Overview

  • Product name

    Anti-CYP2C9 antibody [EPR7340]
    See all CYP2C9 primary antibodies
  • Description

    Rabbit monoclonal [EPR7340] to CYP2C9
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, Flow Cytmore details
    Unsuitable for: IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CYP2C9 aa 450-550 (C terminal). The exact sequence is proprietary.

  • Positive control

    • Human fetal liver and HepG2 whole cell lysate (ab7900), HepG2 cells
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     This product was previously labelled as Cytochrome P450 2C9

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab150364 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 56 kDa).
ICC/IF 1/100 - 1/250.
Flow Cyt 1/1000 - 1/10000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

  • Application notes
    Is unsuitable for IHC-P or IP.
  • Target

    • Function

      Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This enzyme contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S-warfarin, diclofenac, phenytoin, tolbutamide and losartan.
    • Sequence similarities

      Belongs to the cytochrome P450 family.
    • Cellular localization

      Endoplasmic reticulum membrane. Microsome membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • (R)-limonene 6-monooxygenase antibody
      • (S)-limonene 6-monooxygenase antibody
      • (S)-limonene 7-monooxygenase antibody
      • CP2C9_HUMAN antibody
      • CPC9 antibody
      • CYP2C antibody
      • CYP2C10 antibody
      • CYP2C9 antibody
      • CYPIIC9 antibody
      • cytochrome P-450 S-mephenytoin 4-hydroxylase antibody
      • Cytochrome P-450MP antibody
      • Cytochrome P450 2C9 antibody
      • Cytochrome P450 MP-4 antibody
      • Cytochrome P450 MP-8 antibody
      • Cytochrome P450 PB-1 antibody
      • Cytochrome P450, family 2, subfamily C, polypeptide 9 antibody
      • Cytochrome p4502C9 antibody
      • flavoprotein-linked monooxygenase antibody
      • MGC149605 antibody
      • MGC88320 antibody
      • microsomal monooxygenase antibody
      • OTTHUMP00000020135 antibody
      • P450 MP antibody
      • P450 PB 1 antibody
      • P450 PB1 antibody
      • P450IIC9 antibody
      • P450MP antibody
      • S mephenytoin 4 hydroxylase antibody
      • S-mephenytoin 4-hydroxylase antibody
      • xenobiotic monooxygenase antibody
      see all

    Images

    • All lanes : Anti-CYP2C9 antibody [EPR7340] (ab150364) at 1/1000 dilution

      Lane 1 : Human fetal liver cell lysate
      Lane 2 : HepG2 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 56 kDa
      Observed band size: 50 kDa
      why is the actual band size different from the predicted?

    • Overlay histogram showing HepG2 cells stained with ab150364 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab150364, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
    • Immunofluorescent analysis of HepG2 cells labelling CYP2C9 with ab150364 at a dilution of 1/100.

    References

    This product has been referenced in:

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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