Key features and details
- Rabbit polyclonal to CYP3A43
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-CYP3A43 antibody
See all CYP3A43 primary antibodies
DescriptionRabbit polyclonal to CYP3A43
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Recombinant fragment corresponding to Human CYP3A43 aa 244-503.
Database link: Q9HB55
- WB: Mouse liver and skeletal muscle tissue lysate; Jurkat and HEK-293T whole cell lysate. IHC-P: Human liver cancer tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230022 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 58 kDa (predicted molecular weight: 58, 48, 28, 45 kDa).|
|IHC-P||1/20 - 1/200.|
RelevanceCYP3A43 is a member of the cytochrome P450 superfamily of enzymes which are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. CYP3A43 exhibits low testosterone 6 beta hydroxylase activity.
Cellular localizationEndoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
- Cytochrome P450 family 3 subfamily A polypeptide 43 antibody
- Cytochrome P450 polypeptide 43 antibody
- Cytochrome P450 subfamily IIIA polypeptide 43 antibody
Paraffin-embedded human liver cancer tissue stained for CYP3A43 with ab230022 at 1/100 dilution in immunohistochemical analysis.
All lanes : Anti-CYP3A43 antibody (ab230022) at 1/1000 dilution
Lane 1 : Mouse liver tissue lysate
Lane 2 : Mouse skeletal muscle tissue lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 58, 48, 28, 45 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230022 has not yet been referenced specifically in any publications.