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    cyp2c9-antibody-ab4236.pdf

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Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cytochromes
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Anti-CYP2C9 antibody (ab4236)

  • Datasheet
Reviews (2)Q&A (5)References (6)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C9 antibody (ab4236)

    Key features and details

    • Rabbit polyclonal to CYP2C9
    • Suitable for: IHC-P
    • Reacts with: Human
    • Isotype: IgG

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    Overview

    • Product name

      Anti-CYP2C9 antibody
      See all CYP2C9 primary antibodies
    • Description

      Rabbit polyclonal to CYP2C9
    • Host species

      Rabbit
    • Tested Applications & Species

      Application Species
      IHC-P
      Human
      See all applications and species data
    • Immunogen

      Recombinant full length protein corresponding to Human CYP2C9.

    • General notes


      The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.

       This product was previously labelled as Cytochrome P450 2C9

       

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      pH: 8.00
      Constituent: 0.0536% PBS
    • Concentration information loading...
    • Purity

      Ammonium Sulphate Precipitation
    • Primary antibody notes

      The Cytochrome P450 (P450) superfamily of enzymes is one of three enzyme systems which metabolize the fatty acid arachadonic acid (AA) to regulators of vascular tone. P450 enzymes are monooxygenase enzymes which require several co-factors such as nicotinamide adenine dinucleotide phosphate (NADPH) and P450 reductase. There are over 200 known genes which encode P450s. Epoxygenases are those P450s which metabolize AA to epoxyeicosatrienoic acid (EETs) and omega-hydroxylases are those P450s which produce 19- and 20-hydroxyeicosatetraenoic acids (19- and 20-HETE). As well as fatty acid metabolism, P450s also metabolize many drugs and toxins. Cytochrome P450 3A4 is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipid Metabolism
      • Cytochromes
      • Signal Transduction
      • Metabolism
      • Lipid metabolism
      • Signal Transduction
      • Metabolism
      • Drug metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipases
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Drug metabolism
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Cytochromes
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab4236 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    IHC-P
    Human
    Application Abreviews Notes
    IHC-P
    Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
    Notes
    IHC-P
    Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    Target

    • Function

      Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This enzyme contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S-warfarin, diclofenac, phenytoin, tolbutamide and losartan.
    • Sequence similarities

      Belongs to the cytochrome P450 family.
    • Cellular localization

      Endoplasmic reticulum membrane. Microsome membrane.
    • Target information above from: UniProt accession P11712 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1559 Human
      • Omim: 601130 Human
      • SwissProt: P11712 Human
      • Unigene: 282624 Human
      • Alternative names

        • (R)-limonene 6-monooxygenase antibody
        • (S)-limonene 6-monooxygenase antibody
        • (S)-limonene 7-monooxygenase antibody
        • CP2C9_HUMAN antibody
        • CPC9 antibody
        • CYP2C antibody
        • CYP2C10 antibody
        • CYP2C9 antibody
        • CYPIIC9 antibody
        • cytochrome P-450 S-mephenytoin 4-hydroxylase antibody
        • Cytochrome P-450MP antibody
        • Cytochrome P450 2C9 antibody
        • Cytochrome P450 MP-4 antibody
        • Cytochrome P450 MP-8 antibody
        • Cytochrome P450 PB-1 antibody
        • Cytochrome P450, family 2, subfamily C, polypeptide 9 antibody
        • Cytochrome p4502C9 antibody
        • flavoprotein-linked monooxygenase antibody
        • MGC149605 antibody
        • MGC88320 antibody
        • microsomal monooxygenase antibody
        • OTTHUMP00000020135 antibody
        • P450 MP antibody
        • P450 PB 1 antibody
        • P450 PB1 antibody
        • P450IIC9 antibody
        • P450MP antibody
        • S mephenytoin 4 hydroxylase antibody
        • S-mephenytoin 4-hydroxylase antibody
        • xenobiotic monooxygenase antibody
        see all

      Images

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C9 antibody (ab4236)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP2C9 antibody (ab4236)
        IHC image of ab4236 staining in human liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab4236, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

        For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

      Protocols

      • Immunohistochemistry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (6)

      Publishing research using ab4236? Please let us know so that we can cite the reference in this datasheet.

      ab4236 has been referenced in 6 publications.

      • Sekretarska J  et al. Influence of Selected Carbon Nanostructures on the CYP2C9 Enzyme of the P450 Cytochrome. Materials (Basel) 12:N/A (2019). PubMed: 31835701
      • Guo R  et al. Chemical Cocktails Enable Hepatic Reprogramming of Mouse Fibroblasts with a Single Transcription Factor. Stem Cell Reports 9:499-512 (2017). PubMed: 28757167
      • Cichocki JA  et al. Impact of Nonalcoholic Fatty Liver Disease on Toxicokinetics of Tetrachloroethylene in Mice. J Pharmacol Exp Ther 361:17-28 (2017). PubMed: 28148637
      • Skrypnyk N  et al. PPARa activation can help prevent and treat non-small cell lung cancer. Cancer Res 74:621-31 (2014). PubMed: 24302581
      • Irusta G  et al. Dynamic expression of epoxyeicosatrienoic acid synthesizing and metabolizing enzymes in the primate corpus luteum. Mol Hum Reprod 13:541-8 (2007). PubMed: 17567599
      • Vriens J  et al. Modulation of the Ca2 permeable cation channel TRPV4 by cytochrome P450 epoxygenases in vascular endothelium. Circ Res 97:908-15 (2005). PubMed: 16179585

