Anti-CYP2J2 antibody (ab76176)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-CYP2J2 antibody
See all CYP2J2 primary antibodies -
Description
Rabbit polyclonal to CYP2J2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Macaque monkey
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Immunogen
Synthetic peptide corresponding to Human CYP2J2 aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available asab88195) -
Positive control
- This antibody gave a positive signal in HEK293 whole cell lysate and in the following tissue lysates: Human heart; Human liver; Human kidney; Human colon.
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General notes
This product was previously labelled as Cytochrome p450 2J2
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab76176 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
Target
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Function
This enzyme metabolizes arachidonic acid predominantly via a NADPH-dependent olefin epoxidation to all four regioisomeric cis-epoxyeicosatrienoic acids. One of the predominant enzymes responsible for the epoxidation of endogenous cardiac arachidonic acid pools. -
Tissue specificity
Highly expressed in heart, present at lower levels in liver, ileum, jejunum, colon, and kidney. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1573 Human
- Omim: 601258 Human
- SwissProt: P51589 Human
- Unigene: 152096 Human
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Alternative names
- Arachidonic acid epoxygenase antibody
- CP2J2_HUMAN antibody
- CPJ2 antibody
see all
Images
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Western blot - Anti-CYP2J2 antibody (ab76176)All lanes : Anti-CYP2J2 antibody (ab76176) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : Human liver tissue lysate - total protein (ab29889)
Lane 3 : Human kidney tissue lysate - total protein (ab30203)
Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Additional bands at: 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 5 minutes
Protocols
Datasheets and documents
References
This product has been referenced in:
- Drolet B et al. Altered Protein Expression of Cardiac CYP2J and Hepatic CYP2C, CYP4A, and CYP4F in a Mouse Model of Type II Diabetes-A Link in the Onset and Development of Cardiovascular Disease? Pharmaceutics 9:N/A (2017). Read more (PubMed: 29023376) »
