Anti-CYP3A43 antibody (ab155029)
Key features and details
- Rabbit polyclonal to CYP3A43
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CYP3A43 antibody
See all CYP3A43 primary antibodies -
Description
Rabbit polyclonal to CYP3A43 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Monkey -
Immunogen
Recombinant fragment corresponding to a region within amino acids 33-248 of Human CYP3A43 (UniProt ID: Q9HB55).
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Positive control
- HCT116, HepG2, HeLa and A549 whole cell lysate. HeLa cells. Human breast carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155029 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/3000. Predicted molecular weight: 58 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 58 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Relevance
CYP3A43 is a member of the cytochrome P450 superfamily of enzymes which are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP3A43 exhibits low testosterone 6 beta hydroxylase activity. -
Cellular localization
Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein. -
Database links
- Entrez Gene: 64816 Human
- Omim: 606534 Human
- SwissProt: Q9HB55 Human
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Alternative names
- Cytochrome P450 family 3 subfamily A polypeptide 43 antibody
- Cytochrome P450 polypeptide 43 antibody
- Cytochrome P450 subfamily IIIA polypeptide 43 antibody
see all
Images
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Anti-CYP3A43 antibody (ab155029) at 1/2000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 58 kDa
10% SDS PAGE -
Immunofluorescence analysis of methanol fixed HeLa cells, labeling CYP3A43 using ab155029 at 1/200 dilution. Lower image costained with Hoechst 33342.
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Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue, labeling CYP3A43 using ab155029 at 1/250 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (5)
ab155029 has been referenced in 5 publications.
- Wang L et al. MicroRNA-519c-3p promotes tumor growth and metastasis of hepatocellular carcinoma by targeting BTG3. Biomed Pharmacother 118:109267 (2019). PubMed: 31387005
- Reebye V et al. Gene activation of CEBPA using saRNA: preclinical studies of the first in human saRNA drug candidate for liver cancer. Oncogene 37:3216-3228 (2018). PubMed: 29511346
- Meng D et al. MicroRNA-645 targets urokinase plasminogen activator and decreases the invasive growth of MDA-MB-231 triple-negative breast cancer cells. Onco Targets Ther 11:7733-7743 (2018). PubMed: 30464522
- Li J et al. MicroRNA-140-3p enhances the sensitivity of hepatocellular carcinoma cells to sorafenib by targeting pregnenolone X receptor. Onco Targets Ther 11:5885-5894 (2018). PubMed: 30271172
- Richter M et al. Pancreatic progenitor-derived hepatocytes are viable and functional in a 3D high density bioreactor culture system. Toxicol Res (Camb) 5:278-290 (2016). PubMed: 30090344