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-7 of 7 Abreviews or Q&A

      Question

      I am sending image of WB with the 2 antibodies I used:
      Anti-Cytochrome P450 4A11/22 antibody (ab110746)
      Anti-Cytochrome P450 2C9 antibody (ab4236)
      My WB protocol:
      Blocking - 5% milk/1 hour/ room temp
      Wash - t-tbs/5 min/ room temp
      Primary antibody - overnight/ 1:1000 in 5% milk/ 4C
      3x wash - t-tbs/5 min/ room temp
      Secondary antibody - 1 hour/ 1:10000 in 5% milk/ room temp
      3x wash - t-tbs/5 min/ room temp

      Read More

      Abcam community

      Verified customer

      Asked on Mar 06 2012

      Answer

      Thank you for your reply and for sending your data. I agree that the results you have obtained are difficult to interpret. For that reason, I would be happy to offer you free of charge replacements, or a credit or refund. Please let me know how you would like to proceed and I will be happy to assist you further.

      Read More

      Abcam Scientific Support

      Answered on Mar 06 2012

      Question

      Do you sell blocking peptides for ab4236 and ab110746?

      Read More

      Abcam community

      Verified customer

      Asked on Mar 05 2012

      Answer

      Thank you for contacting us.
      Because ab4236 is a rabbit polyclonal antibody that was raised against a full length protein, you could use a full length recombinant Cytochrome P450 2C9 protein such as ab56998 for blocking. It is very difficult and expensive to achieve full blocking using a full length protein, however you may be able to observe a reduction in the specific signal by pre-incubation with this product. After checking with the lab, a blocking peptide for ab110746 is not currently available.
      I am sorry I could not be more helpful, please let me know if you need any additional information or assistance.

      Read More

      Abcam Scientific Support

      Answered on Mar 05 2012

      Question

      I would like to test ab4236 for use on rat samples.

      Read More

      Abcam community

      Verified customer

      Asked on Jan 26 2012

      Answer

      DISCOUNT CODE: ***
      Expiration date: May 26th, 2012

      I am very pleased to hear you would like to accept our offer and test ab4236 in rat. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for rat and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


      Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

      Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

      The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

      Read More

      Abcam Scientific Support

      Answered on Jan 26 2012

      Question

      To whom this may concern,   I would like to request for a Certificate of Compliance for each of the following antibodies.   Product No. ab4236 with Lot No. GR21040 Product No. ab22717 with Lot No. GR34397 Product No. ab62204 with Lot No. GR6576   Please send these certificates to this email address. Let me know if you have any questions or concerns regarding this email.  

      Read More

      Abcam community

      Verified customer

      Asked on Dec 06 2011

      Answer

      Thank you for contacting Abcam. Please find attached the requested Certificate of Compliance

      Read More

      Abcam Scientific Support

      Answered on Dec 07 2011

      Western blot abreview for Anti-CYP2C9 antibody

      Inconclusive
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Sheep Tissue lysate - whole (Liver)
      Gel Running Conditions
      Reduced Denaturing (4-12% Bis-Tris Gel)
      Loading amount
      80 µg
      Specification
      Liver
      Blocking step
      Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More

      Mr. Joseph Statwick

      Verified customer

      Submitted Mar 10 2011

      Western blot abreview for Anti-CYP2C9 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Mouse Tissue lysate - whole (liver)
      Gel Running Conditions
      Reduced Denaturing (4-20% gel)
      Loading amount
      30 µg
      Specification
      liver
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 13 2010

      Question

      What do you recommend as positive and negative controls for these antibodies?

      Read More

      Abcam community

      Verified customer

      Asked on Nov 27 2006

      Answer

      Thank you for contacting us. For ab23677, LTA4 hydrolase, I suggest either a neutrophil or monocyte lysate. We carry THP1 lysate, ab7913, which is a human monocytic leukemia. For a negative control, LTA4 was detected in epidermal keratinocytes and endothelial cells but not whole skin in the following paper: Leukotriene A4 hydrolase in human skin. J Invest Dermatol. 1994 Feb;102(2):253-7. PMID: 8106755 Our lysate ab30166 may be suitable as a negative control but I cannot guarantee you will not see some reactivity. For the cytochrome P450 enzymes, I suggest liver lysates. A positive control for ab4236 is human liver: ab29889. For ab13513 and ab3573, rat liver lysate ab29413 will be appropriate. I am reluctant to recommend a negative control since information regarding absence of expression is difficult to come by but since CP450 is associated with the ER, cytoplasmic fractions of liver lysates (which do not contain membrane) may be useful. For human liver, this would be ab29875, and for rat liver ab29409. You should also have a look at the references listed on the datasheets to see how other researchers have used the antibodies, though the references for the LTA4 are general and not specific to the antibody. I hope this helps. Please do not hesitate to contact us if you have any other questions.

      Read More

      Abcam Scientific Support

      Answered on Nov 27 2006

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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