For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome

Hello. We're improving abcam.com and we'd welcome your feedback.

Hello. We're improving abcam.com and we'd welcome your feedback.

Infomation icon

We haven't added this to the BETA yet

New BETA website

New BETA website

Hello. We're improving abcam.com and we'd welcome your feedback.

Take a look at our BETA site and see what we’ve done so far.

Switch on our new BETA site

Now available

Search and browse selected products

  • A selection of primary antibodies

Purchase these through your usual distributor

In the coming months

  • Additional product types
  • Supporting content
  • Sign in to your account
  • Purchase online
United States
Your country/region is currently set to:

If incorrect, please enter your country/region into the box below, to view site information related to your country/region.

Call (888) 77-ABCAM (22226) or contact us
Need help? Contact us

  • My account
  • Sign out
Sign in or Register with us

Welcome

Sign in or

Don't have an account?

Register with us
My basket
Quick order
Abcam homepage

  • Research Products
    By product type
    Primary antibodies
    Secondary antibodies
    ELISA and Matched Antibody Pair Kits
    Cell and tissue imaging tools
    Cellular and biochemical assays
    Proteins and Peptides
    By product type
    Proteomics tools
    Agonists, activators, antagonists and inhibitors
    Cell lines and Lysates
    Multiplex miRNA assays
    Multiplex Assays
    By research area
    Cancer
    Cardiovascular
    Cell Biology
    Epigenetics
    Metabolism
    Developmental Biology
    By research area
    Immunology
    Microbiology
    Neuroscience
    Signal Transduction
    Stem Cells
  • Customized Products & Partnerships
    Customized Products & Partnerships

    Customized products and commercial partnerships to accelerate your diagnostic and therapeutic programs.

    Customized products

    Partner with us

  • Support
    Support hub

    Access advice and support for any research roadblock

    View support hub

    Protocols

    Your experiments laid out step by step

    View protocols

  • Events
    • Conference calendar
    • Cancer
    • Cardiovascular
    • Epigenetics & Nuclear signaling
    • Immunology
    • Neuroscience
    • Stem cells
    • Tradeshows
    • Scientific webinars
    Keep up to date with the latest events

    Full event breakdown with abstracts, speakers, registration and more

    View global event calendar

  • Pathways
    Cell signalling pathways

    View all pathways

    View all interactive pathways

Explore the power of knock-out cell lines for your research

  1. Link

    cyp3a1-antibody-p6-ab22724.pdf

  1. Send me a copy of this email
    I agree to the terms and conditions.
Cardiovascular Lipids / Lipoproteins Lipid Metabolism Cytochromes
Share by email

Anti-Cyp3a1 antibody [P6] (ab22724)

  • Datasheet
Reviews (2)Q&A (4)References (7)

Product price, shipping and contact information

Currently unavailable

Sorry, we can't display this right now.

Please contact us to place your order, or try again later.

 

Loading size & price…

 

Shipping and order information

Shipping info

Promotion Information

Abpromise

Guaranteed product quality, expert customer support.

Find out more.

Flow Cytometry - Anti-Cyp3a1 antibody [P6] (ab22724)

    Key features and details

    • Mouse monoclonal [P6] to Cyp3a1
    • Suitable for: ELISA, Flow Cyt, WB
    • Reacts with: Mouse, Rat, Human, Pig
    • Isotype: IgG1

    You may also be interested in

    Primary
    Product image
    Anti-Cytochrome P450 3A4/CYP3A4 antibody [EPR6202] (ab124921)
    Secondary
    Product image
    Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    Primary
    Product image
    Anti-CYP2C11 antibody (ab3571)

    View more associated products

    Overview

    • Product name

      Anti-Cyp3a1 antibody [P6]
      See all Cyp3a1 primary antibodies
    • Description

      Mouse monoclonal [P6] to Cyp3a1
    • Host species

      Mouse
    • Tested applications

      Suitable for: ELISA, Flow Cyt, WBmore details
    • Species reactivity

      Reacts with: Mouse, Rat, Human, Pig
    • Immunogen

      Full length protein corresponding to Rat Cyp3a1.

