Anti-CYP4F2 antibody - N-terminal (ab230709)
Key features and details
- Rabbit polyclonal to CYP4F2 - N-terminal
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-CYP4F2 antibody - N-terminal
See all CYP4F2 primary antibodies -
Description
Rabbit polyclonal to CYP4F2 - N-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide corresponding to Human CYP4F2 (N terminal) conjugated to keyhole limpet haemocyanin.
Database link: P78329 -
Positive control
- IHC-P: Human small intestine tissue. WB: A549 and HepG2 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.01% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab230709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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WB |
1/500 - 1/1000. Predicted molecular weight: 60 kDa.
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Notes |
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IHC-P
1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Predicted molecular weight: 60 kDa. |
Target
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Relevance
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. -
Cellular localization
Endoplasmic reticulum; endoplasmic reticulum membrane; peripheral membrane protein. -
Database links
- Entrez Gene: 8529 Human
- Entrez Gene: 56266 Rat
- Omim: 604426 Human
- SwissProt: P78329 Human
- SwissProt: P33274 Rat
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Alternative names
- Cyp4f14 antibody
- CYPIVF1 antibody
- Cytochrome P450 LTB omega antibody
see all
Images
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Formalin-fixed, paraffin-embedded human small intestine tissue stained for CYP4F2 using ab230709 at 1/100 dilution in immunohistochemical analysis.
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All lanes : Anti-CYP4F2 antibody - N-terminal (ab230709) at 1/500 dilution
Lane 1 : A549 (Human lung carcinoma cell line) whole cell lysate
Lane 2 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate
Predicted band size: 60 kDa
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab230709 has not yet been referenced specifically in any publications.