Anti-CYP7B1 antibody (ab175889)
- Datasheet
- References
- Protocols
Overview
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Product nameAnti-CYP7B1 antibody
See all CYP7B1 primary antibodies -
DescriptionRabbit polyclonal to CYP7B1
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
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Species reactivityReacts with: Human
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Immunogen
Synthetic peptide within Human CYP7B1 aa 259-289 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: O75881 -
Positive control
- HeLa whole cell lysate (ab150035); HeLa cells; Human brain tissue.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.09% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
PurityAmmonium Sulphate Precipitation
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab175889 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| WB | 1/1000. Predicted molecular weight: 58 kDa. | |
| ICC/IF | 1/10 - 1/50. | |
| Flow Cyt | 1/10 - 1/50. ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Tissue specificityBrain, testis, ovary, prostate, liver, colon, kidney, and small intestine.
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PathwayLipid metabolism; bile acid biosynthesis.
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Involvement in diseaseDefects in CYP7B1 are the cause of spastic paraplegia autosomal recessive type 5A (SPG5A) [MIM:270800]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
Defects in CYP7B1 are the cause of congenital bile acid synthesis defect type 3 (CBAS3) [MIM:613812]. Clinical features include severe cholestasis, cirrhosis and liver synthetic failure. Hepatic microsomal oxysterol 7-alpha-hydroxylase activity is undetectable. -
Sequence similaritiesBelongs to the cytochrome P450 family.
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Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
- Information by UniProt
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Database links
- Entrez Gene: 9420 Human
- Omim: 603711 Human
- SwissProt: O75881 Human
- Unigene: 667720 Human
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Alternative names
- 25 hydroxycholesterol 7 alpha hydroxylase antibody
- 25-hydroxycholesterol 7-alpha-hydroxylase antibody
- CP7B antibody
see all
Images
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Western blot - Anti-CYP7B1 antibody (ab175889)Anti-CYP7B1 antibody (ab175889) at 1/1000 dilution + HeLa cell lysate at 35 µg
Predicted band size: 58 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYP7B1 antibody (ab175889)Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human brain tissue labeling CYP7B1 with ab175889 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.
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Flow Cytometry - Anti-CYP7B1 antibody (ab175889)Flow Cytometric analysis of HeLa cells labeling CYP7B1 with ab175889 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-CYP7B1 antibody (ab175889)Immunofluorescent analysis of HeLa cells labeling CYP7B1 with ab175889 at 1/10 dilution followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Protocols
Datasheets and documents
References
ab175889 has not yet been referenced specifically in any publications.