    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
    • Storage buffer

      Constituent: PBS
    • Concentration information loading...
    • Purity

      Protein A/G purified
    • Clonality

      Monoclonal
    • Clone number

      P6
    • Isotype

      IgG1
    • Research areas

      • Cardiovascular
      • Lipids / Lipoproteins
      • Lipid Metabolism
      • Cytochromes
      • Signal Transduction
      • Metabolism
      • Drug metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipases
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Drug metabolism
      • Metabolism
      • Pathways and Processes
      • Mitochondrial Metabolism
      • Cytochromes
      • Metabolism
      • Types of disease
      • Cancer

    Associated products

    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
      • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
      • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab22724 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    Use at an assay dependent concentration.
    Flow Cyt
    Use 1-2µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    WB (2)
    Use at an assay dependent concentration.
    Notes
    ELISA
    Use at an assay dependent concentration.
    Flow Cyt
    Use 1-2µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    WB
    Use at an assay dependent concentration.

    Target

    • Relevance

      In animals, P-450 enzymes serve two major functions: (1) biosynthesis of steroids, fatty acids, and bile acids; (2) metabolism of endogenous and a wide variety of exogenous substrates, such as toxins and drugs. The four major families involved in drug metabolism are CYP 1,2,3, and 4. Cytochrome P450 3A4 [human-3A1 Rat] is abundantly expressed in liver and small intestine and is inducible by barbiturates, glucocorticoids and rifampicin.
    • Cellular localization

      Endoplasmic reticulum, membrane bound.
    • Database links

      • Entrez Gene: 107814 Mouse
      • Entrez Gene: 25642 Rat
      • SwissProt: P04800 Rat
      • Alternative names

        • CYP3A1 antibody
        • cytochrome P450, family 3, subfamily a, polypeptide 1 antibody

      Images

      • Flow Cytometry - Anti-Cyp3a1 antibody [P6] (ab22724)
        Flow Cytometry - Anti-Cyp3a1 antibody [P6] (ab22724)

        Overlay histogram showing HepG2 cells stained with ab22724 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab22724, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      Protocols

      • Flow cytometry protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet download

        Download

      References (7)

      Publishing research using ab22724? Please let us know so that we can cite the reference in this datasheet.

      ab22724 has been referenced in 7 publications.

      • Yuan K  et al. The Effect of Vascular Endothelial Growth Factor on Bone Marrow Mesenchymal Stem Cell Engraftment in Rat Fibrotic Liver upon Transplantation. Stem Cells Int 2019:5310202 (2019). PubMed: 31885614
      • Gabbia D  et al. Pregnane X receptor and constitutive androstane receptor modulate differently CYP3A-mediated metabolism in early- and late-stage cholestasis. World J Gastroenterol 23:7519-7530 (2017). WB ; Rat . PubMed: 29204052
      • Qin Y  et al. Comparison of Pharmacokinetics and Tissue Distribution Kinetics of Roxithromycin and Expression of CYP 3A1 between Pregnant Mice and Foetuses. Basic Clin Pharmacol Toxicol 120:146-151 (2017). WB ; Mouse . PubMed: 27611991
      • Chow EC  et al. Functional Integrity of the Chimeric (Humanized) Mouse Liver: Enzyme Zonation, Physiologic Spaces, and Hepatic Enzymes and Transporters. Drug Metab Dispos 44:1524-35 (2016). PubMed: 27342868
      • Probert PM  et al. Utility of B-13 progenitor-derived hepatocytes in hepatotoxicity and genotoxicity studies. Toxicol Sci 137:350-70 (2014). WB ; Human . PubMed: 24235770
      • Hu N  et al. Opposite effect of diabetes mellitus induced by streptozotocin on oral and intravenous pharmacokinetics of verapamil in rats. Drug Metab Dispos 39:419-25 (2011). WB ; Rat . PubMed: 21135265
      • Matuskova Z  et al. Influence of probiotics on rat liver biotransformation enzymes. Neuro Endocrinol Lett 30 Suppl 1:41-5 (2009). WB ; Rat . PubMed: 20027143

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-6 of 6 Abreviews or Q&A

      Western blot abreview for Anti-Cytochrome P450 3A1 antibody [P6]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Pig Tissue lysate - other (liver microsomal protein)
      Loading amount
      20 µg
      Specification
      liver microsomal protein
      Gel Running Conditions
      Non-reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Ying Chen

      Verified customer

      Submitted Jun 15 2007

      Western blot abreview for Anti-Cytochrome P450 3A1 antibody [P6]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Rat Tissue lysate - other (liver)
      Loading amount
      20 µg
      Specification
      liver
      Gel Running Conditions
      Non-reduced Denaturing
      Blocking step
      Milk as blocking agent for 14 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More
      The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

      DR. Ying Chen

      Verified customer

      Submitted Jun 11 2007

      Question

      Thank you for your reply. Here you find the answers to your questions:

      1. The order number is ###

      2. In the attached file you can see some images of the blots we have. For each image you can find blot conditions.

      3. We generally use milk solution to block the aspecific sites and we’ve never had problems. In our case, I guess the problem is not a high aspecific signal (which we could see only in our samples with a big increase of the image contrast) but the absence of bands, in our samples and also in the positive controls. Anyway, in your site I found that another research group performed very similar experiments using milk as blocking agent (reference: https://www.abcam.com/Cyp3a1-antibody-P6-ab22724.html&tab=abreviews&intabreviewid=7693).

