Anti-CYP8B1 antibody (ab191910)
Key features and details
- Rabbit polyclonal to CYP8B1
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-CYP8B1 antibody
See all CYP8B1 primary antibodies -
Description
Rabbit polyclonal to CYP8B1 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human CYP8B1.
Database link: Q9UNU6 -
Positive control
- HEK293T, RAW 264.7 and H9C2 whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab191910 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab191910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 58 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 58 kDa. |
Target
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Function
Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids. -
Tissue specificity
Liver. -
Sequence similarities
Belongs to the cytochrome P450 family. -
Cellular localization
Endoplasmic reticulum membrane. Microsome membrane. - Information by UniProt
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Database links
- Entrez Gene: 1582 Human
- Entrez Gene: 13124 Mouse
- Entrez Gene: 81924 Rat
- Omim: 602172 Human
- SwissProt: Q9UNU6 Human
- SwissProt: O88962 Mouse
- Unigene: 447793 Human
- Unigene: 722743 Human
see all -
Alternative names
- 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase antibody
- 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase antibody
- CP8B antibody
see all
Images
Datasheets and documents
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Datasheet download
References (16)
ab191910 has been referenced in 16 publications.
- Hu H et al. Gut microbiota promotes cholesterol gallstone formation by modulating bile acid composition and biliary cholesterol secretion. Nat Commun 13:252 (2022). PubMed: 35017486
- Zhuang Q et al. Gypenosides Prevent and Dissolve Cholesterol Gallstones by Modulating the Homeostasis of Cholesterol and Bile Acids. Front Med (Lausanne) 9:818144 (2022). PubMed: 35445045
- Qin W et al. Hepatic RACK1 deletion disturbs lipid and glucose homeostasis independently of insulin resistance. J Endocrinol 254:137-151 (2022). PubMed: 35608066
- Xiang H et al. Dynamics of the gut-liver axis in rats with varying fibrosis severity. Int J Biol Sci 18:3390-3404 (2022). PubMed: 35637968
- Jiang T et al. Linderae Radix Ethanol Extract Alleviates Diet-Induced Hyperlipidemia by Regulating Bile Acid Metabolism Through gut Microbiota. Front Pharmacol 12:627920 (2021). PubMed: 33679408