Key features and details
- Rabbit polyclonal to CYP4B1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-CYP4B1 antibody
See all CYP4B1 primary antibodies
DescriptionRabbit polyclonal to CYP4B1
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
- IHC-P: Human nasopharynx tissue. WB: CYP4B1 over-expression HEK-293T cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS
Concentration information loading...
PurityImmunogen affinity purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
Our Abpromise guarantee covers the use of ab224062 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 59 kDa.|
|IHC-P||1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionCytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
Tissue specificityDetected in the liver and lung (at protein level).
Sequence similaritiesBelongs to the cytochrome P450 family.
Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
- Information by UniProt
- CP4B1_HUMAN antibody
- CYP4B1 antibody
- CYPIVB1 antibody
All lanes : Anti-CYP4B1 antibody (ab224062) at 1/100 dilution
Lane 1 : Negative control (vector only transfected HEK-293T lysate)
Lane 2 : CYP4B1 (with C-terminal myc-DDK tag, ~3.1 kDa) over-expression HEK-293T cell lysate
Predicted band size: 59 kDa
Paraffin embedded human nasopharynx tissue stained for CYP4B1 with ab224062 (1/20 dilution) in immunohistochemical analysis.
ab224062 has not yet been referenced specifically in any publications.