      4. The microsomal fractions are prepared according to a method of differential centrifugation as described in:

      - Floreani M, Gabbia D, Barbierato M, DE Martin S, Palatini P., Drug Metab Pharmacokinet. 2012 (in press). Differential inducing effect of benzo[a]pyrene on gene expression and enzyme activity of cytochromes P450 1A1 and 1A2 in Sprague-Dawley and Wistar rats.

      Recombinant rat CYP3A1 microsomes are purchased from BDGentest and are used as positive control, since they are microsomes which express almost exclusively our protein of interest. Using this type of positive control usually permit to avoid the risk of unspecific signal, which is common with whole cell lysates.

      Both samples of microsomes were prepared with Laemmli loading buffer (4% SDS, 20% glycerol, 0.004% bromophenol blue, 0.125 M Tris HCl) even with or without 10% b-mercaptoethanol, and then boiled for 5 minutes.

      5. We used two different secondary antibodies that have been successfully tested with other primary antibodies (anti b-actin, anti cyp1A1/1A2 and anti AhR antibodies-reference: Floreani M, Gabbia D, Barbierato M, De Martin S, Palatini P., Drug Metab Pharmacokinet. 2012, Differential inducing effect of benzo[a]pyrene on gene expression and enzyme activity of cytochromes P450 1A1 and 1A2 in Sprague-Dawley and Wistar rats.)

      6. The antibody was used the first time the day of arrival and then stored as described in the datasheet.

      I hope my answers are clear and exhaustive. Please let me know if you have further suggestions. Otherwise, since I have seen two polyclonal antibodies anti cyp3A1 (ab22733, AntiCytochrome P450 3A1 antibody Rabbit polyclonal; and ab22732, Anti-Cytochrome P450 3A1 antibody Sheep polyclonal), I wonder if you can provide us a credit note to try one of them. They look similar to the CYP3A2 one, that works very well in our samples and conditions.

      Looking forward to hearing from you,

      Best regards

      Read More

      Abcam community

      Verified customer

      Asked on Oct 17 2012

      Answer

      Thank you for your message and for providing this further information.

      I am sorry to hear the suggestions made have not improved the results on this occasion. I appreciate the time you have spent on these experiments and would be pleased to arrange a free of charge replacement or credit note in compensation.

      I look forward to hearing from you with details of how you would like to proceed.

      Read More

      Abcam Scientific Support

      Answered on Oct 17 2012

      Question

      Inquiry: We performed several western blots with this antibody following these conditions: -
      Rat liver microsomes: 50-20 or 10 ug - Recombinant rat CYP3A1 microsomes: 10-2.5-0.25-0.125 pmoles.
      -Blocking condition: 10% milk in TBST 1 h, RT
      - Luminata (Millipore) or ECL Plus (Amersham) as HRP conjugated detection systems, using a Versadoc (Biorad) detector
      - SDS-PAGE 8% PAA, blot on a nitrocellulose membrane. Protein samples were prepared in denaturing and reducing condition (beta-mercaptoethanol) and, subsequently, in denaturing and non-reducing conditions (as you described for this antibody).
      - Primary antibody: dilutions used: 1:2000, 1:1000, 1:250, 1:100 in 5% milk in TBST. Incubation was performed ON at 4 deg.
      -Secondary antibody: dilution used 1:2000, 1:5000 in 5% milk in TBST. Two different secondary antibodies were tested without any difference in results. Incubation was performed for 1,5 h, RT.
      -Wash steps: 3 times for 10 minutes after blocking, primary antibody and secondary antibody incubation.
      We performed the same experiments with Anti-CYP3A2 (ab22731), obtaining perfect results with primary antibody dilution of 1:2000 . With anti-CYP3A1 we unfortunately cannot see the band of the reference protein and the sample lanes are even without signal or loaded with unspecific staining.
      Looking forward to hearing from you, sincerely

      Read More

      Abcam community

      Verified customer

      Asked on Oct 15 2012

      Answer

      Thank you for taking the time to complete our questionnaire and contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

      The details you have kindly provided will enable us to investigate this case for you and this is also helpful in our records for monitoring of quality.

