Overview

  • Product name

  • Description

    Rabbit polyclonal to CYP2J2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Macaque monkey
  • Immunogen

    Synthetic peptide corresponding to Human CYP2J2 aa 250-350 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab88195)

  • Positive control

    • This antibody gave a positive signal in HEK293 whole cell lysate and in the following tissue lysates: Human heart; Human liver; Human kidney; Human colon.
  • General notes

     This product was previously labelled as Cytochrome p450 2J2

     

Properties

Applications

Our Abpromise guarantee covers the use of ab76176 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).

Target

  • Function

    This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools.
  • Tissue specificity

    Highly expressed in heart, present at lower levels in liver, ileum, jejunum, colon, and kidney.
  • Sequence similarities

    Belongs to the cytochrome P450 family.
  • Cellular localization

    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • Arachidonic acid epoxygenase antibody
    • CP2J2_HUMAN antibody
    • CPJ2 antibody
    • CYP2J2 antibody
    • CYPIIJ2 antibody
    • Cytochrome P450 2J2 antibody
    • Cytochrome P450 family 2 subfamily J polypeptide 2 antibody
    • Cytochrome P450 subfamily IIJ (arachidonic acid epoxygenase) polypeptide 2 antibody
    • Cytochrome P450 subfamily IIJ polypeptide 2 antibody
    • Flavoprotein linked monooxygenase antibody
    • Microsomal monooxygenase antibody
    • OTTHUMP00000010550 antibody
    see all

Images

  • All lanes : Anti-CYP2J2 antibody (ab76176) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human liver tissue lysate - total protein (ab29889)
    Lane 3 : Human kidney tissue lysate - total protein (ab30203)
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size: 57 kDa
    Observed band size: 57 kDa
    Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 5 minutes

References

This product has been referenced in:

  • Drolet B  et al. Altered Protein Expression of Cardiac CYP2J and Hepatic CYP2C, CYP4A, and CYP4F in a Mouse Model of Type II Diabetes-A Link in the Onset and Development of Cardiovascular Disease? Pharmaceutics 9:N/A (2017). Read more (PubMed: 29023376) »
See 1 Publication for this product

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