Overview

  • Product name

  • Description

    Rabbit polyclonal to CYP3A43
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment corresponding to Human CYP3A43 aa 244-503.
    Database link: Q9HB55

  • Positive control

    • WB: Mouse liver and skeletal muscle tissue lysate; Jurkat and HEK-293T whole cell lysate. IHC-P: Human liver cancer tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab230022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 58 kDa (predicted molecular weight: 58, 48, 28, 45 kDa).
IHC-P 1/20 - 1/200.

Target

  • Relevance

    CYP3A43 is a member of the cytochrome P450 superfamily of enzymes which are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP3A43 exhibits low testosterone 6 beta hydroxylase activity.
  • Cellular localization

    Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
  • Database links

  • Alternative names

    • Cytochrome P450 family 3 subfamily A polypeptide 43 antibody
    • Cytochrome P450 polypeptide 43 antibody
    • Cytochrome P450 subfamily IIIA polypeptide 43 antibody
    • MGC119315 antibody
    • MGC119316 antibody
    see all

Images

  • Paraffin-embedded human liver cancer tissue stained for CYP3A43 with ab230022 at 1/100 dilution in immunohistochemical analysis.

  • All lanes : Anti-CYP3A43 antibody (ab230022) at 1/1000 dilution

    Lane 1 : Mouse liver tissue lysate
    Lane 2 : Mouse skeletal muscle tissue lysate
    Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
    Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate

    Secondary
    All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution

    Developed using the ECL technique.

    Predicted band size: 58, 48, 28, 45 kDa
    Observed band size: 58 kDa
    why is the actual band size different from the predicted?

References

ab230022 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab230022.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up