Overview

  • Product name
  • Description
    Rabbit polyclonal to CYP3A43
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Monkey
  • Immunogen

    Recombinant fragment corresponding to a region within amino acids 33-248 of Human CYP3A43 (UniProt ID: Q9HB55).

  • Positive control
    • HCT116, HepG2, HeLa and A549 whole cell lysate. HeLa cells. Human breast carcinoma tissue.
  • General notes
    Keep as concentrated solution.

Properties

Applications

Our Abpromise guarantee covers the use of ab155029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 58 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Relevance
    CYP3A43 is a member of the cytochrome P450 superfamily of enzymes which are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP3A43 exhibits low testosterone 6 beta hydroxylase activity.
  • Cellular localization
    Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
  • Database links
  • Alternative names
    • Cytochrome P450 family 3 subfamily A polypeptide 43 antibody
    • Cytochrome P450 polypeptide 43 antibody
    • Cytochrome P450 subfamily IIIA polypeptide 43 antibody
    • MGC119315 antibody
    • MGC119316 antibody
    see all

Images

  • Anti-CYP3A43 antibody (ab155029) at 1/2000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 58 kDa



    10% SDS PAGE
  • Immunofluorescence analysis of methanol fixed HeLa cells, labeling CYP3A43 using ab155029 at 1/200 dilution. Lower image costained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue, labeling CYP3A43 using ab155029 at 1/250 dilution.

References

This product has been referenced in:
  • Reebye V  et al. Gene activation of CEBPA using saRNA: preclinical studies of the first in human saRNA drug candidate for liver cancer. Oncogene 37:3216-3228 (2018). Read more (PubMed: 29511346) »
  • Richter M  et al. Pancreatic progenitor-derived hepatocytes are viable and functional in a 3D high density bioreactor culture system. Toxicol Res (Camb) 5:278-290 (2016). Read more (PubMed: 30090344) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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