Overview

  • Product name
  • Description
    Rabbit polyclonal to CYP7B1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CYP7B1 aa 259-289 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: O75881

  • Positive control
    • HeLa cell lysate; HeLa cells; Human brain tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab175889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 58 kDa.
ICC/IF 1/10 - 1/50.
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Tissue specificity
    Brain, testis, ovary, prostate, liver, colon, kidney, and small intestine.
  • Pathway
    Lipid metabolism; bile acid biosynthesis.
  • Involvement in disease
    Defects in CYP7B1 are the cause of spastic paraplegia autosomal recessive type 5A (SPG5A) [MIM:270800]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
    Defects in CYP7B1 are the cause of congenital bile acid synthesis defect type 3 (CBAS3) [MIM:613812]. Clinical features include severe cholestasis, cirrhosis and liver synthetic failure. Hepatic microsomal oxysterol 7-alpha-hydroxylase activity is undetectable.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 25 hydroxycholesterol 7 alpha hydroxylase antibody
    • 25-hydroxycholesterol 7-alpha-hydroxylase antibody
    • CP7B antibody
    • CP7B1_HUMAN antibody
    • Cyp7b1 antibody
    • Cytochrome P450 7B1 antibody
    • Cytochrome P450 family 7 subfamily B polypeptide 1 antibody
    • Cytochrome P450 subfamily VIIB polypeptide 1 antibody
    • Oxysterol 7-alpha-hydroxylase antibody
    • Oxysterol 7alpha hydroxylase antibody
    see all

Images

  • Anti-CYP7B1 antibody (ab175889) at 1/1000 dilution + HeLa cell lysate at 35 µg

    Predicted band size: 58 kDa

  • Immunohistochemical analysis of Formalin-fixed, Paraffin-embedded Human brain tissue labeling CYP7B1 with ab175889 at 1/50 dilution followed by peroxidase-conjugated secondary antibody and DAB staining.

  • Flow Cytometric analysis of HeLa cells labeling CYP7B1 with ab175889 at 1/10 dilution (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

  • Immunofluorescent analysis of HeLa cells labeling CYP7B1 with ab175889 at 1/10 dilution followed by Alexa Fluor® 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

References

ab175889 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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