Key features and details
- Goat polyclonal to CYP7B1
- Suitable for: IHC-P, WB
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipid metabolism
Our Abpromise guarantee covers the use of ab19043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 3 - 6 µg/ml.|
|WB||Use at an assay dependent concentration. PubMed: 19729835
A 1 hour primary incubation is recommended for this product.
Tissue specificityBrain, testis, ovary, prostate, liver, colon, kidney, and small intestine.
PathwayLipid metabolism; bile acid biosynthesis.
Involvement in diseaseDefects in CYP7B1 are the cause of spastic paraplegia autosomal recessive type 5A (SPG5A) [MIM:270800]. Spastic paraplegia is a neurodegenerative disorder characterized by a slow, gradual, progressive weakness and spasticity of the lower limbs. Rate of progression and the severity of symptoms are quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body.
Defects in CYP7B1 are the cause of congenital bile acid synthesis defect type 3 (CBAS3) [MIM:613812]. Clinical features include severe cholestasis, cirrhosis and liver synthetic failure. Hepatic microsomal oxysterol 7-alpha-hydroxylase activity is undetectable.
Sequence similaritiesBelongs to the cytochrome P450 family.
Cellular localizationEndoplasmic reticulum membrane. Microsome membrane.
- Information by UniProt
- 25 hydroxycholesterol 7 alpha hydroxylase antibody
- 25-hydroxycholesterol 7-alpha-hydroxylase antibody
- CP7B antibody
ab19043 at 4µg/ml, staining CYP7B1 in Human Breast tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Image shows textured cytoplasm staining in ductal epithelial cells. Steamed antigen retrieval with pH 6 citrate buffer was performed, and HRP-staining was used.
ab19043 has been referenced in 1 publication.
- Leuenberger N et al. Sumoylated PPARalpha mediates sex-specific gene repression and protects the liver from estrogen-induced toxicity in mice. J Clin Invest : (2009). WB ; Mouse . PubMed: 19729835