Anti-CYP2C8 antibody (ab88904)

Submit a review Q&A (2)

Overview

  • Product name

    Anti-CYP2C8 antibody
    See all CYP2C8 primary antibodies

  • Description

    Mouse polyclonal to CYP2C8

  • Host species

    Mouse

  • Tested applications

    Suitable for: WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Full length Human CYP2C8 (AAH20596.1, amino acids 1-490)

  • Positive control

    • Human liver tissue lysate; CYP2C8 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab88904 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 56 kDa.

Target

  • Function

    Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. In the epoxidation of arachidonic acid it generates only 14,15- and 11,12-cis-epoxyeicosatrienoic acids. It is the principal enzyme responsible for the metabolism the anti-cancer drug paclitaxel (taxol).

  • Sequence similarities

    Belongs to the cytochrome P450 family.

  • Cellular localization

    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt

  • Database links

    • Alternative names

      • CP2C8_HUMAN antibody
      • CPC8 antibody
      • CYP2C8 antibody
      • CYPIIC8 antibody
      • Cytochrome P450 2C8 antibody
      • Cytochrome P450 family 2 subfamily C polypeptide 8 antibody
      • Cytochrome P450 form 1 antibody
      • Cytochrome P450 IIC2 antibody
      • Cytochrome P450 MP-12 antibody
      • Cytochrome P450 MP-20 antibody
      • Cytochrome P450 subfamily IIC (mephenytoin 4 hydroxylase) polypeptide 8 antibody
      • Flavoprotein linked monooxygenase antibody
      • Microsomal monooxygenase antibody
      • MP 12/MP 20 antibody
      • P450 form 1 antibody
      • P450 IIC2 antibody
      • P450 MP 12/MP 20 antibody
      • S mephenytoin 4 hydroxylase antibody
      • S-mephenytoin 4-hydroxylase antibody
      • Xenobiotic monooxygenase antibody
      see all

    Images

    • Western blot - Anti-CYP2C8 antibody (ab88904)
      Western blot - Anti-CYP2C8 antibody (ab88904)
      Anti-CYP2C8 antibody (ab88904) at 1/500 dilution + human liver tissue lysate at 50 µg

      Predicted band size: 56 kDa
      Observed band size: 50 kDa
      why is the actual band size different from the predicted?

    • Western blot - Anti-CYP2C8 antibody (ab88904)
      Western blot - Anti-CYP2C8 antibody (ab88904)
      All lanes : Anti-CYP2C8 antibody (ab88904) at 1/500 dilution

      Lane 1 : CYP2C8 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate

      Lysates/proteins at 25 µg per lane.

      Predicted band size: 56 kDa
      Observed band size: 48 kDa why is the actual band size different from the predicted?

    References

    ab88904 has not yet been referenced specifically in any publications.

    Publishing research using ab88904? Please let us know so that we can cite the reference in this datasheet.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-2 of 2 Abreviews or Q&A

    Question

    No bands in Western blot.

    Read More
    Answer

    Thank you very much for your call today and for letting us know about the trouble with this antibody.

    As we discussed, I'm sending a free of charge vial of ab126844 on the order ****. Please keep me updated about the results with this replacement antibody once you've had a chance to try it.

    If there is anything else that we can do for you, please let me know and I'll be happy to help. Have a nice day.

    Read More

    Question

    In reference to our telephonic conversation about Anti-CYP2C8 antibody (ab88904) I would like to inquire about the antibody reactivity with different truncated products which I plan to make with original (Wild Type) CYP2C8 which is protein sequence of 490 amino acid in length. These truncated products are the variants I plan to detect cytochrome P450 2C8 isoform b [Homo sapiens] 420 aa protein and also cytochrome P450 2C8 isoform c [Homo sapiens] 388 aa protein using this antibody.
    I was wanting to know if this antibody would work good for detection of this variants, since these variants are N- terminal truncated products, so if the antibody is generated to the N-terminal end of the protein it think it can give me problems. For better understanding of the sequence these variants share, I am attaching the sequence similarity match as attachment, Please have take a look.
    Isoform 1 is WT;
    Isoform 2 is cytochrome P450 2C8 isoform b;
    Isoform 3 is cytochrome P450 2C8 isoform c
    Hoping to hear from you soon. Thanks.

    Read More
    Answer

    Thank you for your enquiry.

    Here is the peptide immunogeninformation for ab126844:

    Synthetic peptide: KNVALTRSYIREK (Human) by a Cysteine residue linker from the internal region of Human CYP2C8 (NP_000761.3; NP_001185782.1; NP_001185783.1).

    I hope this is helpful. Please contact me again if you have any further questions.

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com