Overview

  • Product name

  • Description

    Rabbit polyclonal to CYP2R1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IF, WBmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Monkey
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 83 and 360 of Human CYP2R1.

  • Positive control

    • HeLa whole cell lysate; methanol-fixed HeLa cells; FaDu xenograft.

Properties

Applications

Our Abpromise guarantee covers the use of ab137634 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.
WB 1/500 - 1/3000. Predicted molecular weight: 57 kDa.

Target

  • Function

    Has a D-25-hydroxylase activity on both forms of vitamin D, vitamin D(2) and D(3).
  • Involvement in disease

    Defects in CYP2R1 are the cause of rickets vitamin D-dependent type 1B (VDDR1B) [MIM:600081]; also known as pseudovitamin D(3) deficiency rickets due to 25-hydroxylase deficiency. A disorder caused by a selective deficiency of the active form of vitamin D (1,25-dihydroxyvitamin D3) and resulting in defective bone mineralization and clinical features of rickets. The patients sera have low calcium concentrations, low phosphate concentrations, elevated alkaline phosphatase activityand low levels of 25-hydroxyvitamin D.
  • Sequence similarities

    Belongs to the cytochrome P450 family.
  • Cellular localization

    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • CP2R1_HUMAN antibody
    • CYP2R1 antibody
    • Cytochrome P450 2R1 antibody
    • Cytochrome P450 family 2 R1 antibody
    • Cytochrome P450 family 2 subfamily R polypeptide 1 antibody
    • EC 1.14.13.15 antibody
    • MGC4663 antibody
    • Vitamin D 25 hydroxylase antibody
    • Vitamin D 25-hydroxylase antibody
    see all

Images

  • Anti-CYP2R1 antibody (ab137634) at 1/3000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size: 57 kDa



    7.5% SDS Page
  • Immunohistochemical analysis of paraffin-embedded FaDu xenograft, labelling CYP2R1 using ab137634 at 1/500 dilution.
  • Immunofluorescence analysis of methanol-fixed HeLa cells, labelling CYP2R1 using ab137634 at 1/200 dilution. lower panel represents cells costained with Hoechst 33342.

References

This product has been referenced in:

  • Bukuroshi P  et al. Strategies and limitations associated with in vitro characterization of vitamin D receptor activators. Biochem Pharmacol 155:547-561 (2018). Read more (PubMed: 30028992) »
  • Bergadà L  et al. Role of local bioactivation of vitamin D by CYP27A1 and CYP2R1 in the control of cell growth in normal endometrium and endometrial carcinoma. Lab Invest 94:608-22 (2014). Read more (PubMed: 24732451) »
See all 2 Publications for this product

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