Overview

  • Product name

  • Description

    Rabbit polyclonal to CYP2J2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment corresponding to Human CYP2J2 aa 213-472.
    Database link: P51589

  • Positive control

    • Human breast cancer tissue; H1299, A431, HeLa, HepG2 whole cell lysate (ab7900)
  • General notes

     This product was previously labelled as Cytochrome p450 2J2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab151996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.
  • Tissue specificity

    Highly expressed in heart, present at lower levels in liver, ileum, jejunum, colon, and kidney.
  • Sequence similarities

    Belongs to the cytochrome P450 family.
  • Cellular localization

    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Arachidonic acid epoxygenase antibody
    • CP2J2_HUMAN antibody
    • CPJ2 antibody
    • CYP2J2 antibody
    • CYPIIJ2 antibody
    • Cytochrome P450 2J2 antibody
    • Cytochrome P450 family 2 subfamily J polypeptide 2 antibody
    • Cytochrome P450 subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2 antibody
    • Cytochrome P450 subfamily IIJ polypeptide 2 antibody
    • Flavoprotein linked monooxygenase antibody
    • Microsomal monooxygenase antibody
    • OTTHUMP00000010550 antibody
    see all

Images

  • All lanes : Anti-CYP2J2 antibody (ab151996) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 58 kDa



    7.5% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling CYP2J2 with ab151996 at 1/500 dilution.

References

ab151996 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293T)
Permeabilization
Yes - Triton-X
Specification
HEK293T
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Fixative
Paraformaldehyde

Kavin Abelak

Verified customer

Submitted Apr 12 2016

Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (breast lines cells)
Total protein in input
2000 µg
Immuno-precipitation step
Protein A
Specification
breast lines cells

Abcam user community

Verified customer

Submitted Oct 06 2015

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