Anti-Cyp3a1 antibody [P6] (ab22724)
Key features and details
- Mouse monoclonal [P6] to Cyp3a1
- Suitable for: ELISA, Flow Cyt, WB
- Reacts with: Mouse, Rat, Human, Pig
- Isotype: IgG1
Overview
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Product name
Anti-Cyp3a1 antibody [P6]
See all Cyp3a1 primary antibodies -
Description
Mouse monoclonal [P6] to Cyp3a1 -
Host species
Mouse -
Tested applications
Suitable for: ELISA, Flow Cyt, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Pig -
Immunogen
Full length protein corresponding to Rat Cyp3a1.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Protein A/G purified -
Clonality
Monoclonal -
Clone number
P6 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22724 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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Flow Cyt |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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WB | (2) |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
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WB
Use at an assay dependent concentration. |
Target
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Relevance
In animals, P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs. The four major families involved in drug metabolism are CYP 1,2,3, and 4. Cytochrome P450 3A4 [human-3A1 Rat] is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin. -
Cellular localization
Endoplasmic reticulum, membrane bound. -
Database links
- Entrez Gene: 107814 Mouse
- Entrez Gene: 25642 Rat
- SwissProt: P04800 Rat
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Alternative names
- CYP3A1 antibody
- cytochrome P450, family 3, subfamily a, polypeptide 1 antibody
Images
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Overlay histogram showing HepG2 cells stained with ab22724 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22724, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Datasheets and documents
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Datasheet download
References (7)
ab22724 has been referenced in 7 publications.
- Yuan K et al. The Effect of Vascular Endothelial Growth Factor on Bone Marrow Mesenchymal Stem Cell Engraftment in Rat Fibrotic Liver upon Transplantation. Stem Cells Int 2019:5310202 (2019). PubMed: 31885614
- Gabbia D et al. Pregnane X receptor and constitutive androstane receptor modulate differently CYP3A-mediated metabolism in early- and late-stage cholestasis. World J Gastroenterol 23:7519-7530 (2017). WB ; Rat . PubMed: 29204052
- Qin Y et al. Comparison of Pharmacokinetics and Tissue Distribution Kinetics of Roxithromycin and Expression of CYP 3A1 between Pregnant Mice and Foetuses. Basic Clin Pharmacol Toxicol 120:146-151 (2017). WB ; Mouse . PubMed: 27611991
- Chow EC et al. Functional Integrity of the Chimeric (Humanized) Mouse Liver: Enzyme Zonation, Physiologic Spaces, and Hepatic Enzymes and Transporters. Drug Metab Dispos 44:1524-35 (2016). PubMed: 27342868
- Probert PM et al. Utility of B-13 progenitor-derived hepatocytes in hepatotoxicity and genotoxicity studies. Toxicol Sci 137:350-70 (2014). WB ; Human . PubMed: 24235770
- Hu N et al. Opposite effect of diabetes mellitus induced by streptozotocin on oral and intravenous pharmacokinetics of verapamil in rats. Drug Metab Dispos 39:419-25 (2011). WB ; Rat . PubMed: 21135265
- Matuskova Z et al. Influence of probiotics on rat liver biotransformation enzymes. Neuro Endocrinol Lett 30 Suppl 1:41-5 (2009). WB ; Rat . PubMed: 20027143