      I would like to reassure you that ab22724 is tested and covered by our 6 month guarantee for use in rat samples and WB. In the event that a product is not functioning in the applications cited on the product data sheet, we will be pleased to provide a credit note or free of charge replacement.

      Although the other antibody has worked well using this procedure, individual antibodies will often require optimization. I would like to offer some suggestions to help optimize the results from ab22724. I would also appreciate if you can confirm some details of the procedure.

      1. Please confirm the order number and date of purchase.

      2. Please confirm what the results were. Is it sometimes a clean blot with no bands, and sometimes a lot of bands depending on the dilution of antibody used? I would appreciate if you are able to provide some images which will help me to assess the results. The results from the different dilutions tried.

      3. I can recommend to try using BSA rather than milk to block, at 5%. Changing blocking agent can sometimes help to improve results.

      4. I would appreciate if you are able to describe how the microsomes were prepared?
      I can recommend it would be important to include a whole cell lystate (cells lysed in RIPA buffer) as a positive control.

      5. Have the current vials of secondary antibody been used successfully with other primary antibodies? What were the results of the no primary control?

      6. How was the antibody stored?

      I hope this information is helpful, thank you for your cooperation. Should the suggestions not improve the results, please do not hesitate to contact me again with the further requested details.

      Read More

      Abcam Scientific Support

      Answered on Oct 15 2012

      Question

      I'm a researcher interested in your products.
      I need some information on two specific primary antibodies (codes: ab22724 and ab125803). I have to detect the CYP3A family (in particular CYP3A1 and CYP3A2) in a WB experiment using a preparation of rat liver microsomes.
      I see in the datasheets that immunogen of your two products is the same (rat liver cytochrome P450 3A1) and I want to know what of two ab is better for my purpouse and what are the differences between them.
      Thank you for your kindly attention.
      Best regards.

      Read More

      Abcam community

      Verified customer

      Asked on Aug 24 2012

      Answer

      Thank you for your email.

      The product ab22724 has been tested with rat samples so it is fully guaranteed. Please check the Abcam abpromise guarantee policy; https://www.abcam.com/index.html?pageconfig=abpromise

      Product ab125803 is now unpublished, which means, it not available for sale anymore.

      I hope this information will be helpful. Should you have any question please do not hesitate to contact me.

      Read More

      Abcam Scientific Support

      Answered on Aug 24 2012

      Question

      Product code: 22724

      Inquiry: Does this antibody crossreact with rat cyp3A2 in western blot?

      Read More

      Abcam community

      Verified customer

      Asked on Aug 10 2012

      Answer

      Thank you for your enquiry.

      I can confirm that ab22724 Cytochrome P450 3A1 antibody [P6] has been tested for cross-reactivity against other CYP3A family members but no evidence was seen to show that it might recognize CYP3A2. I am sorry this has been sourced and tested externally so we regrettably have no data regarding this information from the originating scientist on this occasion.

      I hope this will be helpful. If you have any further questions, please do not hesitate to contact us.

      Read More

      Abcam Scientific Support

      Answered on Aug 10 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

      Get resources and offers direct to your inbox Sign up
      A-Z by research area
      • Cancer
      • Cardiovascular
      • Cell biology
      • Developmental biology
      • Epigenetics & Nuclear signaling
      • Immunology
      • Metabolism
      • Microbiology
      • Neuroscience
      • Signal transduction
      • Stem cells
      A-Z by product type
      • Primary antibodies
      • Secondary antibodies
      • Biochemicals
      • Isotype controls
      • Flow cytometry multi-color selector
      • Kits
      • Loading controls
      • Lysates
      • Peptides
      • Proteins
      • Slides
      • Tags and cell markers
      • Tools & Reagents
      Help & support
      • Support
      • Make an Inquiry
      • Protocols & troubleshooting
      • Placing an order
      • RabMAb products
      • Biochemical product FAQs
      • Training
      • Browse by Target
      Company
      • Corporate site
      • Investor relations
      • Company news
      • Careers
      • About us
      • Blog
      Events
      • Tradeshows
      • Conferences
      International websites
      • abcam.cn
      • abcam.co.jp

      Join with us

      • LinkedIn
      • facebook
      • Twitter
      • YouTube
      • Terms of sale
      • Website terms of use
      • Cookie policy
      • Privacy policy
      • Legal
      • Modern slavery statement
      © 1998-2022 Abcam plc. All rights reserved.