) at 0.5 µg/ml (purified)

    Lane 1 : Human fetal liver lysates
    Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates, negative control

    Lysates/proteins at 15 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 56 kDa



    Blocking and diluting buffer: 5% NFDM/TBST. 
    The doublet should be due to the cross-reaction with family members. 


  • Immunohistochemical analysis of paraffin-embedded Human liver tissue labelling CYP2C19 with unpurified ab137015 at 1/250 dilution.

  • All lanes : Anti-CYP2C19 antibody [EPR6576] (ab137015) at 1/1000 dilution (Unpurified)

    Lane 1 : Mouse liver lysate
    Lane 2 : Rat liver lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 56 kDa

  • Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

References

This product has been referenced in:

  • Tanino T  et al. Hepatic Cytochrome P450 Activity and Nitric Oxide Production During Multiple Ovalbumin Challenges. Eur J Drug Metab Pharmacokinet N/A:N/A (2018). Read more (PubMed: 30411300) »
  • Shi C  et al. Peroxisome Proliferator-Activated Receptor a Activation Suppresses Cytochrome P450 Induction Potential in Mice Treated with Gemfibrozil. Basic Clin Pharmacol Toxicol 121:169-174 (2017). Read more (PubMed: 28374976) »
See all 5 Publications for this product

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