Anti-CYP3A5 antibody [F18 P3 B6] (ab56076)
Key features and details
- Mouse monoclonal [F18 P3 B6] to CYP3A5
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-CYP3A5 antibody [F18 P3 B6]
See all CYP3A5 primary antibodies -
Description
Mouse monoclonal [F18 P3 B6] to CYP3A5 -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide corresponding to Human CYP3A5 (C terminal).
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Positive control
- Oesophageal Squamous cell carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.08% Sodium azide
Constituent: PBS -
Concentration information loading...
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Clonality
Monoclonal -
Clone number
F18 P3 B6 -
Myeloma
Ag8.653 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab56076 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa).
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IHC-P |
Use a concentration of 1 - 5 µg/ml.
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Notes |
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WB
Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 57 kDa (predicted molecular weight: 57 kDa). |
IHC-P
Use a concentration of 1 - 5 µg/ml. |
Target
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Function
Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1577 Human
- Omim: 605325 Human
- SwissProt: P20815 Human
- Unigene: 695915 Human
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Alternative names
- Aryl hydrocarbon hydroxylase antibody
- CP35 antibody
- CP3A5_HUMAN antibody
see all
Images
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All lanes : Anti-CYP3A5 antibody [F18 P3 B6] (ab56076) at 2 µg/ml
Lane 1 : CYP3A4 recombinant
CYP450 protein
Lane 2 : CYP3A5 recombinant
CYP450 protein
Lane 3 : CYP3A7 recombinant
CYP450 protein
Predicted band size: 57 kDa
Observed band size: 57 kDa -
Lane 1 : Molecular weight markers
Lanes 2-10 : Anti-CYP3A5 antibody [F18 P3 B6] (ab56076) at 2 µg/ml
Lane 2 : CYP1A1 recombinant protein
Lane 3 : CYP2C18 recombinant protein
Lane 4 : CYP2C19 recombinant protein
Lane 5 : CYP2D6 recombinant protein
Lane 6 : CYP4F2 recombinant protein
Lane 7 : CYP4F3 recombinant protein
Lane 8 : CYP3A5 recombinant protein
Lane 9 : CYP3A7 recombinant protein
Lane 10 : Negative control
Predicted band size: 57 kDa
Observed band size: 57 kDa -
Oesophageal squamous cell carcinoma tissue stained with ab56076 at 5 µg/ml.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab56076 has been referenced in 1 publication.
- Yan F et al. A dual functional probe for assessing human CYP450 3A5 and 3A enzymes bioactivities. Future Med Chem 11:2891-2903 (2019). PubMed: 31702381