Key features and details
- Rabbit polyclonal to CYP2R1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CYP2R1 antibody
See all CYP2R1 primary antibodies
DescriptionRabbit polyclonal to CYP2R1
Tested applicationsSuitable for: IHC-P, ICC/IF, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Monkey
Recombinant fragment corresponding to a region within amino acids 83 and 360 of Human CYP2R1.
- HeLa whole cell lysate; methanol-fixed HeLa cells; FaDu xenograft.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
Our Abpromise guarantee covers the use of ab137634 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||1/100 - 1/1000.|
|WB||1/500 - 1/3000. Predicted molecular weight: 57 kDa.|
FunctionHas a D-25-hydroxylase activity on both forms of vitamin D, vitamin D(2) and D(3).
Involvement in diseaseDefects in CYP2R1 are the cause of rickets vitamin D-dependent type 1B (VDDR1B) [MIM:600081]; also known as pseudovitamin D(3) deficiency rickets due to 25-hydroxylase deficiency. A disorder caused by a selective deficiency of the active form of vitamin D (1,25-dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets. The patients sera have low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activityand low levels of 25-hydroxyvitamin D.
Sequence similaritiesBelongs to the cytochrome P450 family.
Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
- Information by UniProt
- CP2R1_HUMAN antibody
- CYP2R1 antibody
- Cytochrome P450 2R1 antibody
Anti-CYP2R1 antibody (ab137634) at 1/3000 dilution + HeLa whole cell lysate at 30 µg
Predicted band size: 57 kDa
7.5% SDS Page
Immunohistochemical analysis of paraffin-embedded FaDu xenograft, labelling CYP2R1 using ab137634 at 1/500 dilution.
Immunofluorescence analysis of methanol-fixed HeLa cells, labelling CYP2R1 using ab137634 at 1/200 dilution. lower panel represents cells costained with Hoechst 33342.
ab137634 has been referenced in 2 publications.
- Bukuroshi P et al. Strategies and limitations associated with in vitro characterization of vitamin D receptor activators. Biochem Pharmacol 155:547-561 (2018). PubMed: 30028992
- Bergadà L et al. Role of local bioactivation of vitamin D by CYP27A1 and CYP2R1 in the control of cell growth in normal endometrium and endometrial carcinoma. Lab Invest 94:608-22 (2014). PubMed: 24732451