Overview

  • Product name
    Anti-CYP8B1 antibody - C-terminal
    See all CYP8B1 primary antibodies
  • Description
    Rabbit polyclonal to CYP8B1 - C-terminal
  • Host species
    Rabbit
  • Tested applications
    Suitable for: Flow Cyt, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human CYP8B1 aa 472-501 (C terminal) conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
    Database link: Q9UNU6

  • Positive control
    • K562 cell line lysate; Human hepatocarcinoma tissue; K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab175843 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10 - 1/50.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

 

IHC-P 1/10 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB 1/1000. Predicted molecular weight: 58 kDa.

Target

  • Function
    Involved in bile acid synthesis and is responsible for the conversion of 7 alpha-hydroxy-4-cholesten-3-one into 7 alpha, 12 alpha-dihydroxy-4-cholesten-3-one. Responsible for the balance between formation of cholic acid and chenodeoxycholic acid. Has a rather broad substrate specificity including a number of 7-alpha-hydroxylated C27 steroids.
  • Tissue specificity
    Liver.
  • Sequence similarities
    Belongs to the cytochrome P450 family.
  • Cellular localization
    Endoplasmic reticulum membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 7-alpha-hydroxy-4-cholesten-3-one 12-alpha-hydroxylase antibody
    • 7-alpha-hydroxycholest-4-en-3-one 12-alpha-hydroxylase antibody
    • CP8B antibody
    • CP8B1_HUMAN antibody
    • CYP12 antibody
    • CYP8B1 antibody
    • CYPVIIIB1 antibody
    • Cytochrome P450 8B1 antibody
    • Cytochrome P450 subfamily VIIIB (sterol 12 alpha hydroxylase) polypeptide 1 antibody
    • Cytochrome P450, family 8, subfamily B, polypeptide 1 antibody
    • Sterol 12-alpha-hydroxylase antibody
    see all

Images

  • Anti-CYP8B1 antibody - C-terminal (ab175843) at 1/1000 dilution + K562 cell line lysate at 35 µg

    Predicted band size: 58 kDa

  • Immunohistochemical analysis of paraffin embedded Human hepatocarcinoma tissue labeling CYP8B1 with ab175843 at 1/10 dilution, followed by peroxidase conjugation of the secondary antibody and DAB staining.

  • Flow Cytometrical analysis of K562 cells labeling CYP8B1 with ab175843 at 1/10 (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

References

ab175843 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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